CYC_NEUCR
ID CYC_NEUCR Reviewed; 108 AA.
AC P00048; Q7RV53;
DT 21-JUL-1986, integrated into UniProtKB/Swiss-Prot.
DT 23-JAN-2007, sequence version 2.
DT 03-AUG-2022, entry version 163.
DE RecName: Full=Cytochrome c;
GN Name=cyc-1; Synonyms=cyt-12; ORFNames=B1K11.020, NCU01808;
OS Neurospora crassa (strain ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 /
OS FGSC 987).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Sordariomycetes;
OC Sordariomycetidae; Sordariales; Sordariaceae; Neurospora.
OX NCBI_TaxID=367110;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA], AND VARIANT ASP-11.
RC STRAIN=74A;
RX PubMed=2820723; DOI=10.1002/j.1460-2075.1987.tb02480.x;
RA Stuart R.A., Neupert W., Tropschug M.;
RT "Deficiency in mRNA splicing in a cytochrome c mutant of Neurospora crassa:
RT importance of carboxy terminus for import of apocytochrome c into
RT mitochondria.";
RL EMBO J. 6:2131-2137(1987).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND VARIANT ASP-11.
RX PubMed=7882427; DOI=10.1007/bf00310497;
RA Bottorff D.A., Parmaksizoglu S., Lemire E.G., Coffin J.W., Bertrand H.,
RA Nargang F.E.;
RT "Mutations in the structural gene for cytochrome c result in deficiency of
RT both cytochromes aa3 and c in Neurospora crassa.";
RL Curr. Genet. 26:329-335(1994).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987;
RX PubMed=12655011; DOI=10.1093/nar/gkg293;
RA Mannhaupt G., Montrone C., Haase D., Mewes H.-W., Aign V., Hoheisel J.D.,
RA Fartmann B., Nyakatura G., Kempken F., Maier J., Schulte U.;
RT "What's in the genome of a filamentous fungus? Analysis of the Neurospora
RT genome sequence.";
RL Nucleic Acids Res. 31:1944-1954(2003).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987;
RX PubMed=12712197; DOI=10.1038/nature01554;
RA Galagan J.E., Calvo S.E., Borkovich K.A., Selker E.U., Read N.D.,
RA Jaffe D.B., FitzHugh W., Ma L.-J., Smirnov S., Purcell S., Rehman B.,
RA Elkins T., Engels R., Wang S., Nielsen C.B., Butler J., Endrizzi M.,
RA Qui D., Ianakiev P., Bell-Pedersen D., Nelson M.A., Werner-Washburne M.,
RA Selitrennikoff C.P., Kinsey J.A., Braun E.L., Zelter A., Schulte U.,
RA Kothe G.O., Jedd G., Mewes H.-W., Staben C., Marcotte E., Greenberg D.,
RA Roy A., Foley K., Naylor J., Stange-Thomann N., Barrett R., Gnerre S.,
RA Kamal M., Kamvysselis M., Mauceli E.W., Bielke C., Rudd S., Frishman D.,
RA Krystofova S., Rasmussen C., Metzenberg R.L., Perkins D.D., Kroken S.,
RA Cogoni C., Macino G., Catcheside D.E.A., Li W., Pratt R.J., Osmani S.A.,
RA DeSouza C.P.C., Glass N.L., Orbach M.J., Berglund J.A., Voelker R.,
RA Yarden O., Plamann M., Seiler S., Dunlap J.C., Radford A., Aramayo R.,
RA Natvig D.O., Alex L.A., Mannhaupt G., Ebbole D.J., Freitag M., Paulsen I.,
RA Sachs M.S., Lander E.S., Nusbaum C., Birren B.W.;
RT "The genome sequence of the filamentous fungus Neurospora crassa.";
RL Nature 422:859-868(2003).
RN [5]
RP PROTEIN SEQUENCE OF 2-108.
RX PubMed=5950080; DOI=10.1016/s0021-9258(18)96512-9;
RA Heller J., Smith E.L.;
RT "Neurospora crassa cytochrome c. II. Chymotryptic peptides, tryptic
RT peptides, cyanogen bromide peptides, and the complete amino acid
RT sequence.";
RL J. Biol. Chem. 241:3165-3180(1966).
RN [6]
RP PROTEIN SEQUENCE OF 2-44.
RX PubMed=4363050; DOI=10.1016/0006-291x(74)90844-4;
RA Lederer F., Simon A.M.;
RT "Neurospora crassa and Humicola lanuginosa cytochromes c: more homology in
RT the heme region.";
RL Biochem. Biophys. Res. Commun. 56:317-323(1974).
RN [7]
RP METHYLATION AT LYS-77.
RX PubMed=4304194; DOI=10.1016/s0021-9258(18)91855-7;
RA Delange R.J., Glazer A.N., Smith E.L.;
RT "Presence and location of an unusual amino acid, epsilon-N-trimethyllysine,
RT in cytochrome c of wheat germ and Neurospora.";
RL J. Biol. Chem. 244:1385-1388(1969).
CC -!- FUNCTION: Electron carrier protein. The oxidized form of the cytochrome
CC c heme group can accept an electron from the heme group of the
CC cytochrome c1 subunit of cytochrome reductase. Cytochrome c then
CC transfers this electron to the cytochrome oxidase complex, the final
CC protein carrier in the mitochondrial electron-transport chain.
