CYF_LACSA
ID CYF_LACSA Reviewed; 320 AA.
AC Q332W5;
DT 06-FEB-2007, integrated into UniProtKB/Swiss-Prot.
DT 06-DEC-2005, sequence version 1.
DT 03-AUG-2022, entry version 75.
DE RecName: Full=Cytochrome f {ECO:0000255|HAMAP-Rule:MF_00610};
DE Flags: Precursor;
GN Name=petA {ECO:0000255|HAMAP-Rule:MF_00610};
OS Lactuca sativa (Garden lettuce).
OG Plastid; Chloroplast.
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC asterids; campanulids; Asterales; Asteraceae; Cichorioideae; Cichorieae;
OC Lactucinae; Lactuca.
OX NCBI_TaxID=4236;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Cisco;
RX PubMed=16604461; DOI=10.1007/s11248-005-3997-2;
RA Kanamoto H., Yamashita A., Asao H., Okumura S., Takase H., Hattori M.,
RA Yokota A., Tomizawa K.;
RT "Efficient and stable transformation of Lactuca sativa L. cv. Cisco
RT (lettuce) plastids.";
RL Transgenic Res. 15:205-217(2006).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Salinas;
RA Timme R.E., Kuehl J.V., Boore J.L., Jansen R.K.;
RT "A comparison of the first two published chloroplast genomes in Asteraceae:
RT Lactuca and Helianthus.";
RL Submitted (JAN-2006) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Component of the cytochrome b6-f complex, which mediates
CC electron transfer between photosystem II (PSII) and photosystem I
CC (PSI), cyclic electron flow around PSI, and state transitions.
CC {ECO:0000255|HAMAP-Rule:MF_00610}.
CC -!- COFACTOR:
CC Name=heme; Xref=ChEBI:CHEBI:30413;
CC Evidence={ECO:0000255|HAMAP-Rule:MF_00610};
CC Note=Binds 1 heme group covalently. {ECO:0000255|HAMAP-Rule:MF_00610};
CC -!- SUBUNIT: The 4 large subunits of the cytochrome b6-f complex are
CC cytochrome b6, subunit IV (17 kDa polypeptide, petD), cytochrome f and
CC the Rieske protein, while the 4 small subunits are PetG, PetL, PetM and
CC PetN. The complex functions as a dimer (By similarity). {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Plastid, chloroplast thylakoid membrane
CC {ECO:0000255|HAMAP-Rule:MF_00610}; Single-pass membrane protein
CC {ECO:0000255|HAMAP-Rule:MF_00610}.
CC -!- SIMILARITY: Belongs to the cytochrome f family. {ECO:0000255|HAMAP-
CC Rule:MF_00610}.
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DR EMBL; AP007232; BAE47607.1; -; Genomic_DNA.
DR EMBL; DQ383816; ABD47246.1; -; Genomic_DNA.
DR RefSeq; YP_398342.1; NC_007578.1.
DR AlphaFoldDB; Q332W5; -.
DR SMR; Q332W5; -.
DR PRIDE; Q332W5; -.
DR EnsemblPlants; rna-gnl|WGS:NBSK|LSAT_1X38720_mrna; cds-PLY97278.1; gene-LSAT_1X38720.
DR GeneID; 3772900; -.
DR Gramene; rna-gnl|WGS:NBSK|LSAT_1X38720_mrna; cds-PLY97278.1; gene-LSAT_1X38720.
DR KEGG; lsv:3772900; -.
DR OrthoDB; 826802at2759; -.
DR GO; GO:0009535; C:chloroplast thylakoid membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0031361; C:integral component of thylakoid membrane; IEA:InterPro.
DR GO; GO:0009055; F:electron transfer activity; IEA:UniProtKB-UniRule.
DR GO; GO:0020037; F:heme binding; IEA:InterPro.
DR GO; GO:0005506; F:iron ion binding; IEA:InterPro.
DR GO; GO:0015979; P:photosynthesis; IEA:UniProtKB-UniRule.
DR Gene3D; 2.60.40.830; -; 1.
DR HAMAP; MF_00610; Cytb6_f_cytF; 1.
DR InterPro; IPR024058; Cyt-f_TM.
DR InterPro; IPR002325; Cyt_f.
DR InterPro; IPR024094; Cyt_f_lg_dom.
DR InterPro; IPR036826; Cyt_f_lg_dom_sf.
DR InterPro; IPR011054; Rudment_hybrid_motif.
DR Pfam; PF01333; Apocytochr_F_C; 1.
DR Pfam; PF16639; Apocytochr_F_N; 1.
DR PRINTS; PR00610; CYTOCHROMEF.
DR SUPFAM; SSF103431; SSF103431; 1.
DR SUPFAM; SSF49441; SSF49441; 1.
DR SUPFAM; SSF51246; SSF51246; 1.
DR PROSITE; PS51010; CYTF; 1.
PE 3: Inferred from homology;
KW Chloroplast; Electron transport; Heme; Iron; Membrane; Metal-binding;
KW Photosynthesis; Plastid; Signal; Thylakoid; Transmembrane;
KW Transmembrane helix; Transport.
FT SIGNAL 1..35
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00610"
FT CHAIN 36..320
FT /note="Cytochrome f"
FT /id="PRO_0000275423"
FT TRANSMEM 286..306
FT /note="Helical"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00610"
FT BINDING 36
FT /ligand="heme"
FT /ligand_id="ChEBI:CHEBI:30413"
FT /ligand_part="Fe"
FT /ligand_part_id="ChEBI:CHEBI:18248"
FT /note="axial binding residue"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00610"
FT BINDING 56
FT /ligand="heme"
FT /ligand_id="ChEBI:CHEBI:30413"
FT /note="covalent"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00610"
FT BINDING 59
FT /ligand="heme"
FT /ligand_id="ChEBI:CHEBI:30413"
FT /note="covalent"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00610"
FT BINDING 60
FT /ligand="heme"
FT /ligand_id="ChEBI:CHEBI:30413"
FT /ligand_part="Fe"
FT /ligand_part_id="ChEBI:CHEBI:18248"
FT /note="axial binding residue"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00610"
SQ SEQUENCE 320 AA; 35319 MW; B1F79DB6C0D33522 CRC64;
MQTRNNFSWI KEQITRSISV SLMIYIITRA SISNAYPIFA QKGYENPREA TGRIVCANCH
LANKPVDIEV PQTVLPDTVF EAVVRIPYDM QLKQVLANGK KGALNVGAVL ILPEGFELAP
PDRISPEIKE KMGNLSFQSY RPNQKNILVI GPVPGQKYSE ITFPILSPDP ATKKDIHFLK
YPIYVGGNRG RGQIYPDGSK SNNTVYNATA SGIVSKILRK EKGGYEITIA DASDGRQVVD
IIPPGPELLV SEGESIKFEQ PLTSNPNVGG FGQGDAEIVL QDPLRVQGLL FFLASVILAQ
IFLVLKKKQF EKVQLSEMNF
