ACSA_DICDI
ID ACSA_DICDI Reviewed; 674 AA.
AC Q54Z60; Q86AW6;
DT 08-APR-2008, integrated into UniProtKB/Swiss-Prot.
DT 24-MAY-2005, sequence version 1.
DT 03-AUG-2022, entry version 93.
DE RecName: Full=Acetyl-coenzyme A synthetase;
DE EC=6.2.1.1;
DE AltName: Full=Acetate--CoA ligase;
DE AltName: Full=Acyl-activating enzyme;
GN Name=acsA; ORFNames=DDB_G0277815;
OS Dictyostelium discoideum (Slime mold).
OC Eukaryota; Amoebozoa; Evosea; Eumycetozoa; Dictyostelia; Dictyosteliales;
OC Dictyosteliaceae; Dictyostelium.
OX NCBI_TaxID=44689;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=AX4;
RX PubMed=12097910; DOI=10.1038/nature00847;
RA Gloeckner G., Eichinger L., Szafranski K., Pachebat J.A., Bankier A.T.,
RA Dear P.H., Lehmann R., Baumgart C., Parra G., Abril J.F., Guigo R.,
RA Kumpf K., Tunggal B., Cox E.C., Quail M.A., Platzer M., Rosenthal A.,
RA Noegel A.A.;
RT "Sequence and analysis of chromosome 2 of Dictyostelium discoideum.";
RL Nature 418:79-85(2002).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=AX4;
RX PubMed=15875012; DOI=10.1038/nature03481;
RA Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R.,
RA Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B.,
RA Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T.,
RA Lehmann R., Hamlin N., Davies R., Gaudet P., Fey P., Pilcher K., Chen G.,
RA Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N.,
RA Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E.,
RA Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N.,
RA Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D.,
RA Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T.,
RA Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D.,
RA Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A.,
RA Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M.,
RA Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A.,
RA Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y.,
RA Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C.,
RA Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R.,
RA Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.;
RT "The genome of the social amoeba Dictyostelium discoideum.";
RL Nature 435:43-57(2005).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=acetate + ATP + CoA = acetyl-CoA + AMP + diphosphate;
CC Xref=Rhea:RHEA:23176, ChEBI:CHEBI:30089, ChEBI:CHEBI:30616,
CC ChEBI:CHEBI:33019, ChEBI:CHEBI:57287, ChEBI:CHEBI:57288,
CC ChEBI:CHEBI:456215; EC=6.2.1.1;
CC -!- SIMILARITY: Belongs to the ATP-dependent AMP-binding enzyme family.
CC {ECO:0000305}.
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DR EMBL; AAFI02000022; EAL68581.1; -; Genomic_DNA.
DR RefSeq; XP_642493.1; XM_637401.1.
DR AlphaFoldDB; Q54Z60; -.
DR SMR; Q54Z60; -.
DR BioGRID; 1246207; 1.
DR STRING; 44689.DDB0231684; -.
DR PaxDb; Q54Z60; -.
DR EnsemblProtists; EAL68581; EAL68581; DDB_G0277815.
DR GeneID; 8621204; -.
DR KEGG; ddi:DDB_G0277815; -.
DR dictyBase; DDB_G0277815; acsA.
DR eggNOG; KOG1175; Eukaryota.
DR HOGENOM; CLU_000022_3_6_1; -.
DR InParanoid; Q54Z60; -.
DR OMA; DHWWHDL; -.
DR PhylomeDB; Q54Z60; -.
DR Reactome; R-DDI-71384; Ethanol oxidation.
DR PRO; PR:Q54Z60; -.
DR Proteomes; UP000002195; Chromosome 2.
DR GO; GO:0003987; F:acetate-CoA ligase activity; ISS:dictyBase.
DR GO; GO:0016208; F:AMP binding; ISS:dictyBase.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0006085; P:acetyl-CoA biosynthetic process; ISS:dictyBase.
DR GO; GO:0019427; P:acetyl-CoA biosynthetic process from acetate; IEA:InterPro.
DR Gene3D; 3.30.300.30; -; 1.
DR Gene3D; 3.40.50.12780; -; 1.
DR InterPro; IPR011904; Ac_CoA_lig.
DR InterPro; IPR032387; ACAS_N.
DR InterPro; IPR025110; AMP-bd_C.
DR InterPro; IPR045851; AMP-bd_C_sf.
DR InterPro; IPR020845; AMP-binding_CS.
DR InterPro; IPR000873; AMP-dep_Synth/Lig.
DR InterPro; IPR042099; ANL_N_sf.
DR Pfam; PF16177; ACAS_N; 1.
DR Pfam; PF00501; AMP-binding; 1.
DR Pfam; PF13193; AMP-binding_C; 1.
DR TIGRFAMs; TIGR02188; Ac_CoA_lig_AcsA; 1.
DR PROSITE; PS00455; AMP_BINDING; 1.
PE 3: Inferred from homology;
KW ATP-binding; Ligase; Nucleotide-binding; Reference proteome.
FT CHAIN 1..674
FT /note="Acetyl-coenzyme A synthetase"
FT /id="PRO_0000328354"
FT BINDING 201..204
FT /ligand="CoA"
FT /ligand_id="ChEBI:CHEBI:57287"
FT /evidence="ECO:0000250"
FT BINDING 320
FT /ligand="CoA"
FT /ligand_id="ChEBI:CHEBI:57287"
FT /evidence="ECO:0000250"
FT BINDING 396..398
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000250"
FT BINDING 420..425
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000250"
FT BINDING 518
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000250"
FT BINDING 533
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000250"
FT BINDING 541
FT /ligand="CoA"
FT /ligand_id="ChEBI:CHEBI:57287"
FT /evidence="ECO:0000250"
FT BINDING 544
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000250"
FT BINDING 603
FT /ligand="CoA"
FT /ligand_id="ChEBI:CHEBI:57287"
FT /evidence="ECO:0000250"
SQ SEQUENCE 674 AA; 75586 MW; 4DF825F016ECAB8F CRC64;
MQITEKETVQ EEQQKLLYPP PSFSTKCHIS SVEQYNEMYK ESIESPNQFW DKKAKEFLTW
FSDYTTVQHG SFEKGDISWF LNGKINVSYN CIDRHLKENA DKVAILFEGD EETMVKKVTY
REMFEEVCRL SNLLISLGVG KGDTVAIYLP NTPTAIYSML ACARIGAIHS VIFAGFGYES
IVSRVHDAKC RVIITADEGL RGGRYIPLKE KIDQVVQHCK LVQHVLVFKN TGRPTITFNP
SIDIWADEAM LDHRPYCPPV WLDSEDPLFI LYTSGSTGTP KGLVHTQAGY LLYAAMTHRY
VFDYHDSDVY ACMADVGWIT GHSYIVYGPL ANGATTFIFE GTPLYPTPAR YWEMVQRHKI
TQFYTAPTAI RSLMKFPISF TQQSDKSSLR VLGSVGEPIN PEAWRWFNTN VGEGRCAIVD
TYWQTESGGH LITPLPGVTS TKPGSATKPF FGIELQVLDS KTGERLYINP DINGCKEISG
VLAISKPWPG IARSVYRSHG RYLQTYMTQY KGHYFTGDGV KLDSDGYYWI EGRVDDVINV
SGHRLGTAEL ESALVGCSIC AEAAVVGYPH DIKGQGILAF CTLKEGYQED ESNVIMMLKK
EVRNVIGPFA TPDVIVITPS LPKTRSGKIM RRILRKIGCH ESSAEQLGDI STLAEPEVVK
LLIEKVSKVI PKTH
