CYNS_ASPFN
ID CYNS_ASPFN Reviewed; 160 AA.
AC B8NQ08;
DT 11-JAN-2011, integrated into UniProtKB/Swiss-Prot.
DT 03-MAR-2009, sequence version 1.
DT 03-AUG-2022, entry version 66.
DE RecName: Full=Cyanate hydratase {ECO:0000255|HAMAP-Rule:MF_03139};
DE Short=Cyanase {ECO:0000255|HAMAP-Rule:MF_03139};
DE EC=4.2.1.104 {ECO:0000255|HAMAP-Rule:MF_03139};
DE AltName: Full=Cyanate hydrolase {ECO:0000255|HAMAP-Rule:MF_03139};
DE AltName: Full=Cyanate lyase {ECO:0000255|HAMAP-Rule:MF_03139};
GN Name=cyn1 {ECO:0000255|HAMAP-Rule:MF_03139}; ORFNames=AFLA_003110;
OS Aspergillus flavus (strain ATCC 200026 / FGSC A1120 / IAM 13836 / NRRL 3357
OS / JCM 12722 / SRRC 167).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Eurotiomycetes;
OC Eurotiomycetidae; Eurotiales; Aspergillaceae; Aspergillus;
OC Aspergillus subgen. Circumdati.
OX NCBI_TaxID=332952;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 200026 / FGSC A1120 / IAM 13836 / NRRL 3357 / JCM 12722 / SRRC
RC 167;
RX PubMed=25883274; DOI=10.1128/genomea.00168-15;
RA Nierman W.C., Yu J., Fedorova-Abrams N.D., Losada L., Cleveland T.E.,
RA Bhatnagar D., Bennett J.W., Dean R., Payne G.A.;
RT "Genome sequence of Aspergillus flavus NRRL 3357, a strain that causes
RT aflatoxin contamination of food and feed.";
RL Genome Announc. 3:E0016815-E0016815(2015).
CC -!- FUNCTION: Catalyzes the reaction of cyanate with bicarbonate to produce
CC ammonia and carbon dioxide. {ECO:0000255|HAMAP-Rule:MF_03139}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=cyanate + 3 H(+) + hydrogencarbonate = 2 CO2 + NH4(+);
CC Xref=Rhea:RHEA:11120, ChEBI:CHEBI:15378, ChEBI:CHEBI:16526,
CC ChEBI:CHEBI:17544, ChEBI:CHEBI:28938, ChEBI:CHEBI:29195;
CC EC=4.2.1.104; Evidence={ECO:0000255|HAMAP-Rule:MF_03139};
CC -!- SIMILARITY: Belongs to the cyanase family. {ECO:0000255|HAMAP-
CC Rule:MF_03139}.
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DR EMBL; EQ963482; EED47670.1; -; Genomic_DNA.
DR RefSeq; XP_002382512.1; XM_002382471.1.
DR AlphaFoldDB; B8NQ08; -.
DR SMR; B8NQ08; -.
DR STRING; 5059.CADAFLAP00010377; -.
DR EnsemblFungi; EED47670; EED47670; AFLA_003110.
DR VEuPathDB; FungiDB:AFLA_003110; -.
DR eggNOG; ENOG502S3YJ; Eukaryota.
DR HOGENOM; CLU_103452_0_0_1; -.
DR OMA; YELVMIN; -.
DR Proteomes; UP000001875; Unassembled WGS sequence.
DR GO; GO:0008824; F:cyanate hydratase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0003677; F:DNA binding; IEA:InterPro.
DR GO; GO:0009439; P:cyanate metabolic process; IEA:UniProtKB-UniRule.
DR CDD; cd00559; Cyanase_C; 1.
DR Gene3D; 1.10.260.40; -; 1.
DR Gene3D; 3.30.1160.10; -; 1.
DR HAMAP; MF_00535; Cyanate_hydrat; 1.
DR InterPro; IPR008076; Cyanase.
DR InterPro; IPR003712; Cyanate_lyase_C.
DR InterPro; IPR036581; Cyanate_lyase_C_sf.
DR InterPro; IPR010982; Lambda_DNA-bd_dom_sf.
DR PANTHER; PTHR34186; PTHR34186; 1.
DR Pfam; PF02560; Cyanate_lyase; 1.
DR PIRSF; PIRSF001263; Cyanate_hydratas; 1.
DR PRINTS; PR01693; CYANASE.
DR SMART; SM01116; Cyanate_lyase; 1.
DR SUPFAM; SSF47413; SSF47413; 1.
DR SUPFAM; SSF55234; SSF55234; 1.
DR TIGRFAMs; TIGR00673; cynS; 1.
PE 3: Inferred from homology;
KW Lyase.
FT CHAIN 1..160
FT /note="Cyanate hydratase"
FT /id="PRO_0000403240"
FT ACT_SITE 100
FT /evidence="ECO:0000255|HAMAP-Rule:MF_03139"
FT ACT_SITE 103
FT /evidence="ECO:0000255|HAMAP-Rule:MF_03139"
FT ACT_SITE 126
FT /evidence="ECO:0000255|HAMAP-Rule:MF_03139"
SQ SEQUENCE 160 AA; 17749 MW; A6ADA83484934E4A CRC64;
MSLATLDTSQ HPNLPSASAT LFKAKAAKKF SFEQIAQHIG RNEVATAAIF YGQAKASPED
ITNLASLLEI PQEVLEDQLS GFPDRGKSVE MPPKEPLIYR LYEIVQNYGY AYKAVLNEKF
GDGIMSAISF STKVEKETDA DGNNWAVITL RGKWLPFSRF