CC -!- SUBCELLULAR LOCATION: Mitochondrion intermembrane space. Note=Loosely
CC associated with the inner membrane.
CC -!- PTM: Binds 1 heme c group covalently per subunit.
CC -!- SIMILARITY: Belongs to the cytochrome c family. {ECO:0000305}.
CC -!- WEB RESOURCE: Name=Protein Spotlight; Note=Life shuttle - Issue 76 of
CC November 2006;
CC URL="https://web.expasy.org/spotlight/back_issues/076";
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DR EMBL; X05506; CAA29050.1; -; mRNA.
DR EMBL; L19358; AAA92156.1; -; Genomic_DNA.
DR EMBL; X05507; CAA29051.1; -; Genomic_DNA.
DR EMBL; AL669998; CAD21169.1; -; Genomic_DNA.
DR EMBL; CM002237; EAA27250.2; -; Genomic_DNA.
DR PIR; S48221; CCNC.
DR RefSeq; XP_956486.2; XM_951393.3.
DR AlphaFoldDB; P00048; -.
DR SMR; P00048; -.
DR STRING; 5141.EFNCRP00000001740; -.
DR PRIDE; P00048; -.
DR EnsemblFungi; EAA27250; EAA27250; NCU01808.
DR GeneID; 3872635; -.
DR KEGG; ncr:NCU01808; -.
DR VEuPathDB; FungiDB:NCU01808; -.
DR HOGENOM; CLU_060944_3_0_1; -.
DR InParanoid; P00048; -.
DR Proteomes; UP000001805; Chromosome 6, Linkage Group II.
DR GO; GO:0005758; C:mitochondrial intermembrane space; IBA:GO_Central.
DR GO; GO:0070469; C:respirasome; IEA:UniProtKB-KW.
DR GO; GO:0009055; F:electron transfer activity; IEA:InterPro.
DR GO; GO:0020037; F:heme binding; IEA:InterPro.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0006123; P:mitochondrial electron transport, cytochrome c to oxygen; IBA:GO_Central.
DR GO; GO:0006122; P:mitochondrial electron transport, ubiquinol to cytochrome c; IBA:GO_Central.
DR Gene3D; 1.10.760.10; -; 1.
DR InterPro; IPR009056; Cyt_c-like_dom.
DR InterPro; IPR036909; Cyt_c-like_dom_sf.
DR InterPro; IPR002327; Cyt_c_1A/1B.
DR PANTHER; PTHR11961; PTHR11961; 1.
DR Pfam; PF00034; Cytochrom_C; 1.
DR PRINTS; PR00604; CYTCHRMECIAB.
DR SUPFAM; SSF46626; SSF46626; 1.
DR PROSITE; PS51007; CYTC; 1.
PE 1: Evidence at protein level;
KW Direct protein sequencing; Electron transport; Heme; Iron; Metal-binding;
KW Methylation; Mitochondrion; Reference proteome; Respiratory chain;
KW Transport.
FT INIT_MET 1
FT /note="Removed"
FT /evidence="ECO:0000269|PubMed:4363050,
FT ECO:0000269|PubMed:5950080"
FT CHAIN 2..108
FT /note="Cytochrome c"
FT /id="PRO_0000108328"
FT BINDING 19
FT /ligand="heme c"
FT /ligand_id="ChEBI:CHEBI:61717"
FT /note="covalent"
FT BINDING 22
FT /ligand="heme c"
FT /ligand_id="ChEBI:CHEBI:61717"
FT /note="covalent"
FT BINDING 23
FT /ligand="heme c"
FT /ligand_id="ChEBI:CHEBI:61717"
FT /ligand_part="Fe"
FT /ligand_part_id="ChEBI:CHEBI:18248"
FT /note="axial binding residue"
FT BINDING 85
FT /ligand="heme c"
FT /ligand_id="ChEBI:CHEBI:61717"
FT /ligand_part="Fe"
FT /ligand_part_id="ChEBI:CHEBI:18248"
FT /note="axial binding residue"
FT MOD_RES 77
FT /note="N6,N6,N6-trimethyllysine"
FT /evidence="ECO:0000269|PubMed:4304194"
FT VARIANT 11
FT /note="G -> D (in cyt-12-12/cyc-1-1; characterized by slow
FT growth and a deficiency of spectrophotometrically-
FT detectable cytochromes aa3 and c)"
FT /evidence="ECO:0000269|PubMed:2820723,
FT ECO:0000269|PubMed:7882427"
FT CONFLICT 21
FT /note="Q -> E (in Ref. 5; AA sequence)"
FT /evidence="ECO:0000305"
FT CONFLICT 24..31
FT /note="TLEEGGGN -> GEGGNLTQ (in Ref. 5; AA sequence)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 108 AA; 11813 MW; 8150D1A1B92A6E85 CRC64;
MGFSAGDSKK GANLFKTRCA QCHTLEEGGG NKIGPALHGL FGRKTGSVDG YAYTDANKQK
GITWDENTLF EYLENPKKYI PGTKMAFGGL KKDKDRNDII TFMKEATA
