CYNS_ARATH
ID CYNS_ARATH Reviewed; 168 AA.
AC O22683;
DT 31-JAN-2002, integrated into UniProtKB/Swiss-Prot.
DT 01-JAN-1998, sequence version 1.
DT 03-AUG-2022, entry version 140.
DE RecName: Full=Cyanate hydratase {ECO:0000255|HAMAP-Rule:MF_03139};
DE Short=Cyanase {ECO:0000255|HAMAP-Rule:MF_03139};
DE EC=4.2.1.104 {ECO:0000255|HAMAP-Rule:MF_03139};
DE AltName: Full=Cyanate hydrolase {ECO:0000255|HAMAP-Rule:MF_03139};
DE AltName: Full=Cyanate lyase {ECO:0000255|HAMAP-Rule:MF_03139};
GN Name=CYN {ECO:0000255|HAMAP-Rule:MF_03139}; OrderedLocusNames=At3g23490;
GN ORFNames=MEE5.3;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RA Aichi M., Nishida I., Omata T.;
RT "Molecular cloning and characterization of a cDNA encoding cyanase from
RT Arabidopsis thaliana.";
RL Submitted (JUN-1997) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=cv. Columbia;
RA Aichi M., Nishida I., Omata T.;
RT "Arabidopsis thaliana gene for cyanase.";
RL Submitted (JUN-1998) to the EMBL/GenBank/DDBJ databases.
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10907853; DOI=10.1093/dnares/7.3.217;
RA Kaneko T., Katoh T., Sato S., Nakamura Y., Asamizu E., Tabata S.;
RT "Structural analysis of Arabidopsis thaliana chromosome 3. II. Sequence
RT features of the 4,251,695 bp regions covered by 90 P1, TAC and BAC
RT clones.";
RL DNA Res. 7:217-221(2000).
RN [4]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
CC -!- FUNCTION: Catalyzes the reaction of cyanate with bicarbonate to produce
CC ammonia and carbon dioxide. {ECO:0000255|HAMAP-Rule:MF_03139}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=cyanate + 3 H(+) + hydrogencarbonate = 2 CO2 + NH4(+);
CC Xref=Rhea:RHEA:11120, ChEBI:CHEBI:15378, ChEBI:CHEBI:16526,
CC ChEBI:CHEBI:17544, ChEBI:CHEBI:28938, ChEBI:CHEBI:29195;
CC EC=4.2.1.104; Evidence={ECO:0000255|HAMAP-Rule:MF_03139};
CC -!- SIMILARITY: Belongs to the cyanase family. {ECO:0000255|HAMAP-
CC Rule:MF_03139}.
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DR EMBL; AB004568; BAA21660.1; -; mRNA.
DR EMBL; AB015748; BAA31224.1; -; Genomic_DNA.
DR EMBL; AP000374; BAB01741.1; -; Genomic_DNA.
DR EMBL; CP002686; AEE76771.1; -; Genomic_DNA.
DR EMBL; CP002686; ANM65955.1; -; Genomic_DNA.
DR EMBL; AF410298; AAK95284.1; -; mRNA.
DR EMBL; AY093708; AAM10332.1; -; mRNA.
DR RefSeq; NP_001327887.1; NM_001338643.1.
DR RefSeq; NP_188991.1; NM_113252.4.
DR AlphaFoldDB; O22683; -.
DR SMR; O22683; -.
DR BioGRID; 7262; 1.
DR STRING; 3702.AT3G23490.1; -.
DR iPTMnet; O22683; -.
DR PaxDb; O22683; -.
DR PRIDE; O22683; -.
DR ProteomicsDB; 222670; -.
DR EnsemblPlants; AT3G23490.1; AT3G23490.1; AT3G23490.
DR EnsemblPlants; AT3G23490.2; AT3G23490.2; AT3G23490.
DR GeneID; 821930; -.
DR Gramene; AT3G23490.1; AT3G23490.1; AT3G23490.
DR Gramene; AT3G23490.2; AT3G23490.2; AT3G23490.
DR KEGG; ath:AT3G23490; -.
DR Araport; AT3G23490; -.
DR TAIR; locus:2088727; AT3G23490.
DR eggNOG; ENOG502S3I5; Eukaryota.
DR HOGENOM; CLU_103452_2_0_1; -.
DR InParanoid; O22683; -.
DR OMA; YELVMIN; -.
DR OrthoDB; 1486663at2759; -.
DR PhylomeDB; O22683; -.
DR BioCyc; ARA:AT3G23490-MON; -.
DR BioCyc; MetaCyc:AT3G23490-MON; -.
DR BRENDA; 4.2.1.104; 399.
DR PRO; PR:O22683; -.
DR Proteomes; UP000006548; Chromosome 3.
DR ExpressionAtlas; O22683; baseline and differential.
DR Genevisible; O22683; AT.
DR GO; GO:0005829; C:cytosol; HDA:TAIR.
DR GO; GO:0008824; F:cyanate hydratase activity; IDA:TAIR.
DR GO; GO:0003677; F:DNA binding; IEA:InterPro.
DR GO; GO:0042802; F:identical protein binding; IPI:TAIR.
DR GO; GO:0009440; P:cyanate catabolic process; IMP:TAIR.
DR GO; GO:0009651; P:response to salt stress; IEP:TAIR.
DR CDD; cd00559; Cyanase_C; 1.
DR Gene3D; 1.10.260.40; -; 1.
DR Gene3D; 3.30.1160.10; -; 1.
DR HAMAP; MF_00535; Cyanate_hydrat; 1.
DR InterPro; IPR008076; Cyanase.
DR InterPro; IPR003712; Cyanate_lyase_C.
DR InterPro; IPR036581; Cyanate_lyase_C_sf.
DR InterPro; IPR010982; Lambda_DNA-bd_dom_sf.
DR PANTHER; PTHR34186; PTHR34186; 1.
DR Pfam; PF02560; Cyanate_lyase; 1.
DR PIRSF; PIRSF001263; Cyanate_hydratas; 1.
DR PRINTS; PR01693; CYANASE.
DR SMART; SM01116; Cyanate_lyase; 1.
DR SUPFAM; SSF47413; SSF47413; 1.
DR SUPFAM; SSF55234; SSF55234; 1.
DR TIGRFAMs; TIGR00673; cynS; 1.
PE 2: Evidence at transcript level;
KW Lyase; Reference proteome.
FT CHAIN 1..168
FT /note="Cyanate hydratase"
FT /id="PRO_0000187534"
FT ACT_SITE 91
FT /evidence="ECO:0000255|HAMAP-Rule:MF_03139"
FT ACT_SITE 94
FT /evidence="ECO:0000255|HAMAP-Rule:MF_03139"
FT ACT_SITE 117
FT /evidence="ECO:0000255|HAMAP-Rule:MF_03139"
SQ SEQUENCE 168 AA; 18592 MW; B31E891C80A7789E CRC64;
MEAAKKQSVT NQLLAVKSAS GKTFSQLAAE TGLTNVYVAQ LLRRQAQLKP DTVPKLKEAL
PALTDELIGD MMSPPWRSYD PNLIQEPTIY RLNEAVMHFG ESIKEIINED FGDGIMSAID
FYCSVDKIKG VDGNNRVVVT LDGKYLSHSE QRTENMVSRL NLKGGTSE
