ID   CYLA_PSYLO              Reviewed;          31 AA.
AC   P56872; P82254;
DT   30-MAY-2000, integrated into UniProtKB/Swiss-Prot.
DT   05-DEC-2001, sequence version 2.
DT   25-MAY-2022, entry version 59.
DE   RecName: Full=Cyclopsychotride-A;
DE            Short=CPT;
OS   Psychotria longipes.
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   asterids; lamiids; Gentianales; Rubiaceae; Rubioideae; Psychotrieae;
OC   Psychotria.
OX   NCBI_TaxID=41680;
RN   [1]
RP   PROTEIN SEQUENCE.
RX   PubMed=7714530; DOI=10.1021/np50114a002;
RA   Witherup K.M., Bogusky M.J., Anderson P.S., Ramjit H., Ransom R.W.,
RA   Wood T., Sardana M.;
RT   "Cyclopsychotride A, a biologically active, 31-residue cyclic peptide
RT   isolated from Psychotria longipes.";
RL   J. Nat. Prod. 57:1619-1625(1994).
RN   [2]
RP   SYNTHESIS, AND ANTIBACTERIAL ACTIVITY.
RX   PubMed=10430870; DOI=10.1073/pnas.96.16.8913;
RA   Tam J.P., Lu Y.-A., Yang J.-L., Chiu K.-W.;
RT   "An unusual structural motif of antimicrobial peptides containing end-to-
RT   end macrocycle and cystine-knot disulfides.";
RL   Proc. Natl. Acad. Sci. U.S.A. 96:8913-8918(1999).
CC   -!- FUNCTION: Probably participates in a plant defense mechanism. Has
CC       antibiotic activity. Inhibits the cytopathic effects and replication of
CC       the human immunodeficiency virus. Active against both Gram-positive and
CC       Gram-negative bacteria.
CC   -!- DOMAIN: The presence of a 'disulfide through disulfide knot'
CC       structurally defines this protein as a knottin. {ECO:0000250}.
CC   -!- PTM: This is a cyclic peptide.
CC   -!- SIMILARITY: Belongs to the cyclotide family. Bracelet subfamily.
CC       {ECO:0000255|PROSITE-ProRule:PRU00395}.
CC   -!- CAUTION: This peptide is cyclic. The start position was chosen by
CC       similarity to OAK1 (kalata-B1) for which the DNA sequence is known.
CC       {ECO:0000305}.
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DR   AlphaFoldDB; P56872; -.
DR   SMR; P56872; -.
DR   GO; GO:0042742; P:defense response to bacterium; IEA:UniProtKB-KW.
DR   InterPro; IPR005535; Cyclotide.
DR   InterPro; IPR012323; Cyclotide_bracelet_CS.
DR   InterPro; IPR036146; Cyclotide_sf.
DR   Pfam; PF03784; Cyclotide; 1.
DR   PIRSF; PIRSF037891; Cycloviolacin; 1.
DR   SUPFAM; SSF57038; SSF57038; 1.
DR   PROSITE; PS51052; CYCLOTIDE; 1.
DR   PROSITE; PS60008; CYCLOTIDE_BRACELET; 1.
PE   1: Evidence at protein level;
KW   Antibiotic; Antimicrobial; Direct protein sequencing; Disulfide bond;
KW   Knottin; Plant defense.
FT   PEPTIDE         1..31
FT                   /note="Cyclopsychotride-A"
FT                   /id="PRO_0000044698"
FT   DISULFID        4..21
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00395"
FT   DISULFID        8..23
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00395"
FT   DISULFID        13..28
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00395"
FT   CROSSLNK        1..31
FT                   /note="Cyclopeptide (Ser-Asn)"
SQ   SEQUENCE   31 AA;  3255 MW;  E44180BAB37403FE CRC64;
     SIPCGESCVF IPCTVTALLG CSCKSKVCYK N