CYNS_BURCM
ID CYNS_BURCM Reviewed; 156 AA.
AC Q0B2Y2;
DT 15-JAN-2008, integrated into UniProtKB/Swiss-Prot.
DT 17-OCT-2006, sequence version 1.
DT 25-MAY-2022, entry version 84.
DE RecName: Full=Cyanate hydratase {ECO:0000255|HAMAP-Rule:MF_00535};
DE Short=Cyanase {ECO:0000255|HAMAP-Rule:MF_00535};
DE EC=4.2.1.104 {ECO:0000255|HAMAP-Rule:MF_00535};
DE AltName: Full=Cyanate hydrolase {ECO:0000255|HAMAP-Rule:MF_00535};
DE AltName: Full=Cyanate lyase {ECO:0000255|HAMAP-Rule:MF_00535};
GN Name=cynS {ECO:0000255|HAMAP-Rule:MF_00535}; OrderedLocusNames=Bamb_5946;
OS Burkholderia ambifaria (strain ATCC BAA-244 / AMMD) (Burkholderia cepacia
OS (strain AMMD)).
OC Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
OC Burkholderiaceae; Burkholderia; Burkholderia cepacia complex.
OX NCBI_TaxID=339670;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC BAA-244 / AMMD;
RA Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C.,
RA Glavina del Rio T., Hammon N., Israni S., Pitluck S., Bruce D., Chain P.,
RA Malfatti S., Shin M., Vergez L., Schmutz J., Larimer F., Land M.,
RA Hauser L., Kyrpides N., Kim E., Parke J., Coenye T., Konstantinidis K.,
RA Ramette A., Tiedje J., Richardson P.;
RT "Complete sequence of chromosome 3 of Burkholderia cepacia AMMD.";
RL Submitted (AUG-2006) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Catalyzes the reaction of cyanate with bicarbonate to produce
CC ammonia and carbon dioxide. {ECO:0000255|HAMAP-Rule:MF_00535}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=cyanate + 3 H(+) + hydrogencarbonate = 2 CO2 + NH4(+);
CC Xref=Rhea:RHEA:11120, ChEBI:CHEBI:15378, ChEBI:CHEBI:16526,
CC ChEBI:CHEBI:17544, ChEBI:CHEBI:28938, ChEBI:CHEBI:29195;
CC EC=4.2.1.104; Evidence={ECO:0000255|HAMAP-Rule:MF_00535};
CC -!- SIMILARITY: Belongs to the cyanase family. {ECO:0000255|HAMAP-
CC Rule:MF_00535}.
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DR EMBL; CP000442; ABI91491.1; -; Genomic_DNA.
DR RefSeq; WP_011660834.1; NZ_CP009800.1.
DR AlphaFoldDB; Q0B2Y2; -.
DR SMR; Q0B2Y2; -.
DR STRING; 339670.Bamb_5946; -.
DR EnsemblBacteria; ABI91491; ABI91491; Bamb_5946.
DR GeneID; 44696486; -.
DR KEGG; bam:Bamb_5946; -.
DR PATRIC; fig|339670.21.peg.6905; -.
DR eggNOG; COG1513; Bacteria.
DR OMA; YELVMIN; -.
DR Proteomes; UP000000662; Chromosome 3.
DR GO; GO:0008824; F:cyanate hydratase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0003677; F:DNA binding; IEA:InterPro.
DR GO; GO:0009439; P:cyanate metabolic process; IEA:UniProtKB-UniRule.
DR CDD; cd00559; Cyanase_C; 1.
DR Gene3D; 1.10.260.40; -; 1.
DR Gene3D; 3.30.1160.10; -; 1.
DR HAMAP; MF_00535; Cyanate_hydrat; 1.
DR InterPro; IPR008076; Cyanase.
DR InterPro; IPR003712; Cyanate_lyase_C.
DR InterPro; IPR036581; Cyanate_lyase_C_sf.
DR InterPro; IPR010982; Lambda_DNA-bd_dom_sf.
DR PANTHER; PTHR34186; PTHR34186; 1.
DR Pfam; PF02560; Cyanate_lyase; 1.
DR PIRSF; PIRSF001263; Cyanate_hydratas; 1.
DR PRINTS; PR01693; CYANASE.
DR SMART; SM01116; Cyanate_lyase; 1.
DR SUPFAM; SSF47413; SSF47413; 1.
DR SUPFAM; SSF55234; SSF55234; 1.
DR TIGRFAMs; TIGR00673; cynS; 1.
PE 3: Inferred from homology;
KW Lyase.
FT CHAIN 1..156
FT /note="Cyanate hydratase"
FT /id="PRO_1000051466"
FT ACT_SITE 96
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00535"
FT ACT_SITE 99
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00535"
FT ACT_SITE 122
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00535"
SQ SEQUENCE 156 AA; 16893 MW; 0234D29172DA5656 CRC64;
MTQSQVTPNA REALTETIVD AKVRKNLTFE AINEGTGLSL AYTTAALLGQ HALPEKAAKL
VAERLGLDDD AVRLLQTIPV RGSIPGGVPT DPTVYRFYEM VQVYGSTLKA LVHEKFGDGI
ISAINFKLDI QKVPDPEGGE RAVITLNGKY LPTKPF
