CYNS_NOSS1
ID CYNS_NOSS1 Reviewed; 146 AA.
AC P58703;
DT 31-JAN-2002, integrated into UniProtKB/Swiss-Prot.
DT 31-JAN-2002, sequence version 1.
DT 25-MAY-2022, entry version 102.
DE RecName: Full=Cyanate hydratase {ECO:0000255|HAMAP-Rule:MF_00535};
DE Short=Cyanase {ECO:0000255|HAMAP-Rule:MF_00535};
DE EC=4.2.1.104 {ECO:0000255|HAMAP-Rule:MF_00535};
DE AltName: Full=Cyanate hydrolase {ECO:0000255|HAMAP-Rule:MF_00535};
DE AltName: Full=Cyanate lyase {ECO:0000255|HAMAP-Rule:MF_00535};
GN Name=cynS {ECO:0000255|HAMAP-Rule:MF_00535}; OrderedLocusNames=all1291;
OS Nostoc sp. (strain PCC 7120 / SAG 25.82 / UTEX 2576).
OC Bacteria; Cyanobacteria; Nostocales; Nostocaceae; Nostoc.
OX NCBI_TaxID=103690;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=PCC 7120 / SAG 25.82 / UTEX 2576;
RX PubMed=11759840; DOI=10.1093/dnares/8.5.205;
RA Kaneko T., Nakamura Y., Wolk C.P., Kuritz T., Sasamoto S., Watanabe A.,
RA Iriguchi M., Ishikawa A., Kawashima K., Kimura T., Kishida Y., Kohara M.,
RA Matsumoto M., Matsuno A., Muraki A., Nakazaki N., Shimpo S., Sugimoto M.,
RA Takazawa M., Yamada M., Yasuda M., Tabata S.;
RT "Complete genomic sequence of the filamentous nitrogen-fixing
RT cyanobacterium Anabaena sp. strain PCC 7120.";
RL DNA Res. 8:205-213(2001).
CC -!- FUNCTION: Catalyzes the reaction of cyanate with bicarbonate to produce
CC ammonia and carbon dioxide. {ECO:0000255|HAMAP-Rule:MF_00535}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=cyanate + 3 H(+) + hydrogencarbonate = 2 CO2 + NH4(+);
CC Xref=Rhea:RHEA:11120, ChEBI:CHEBI:15378, ChEBI:CHEBI:16526,
CC ChEBI:CHEBI:17544, ChEBI:CHEBI:28938, ChEBI:CHEBI:29195;
CC EC=4.2.1.104; Evidence={ECO:0000255|HAMAP-Rule:MF_00535};
CC -!- SIMILARITY: Belongs to the cyanase family. {ECO:0000255|HAMAP-
CC Rule:MF_00535}.
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DR EMBL; BA000019; BAB73248.1; -; Genomic_DNA.
DR PIR; AH1967; AH1967.
DR RefSeq; WP_010995463.1; NZ_RSCN01000021.1.
DR AlphaFoldDB; P58703; -.
DR SMR; P58703; -.
DR STRING; 103690.17130638; -.
DR EnsemblBacteria; BAB73248; BAB73248; BAB73248.
DR KEGG; ana:all1291; -.
DR eggNOG; COG1513; Bacteria.
DR OMA; YELVMIN; -.
DR OrthoDB; 1506439at2; -.
DR Proteomes; UP000002483; Chromosome.
DR GO; GO:0008824; F:cyanate hydratase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0003677; F:DNA binding; IEA:InterPro.
DR GO; GO:0009439; P:cyanate metabolic process; IEA:UniProtKB-UniRule.
DR CDD; cd00559; Cyanase_C; 1.
DR Gene3D; 1.10.260.40; -; 1.
DR Gene3D; 3.30.1160.10; -; 1.
DR HAMAP; MF_00535; Cyanate_hydrat; 1.
DR InterPro; IPR008076; Cyanase.
DR InterPro; IPR003712; Cyanate_lyase_C.
DR InterPro; IPR036581; Cyanate_lyase_C_sf.
DR InterPro; IPR010982; Lambda_DNA-bd_dom_sf.
DR PANTHER; PTHR34186; PTHR34186; 1.
DR Pfam; PF02560; Cyanate_lyase; 1.
DR PIRSF; PIRSF001263; Cyanate_hydratas; 1.
DR PRINTS; PR01693; CYANASE.
DR SMART; SM01116; Cyanate_lyase; 1.
DR SUPFAM; SSF47413; SSF47413; 1.
DR SUPFAM; SSF55234; SSF55234; 1.
DR TIGRFAMs; TIGR00673; cynS; 1.
PE 3: Inferred from homology;
KW Lyase; Reference proteome.
FT CHAIN 1..146
FT /note="Cyanate hydratase"
FT /id="PRO_0000187520"
FT ACT_SITE 87
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00535"
FT ACT_SITE 90
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00535"
FT ACT_SITE 113
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00535"
SQ SEQUENCE 146 AA; 16399 MW; 6EEA2F0D9C71E694 CRC64;
MSIPEITQTL LQAKKDKGLS FADLEATLGR DEVCIAALFY RQASASEEEA KLLVEALGLD
SSYIKHLTEY PVKGLGPVVP TDPLIYRFYE IMQVYGFPIK EVIQEKFGDG IMSAIDFTLD
VEKEADPKGD RVKITMSGKF LPYKKW
