CYNS_AQUAE
ID CYNS_AQUAE Reviewed; 149 AA.
AC O66587;
DT 31-JAN-2002, integrated into UniProtKB/Swiss-Prot.
DT 01-AUG-1998, sequence version 1.
DT 03-AUG-2022, entry version 113.
DE RecName: Full=Cyanate hydratase {ECO:0000255|HAMAP-Rule:MF_00535};
DE Short=Cyanase {ECO:0000255|HAMAP-Rule:MF_00535};
DE EC=4.2.1.104 {ECO:0000255|HAMAP-Rule:MF_00535};
DE AltName: Full=Cyanate hydrolase {ECO:0000255|HAMAP-Rule:MF_00535};
DE AltName: Full=Cyanate lyase {ECO:0000255|HAMAP-Rule:MF_00535};
GN Name=cynS {ECO:0000255|HAMAP-Rule:MF_00535}; OrderedLocusNames=aq_212;
OS Aquifex aeolicus (strain VF5).
OC Bacteria; Aquificae; Aquificales; Aquificaceae; Aquifex.
OX NCBI_TaxID=224324;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=VF5;
RX PubMed=9537320; DOI=10.1038/32831;
RA Deckert G., Warren P.V., Gaasterland T., Young W.G., Lenox A.L.,
RA Graham D.E., Overbeek R., Snead M.A., Keller M., Aujay M., Huber R.,
RA Feldman R.A., Short J.M., Olsen G.J., Swanson R.V.;
RT "The complete genome of the hyperthermophilic bacterium Aquifex aeolicus.";
RL Nature 392:353-358(1998).
CC -!- FUNCTION: Catalyzes the reaction of cyanate with bicarbonate to produce
CC ammonia and carbon dioxide. {ECO:0000255|HAMAP-Rule:MF_00535}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=cyanate + 3 H(+) + hydrogencarbonate = 2 CO2 + NH4(+);
CC Xref=Rhea:RHEA:11120, ChEBI:CHEBI:15378, ChEBI:CHEBI:16526,
CC ChEBI:CHEBI:17544, ChEBI:CHEBI:28938, ChEBI:CHEBI:29195;
CC EC=4.2.1.104; Evidence={ECO:0000255|HAMAP-Rule:MF_00535};
CC -!- SIMILARITY: Belongs to the cyanase family. {ECO:0000255|HAMAP-
CC Rule:MF_00535}.
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DR EMBL; AE000657; AAC06548.1; -; Genomic_DNA.
DR PIR; G70319; G70319.
DR RefSeq; NP_213147.1; NC_000918.1.
DR RefSeq; WP_010880085.1; NC_000918.1.
DR AlphaFoldDB; O66587; -.
DR SMR; O66587; -.
DR STRING; 224324.aq_212; -.
DR PRIDE; O66587; -.
DR EnsemblBacteria; AAC06548; AAC06548; aq_212.
DR KEGG; aae:aq_212; -.
DR PATRIC; fig|224324.8.peg.178; -.
DR eggNOG; COG1513; Bacteria.
DR HOGENOM; CLU_103452_1_0_0; -.
DR InParanoid; O66587; -.
DR OMA; YELVMIN; -.
DR OrthoDB; 1506439at2; -.
DR Proteomes; UP000000798; Chromosome.
DR GO; GO:0008824; F:cyanate hydratase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0003677; F:DNA binding; IEA:InterPro.
DR GO; GO:0009439; P:cyanate metabolic process; IEA:UniProtKB-UniRule.
DR CDD; cd00559; Cyanase_C; 1.
DR Gene3D; 1.10.260.40; -; 1.
DR Gene3D; 3.30.1160.10; -; 1.
DR HAMAP; MF_00535; Cyanate_hydrat; 1.
DR InterPro; IPR001387; Cro/C1-type_HTH.
DR InterPro; IPR008076; Cyanase.
DR InterPro; IPR003712; Cyanate_lyase_C.
DR InterPro; IPR036581; Cyanate_lyase_C_sf.
DR InterPro; IPR010982; Lambda_DNA-bd_dom_sf.
DR PANTHER; PTHR34186; PTHR34186; 1.
DR Pfam; PF02560; Cyanate_lyase; 1.
DR Pfam; PF01381; HTH_3; 1.
DR PIRSF; PIRSF001263; Cyanate_hydratas; 1.
DR PRINTS; PR01693; CYANASE.
DR SMART; SM01116; Cyanate_lyase; 1.
DR SMART; SM00530; HTH_XRE; 1.
DR SUPFAM; SSF47413; SSF47413; 1.
DR SUPFAM; SSF55234; SSF55234; 1.
DR TIGRFAMs; TIGR00673; cynS; 1.
PE 3: Inferred from homology;
KW Lyase; Reference proteome.
FT CHAIN 1..149
FT /note="Cyanate hydratase"
FT /id="PRO_0000187521"
FT ACT_SITE 90
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00535"
FT ACT_SITE 93
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00535"
FT ACT_SITE 116
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00535"
SQ SEQUENCE 149 AA; 17201 MW; 0E6F5F4FCF340A5C CRC64;
MRSDIGRLSK YLIERKKNLG LTWEDVSRKL GKSPVYCAML FYGYAQADDE EVKAVAELLN
LEEKELAELK DAPYREPQQP VPPTDPFVYR LYEVVILYGP ALKDVAHEMF GDGIMSAIDM
SVELEKVEQE GAERMVLTFN GKWLKYRKF
