ID   CYNS_MAIZE              Reviewed;         166 AA.
AC   B6TTW1; C4J2E0;
DT   11-JAN-2011, integrated into UniProtKB/Swiss-Prot.
DT   16-DEC-2008, sequence version 1.
DT   25-MAY-2022, entry version 84.
DE   RecName: Full=Cyanate hydratase {ECO:0000255|HAMAP-Rule:MF_03139};
DE            Short=Cyanase {ECO:0000255|HAMAP-Rule:MF_03139};
DE            EC=4.2.1.104 {ECO:0000255|HAMAP-Rule:MF_03139};
DE   AltName: Full=Cyanate hydrolase {ECO:0000255|HAMAP-Rule:MF_03139};
DE   AltName: Full=Cyanate lyase {ECO:0000255|HAMAP-Rule:MF_03139};
GN   Name=CYN {ECO:0000255|HAMAP-Rule:MF_03139};
OS   Zea mays (Maize).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; PACMAD clade;
OC   Panicoideae; Andropogonodae; Andropogoneae; Tripsacinae; Zea.
OX   NCBI_TaxID=4577;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RX   PubMed=18937034; DOI=10.1007/s11103-008-9415-4;
RA   Alexandrov N.N., Brover V.V., Freidin S., Troukhan M.E., Tatarinova T.V.,
RA   Zhang H., Swaller T.J., Lu Y.-P., Bouck J., Flavell R.B., Feldmann K.A.;
RT   "Insights into corn genes derived from large-scale cDNA sequencing.";
RL   Plant Mol. Biol. 69:179-194(2009).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
RC   STRAIN=B73;
RA   Yu Y., Currie J., Lomeli R., Angelova A., Collura K., Wissotski M.,
RA   Campos D., Kudrna D., Golser W., Ashely E., Descour A., Fernandes J.,
RA   Soderlund C., Walbot V.;
RT   "Maize full-length cDNA project.";
RL   Submitted (MAY-2009) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Catalyzes the reaction of cyanate with bicarbonate to produce
CC       ammonia and carbon dioxide. {ECO:0000255|HAMAP-Rule:MF_03139}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=cyanate + 3 H(+) + hydrogencarbonate = 2 CO2 + NH4(+);
CC         Xref=Rhea:RHEA:11120, ChEBI:CHEBI:15378, ChEBI:CHEBI:16526,
CC         ChEBI:CHEBI:17544, ChEBI:CHEBI:28938, ChEBI:CHEBI:29195;
CC         EC=4.2.1.104; Evidence={ECO:0000255|HAMAP-Rule:MF_03139};
CC   -!- ALTERNATIVE PRODUCTS:
CC       Event=Alternative splicing; Named isoforms=2;
CC       Name=1;
CC         IsoId=B6TTW1-1; Sequence=Displayed;
CC       Name=2;
CC         IsoId=B6TTW1-2; Sequence=VSP_040365, VSP_040366;
CC   -!- SIMILARITY: Belongs to the cyanase family. {ECO:0000255|HAMAP-
CC       Rule:MF_03139}.
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DR   EMBL; EU968426; ACG40544.1; -; mRNA.
DR   EMBL; BT063810; ACN28507.1; -; mRNA.
DR   EMBL; BT084987; ACR35340.1; -; mRNA.
DR   RefSeq; NP_001150815.1; NM_001157343.2. [B6TTW1-1]
DR   AlphaFoldDB; B6TTW1; -.
DR   SMR; B6TTW1; -.
DR   STRING; 4577.GRMZM2G134747_P01; -.
DR   PaxDb; B6TTW1; -.
DR   EnsemblPlants; Zm00001eb046120_T002; Zm00001eb046120_P002; Zm00001eb046120. [B6TTW1-1]
DR   GeneID; 100284448; -.
DR   Gramene; Zm00001eb046120_T002; Zm00001eb046120_P002; Zm00001eb046120. [B6TTW1-1]
DR   KEGG; zma:100284448; -.
DR   eggNOG; ENOG502RY7W; Eukaryota.
DR   HOGENOM; CLU_103452_2_0_1; -.
DR   OMA; YELVMIN; -.
DR   Proteomes; UP000007305; Chromosome 1.
DR   ExpressionAtlas; B6TTW1; baseline and differential.
DR   GO; GO:0008824; F:cyanate hydratase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0003677; F:DNA binding; IEA:InterPro.
DR   GO; GO:0042802; F:identical protein binding; IEA:EnsemblPlants.
DR   GO; GO:0009440; P:cyanate catabolic process; IEA:EnsemblPlants.
DR   GO; GO:0009651; P:response to salt stress; IEA:EnsemblPlants.
DR   CDD; cd00559; Cyanase_C; 1.
DR   Gene3D; 1.10.260.40; -; 1.
DR   Gene3D; 3.30.1160.10; -; 1.
DR   HAMAP; MF_00535; Cyanate_hydrat; 1.
DR   InterPro; IPR008076; Cyanase.
DR   InterPro; IPR003712; Cyanate_lyase_C.
DR   InterPro; IPR036581; Cyanate_lyase_C_sf.
DR   InterPro; IPR010982; Lambda_DNA-bd_dom_sf.
DR   PANTHER; PTHR34186; PTHR34186; 1.
DR   Pfam; PF02560; Cyanate_lyase; 1.
DR   PIRSF; PIRSF001263; Cyanate_hydratas; 1.
DR   PRINTS; PR01693; CYANASE.
DR   SMART; SM01116; Cyanate_lyase; 1.
DR   SUPFAM; SSF47413; SSF47413; 1.
DR   SUPFAM; SSF55234; SSF55234; 1.
DR   TIGRFAMs; TIGR00673; cynS; 1.
PE   2: Evidence at transcript level;
KW   Alternative splicing; Lyase; Reference proteome.
FT   CHAIN           1..166
FT                   /note="Cyanate hydratase"
FT                   /id="PRO_0000403231"
FT   ACT_SITE        92
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_03139"
FT   ACT_SITE        95
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_03139"
FT   ACT_SITE        118
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_03139"
FT   VAR_SEQ         116
FT                   /note="I -> M (in isoform 2)"
FT                   /evidence="ECO:0000303|Ref.2"
FT                   /id="VSP_040365"
FT   VAR_SEQ         117..166
FT                   /note="Missing (in isoform 2)"
FT                   /evidence="ECO:0000303|Ref.2"
FT                   /id="VSP_040366"
SQ   SEQUENCE   166 AA;  18526 MW;  4030DBD0411987E5 CRC64;
     MEASGEKAAV VRRLMEAKEV SGKTFSGIAA ETGLTNVYVA QLLRRQAQLK ADTVPALRAA
     LPTLTDDLIE LMMQPPFRSY HPNIVHEPAI YRLNEAVMHF GESIKEIINE EFGDGIMSAI
     DFYCSVDKVE GADGKDRVVV TFDGKYLPYT EQKSEHMMSR PTRKTS