ID   CYHR_CANMA              Reviewed;         552 AA.
AC   P32071;
DT   01-OCT-1993, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-1993, sequence version 1.
DT   25-MAY-2022, entry version 82.
DE   RecName: Full=Cycloheximide resistance protein;
GN   Name=CYHR;
OS   Candida maltosa (Yeast).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC   Saccharomycetales; Debaryomycetaceae; Candida/Lodderomyces clade; Candida.
OX   NCBI_TaxID=5479;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=VSB-889;
RX   PubMed=1628836; DOI=10.1016/0378-1119(92)90636-4;
RA   Sasnauskas K., Jomantiene R., Lebediene E., Lebedys J., Januska A.,
RA   Janulaitis A.;
RT   "Cloning and sequence analysis of a Candida maltosa gene which confers
RT   resistance to cycloheximide.";
RL   Gene 116:105-108(1992).
CC   -!- FUNCTION: Probable transporter. Confers resistance to cycloheximide.
CC   -!- SUBCELLULAR LOCATION: Membrane; Multi-pass membrane protein.
CC   -!- SIMILARITY: Belongs to the major facilitator superfamily. CAR1 family.
CC       {ECO:0000305}.
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DR   EMBL; M64932; AAA34335.1; -; Genomic_DNA.
DR   PIR; JC1173; JC1173.
DR   AlphaFoldDB; P32071; -.
DR   TCDB; 2.A.1.2.2; the major facilitator superfamily (mfs).
DR   PRIDE; P32071; -.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0042910; F:xenobiotic transmembrane transporter activity; IEA:InterPro.
DR   GO; GO:0046677; P:response to antibiotic; IEA:UniProtKB-KW.
DR   GO; GO:0046898; P:response to cycloheximide; IEA:UniProtKB-KW.
DR   Gene3D; 1.20.1250.20; -; 1.
DR   InterPro; IPR011701; MFS.
DR   InterPro; IPR020846; MFS_dom.
DR   InterPro; IPR036259; MFS_trans_sf.
DR   InterPro; IPR004734; Multidrug-R.
DR   Pfam; PF07690; MFS_1; 1.
DR   SUPFAM; SSF103473; SSF103473; 1.
DR   TIGRFAMs; TIGR00880; 2_A_01_02; 1.
DR   PROSITE; PS50850; MFS; 1.
PE   3: Inferred from homology;
KW   Antibiotic resistance; Cycloheximide resistance; Membrane; Transmembrane;
KW   Transmembrane helix; Transport.
FT   CHAIN           1..552
FT                   /note="Cycloheximide resistance protein"
FT                   /id="PRO_0000173434"
FT   TRANSMEM        100..120
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        137..157
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        168..188
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        194..213
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        225..246
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        262..282
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        346..362
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        381..399
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        419..439
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        445..464
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        477..494
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        518..539
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   REGION          46..70
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        51..70
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   552 AA;  61366 MW;  4D1BF43B4010EF03 CRC64;
     MAAFIKDSFW GQIIYRLSGR KLFRHNDELP DYVVPEKYLL DPKEEVLNSS DKSQSSENKE
     QTEGDQATIQ NEPASEHIIV TWDGDDDPEN PYNWPFAWKA IAAMQIGFLT VSVYMASAIY
     TPGVEEIMNQ FNINSTLATL PLTMFVIGYG IGPLFWSPLS ENSRIGRTPL YIITLFIFFI
     LQIPTALSNH IAGLSVLRVI AGFFAAPALS TGGASYGDFI AMHYYSIALG VWSIFAVAGP
     SIGPLIGAAV INRSHDADGW RWSFWFMAIL SGVCFIVLSF SLPETYGKTL LRRKAERLRK
     LTGNNRIISE GELEDGHKTT SQVVSSLLWR PLEITMLEPV VFLIDIYIAL VYSIMYLIFE
     SVPIVYAGIH HFTLVEMGAT YVSTIIGIII GGAIYLPTVY YKFTKKLLAG QNVTPEVFLP
     PAIFGAICMP IGVFIFGWTS SPDINWFVPL IGMALFAVGA FIIFQTLFNY MAVSFKVEYL
     ASVFSSNAFF RSVSAGAFPL FGRALYNNLS IDKFPVGWGS SILGFISLGM IAIPVFFYLN
     GPKLRARSKY AY