ID   CYNS_SERP5              Reviewed;         156 AA.
AC   A8GBZ7;
DT   05-FEB-2008, integrated into UniProtKB/Swiss-Prot.
DT   13-NOV-2007, sequence version 1.
DT   25-MAY-2022, entry version 79.
DE   RecName: Full=Cyanate hydratase {ECO:0000255|HAMAP-Rule:MF_00535};
DE            Short=Cyanase {ECO:0000255|HAMAP-Rule:MF_00535};
DE            EC=4.2.1.104 {ECO:0000255|HAMAP-Rule:MF_00535};
DE   AltName: Full=Cyanate hydrolase {ECO:0000255|HAMAP-Rule:MF_00535};
DE   AltName: Full=Cyanate lyase {ECO:0000255|HAMAP-Rule:MF_00535};
GN   Name=cynS {ECO:0000255|HAMAP-Rule:MF_00535}; OrderedLocusNames=Spro_1533;
OS   Serratia proteamaculans (strain 568).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Yersiniaceae; Serratia.
OX   NCBI_TaxID=399741;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=568;
RG   US DOE Joint Genome Institute;
RA   Copeland A., Lucas S., Lapidus A., Barry K., Glavina del Rio T., Dalin E.,
RA   Tice H., Pitluck S., Chain P., Malfatti S., Shin M., Vergez L., Schmutz J.,
RA   Larimer F., Land M., Hauser L., Kyrpides N., Kim E., Taghavi S., Newman L.,
RA   Vangronsveld J., van der Lelie D., Richardson P.;
RT   "Complete sequence of chromosome of Serratia proteamaculans 568.";
RL   Submitted (SEP-2007) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Catalyzes the reaction of cyanate with bicarbonate to produce
CC       ammonia and carbon dioxide. {ECO:0000255|HAMAP-Rule:MF_00535}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=cyanate + 3 H(+) + hydrogencarbonate = 2 CO2 + NH4(+);
CC         Xref=Rhea:RHEA:11120, ChEBI:CHEBI:15378, ChEBI:CHEBI:16526,
CC         ChEBI:CHEBI:17544, ChEBI:CHEBI:28938, ChEBI:CHEBI:29195;
CC         EC=4.2.1.104; Evidence={ECO:0000255|HAMAP-Rule:MF_00535};
CC   -!- SIMILARITY: Belongs to the cyanase family. {ECO:0000255|HAMAP-
CC       Rule:MF_00535}.
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DR   EMBL; CP000826; ABV40637.1; -; Genomic_DNA.
DR   RefSeq; WP_012005966.1; NC_009832.1.
DR   PDB; 4Y42; X-ray; 2.09 A; A/B/C/D/E/F/G/H/I/J=1-156.
DR   PDB; 6B6M; X-ray; 1.91 A; A/B/C/D/E=1-156.
DR   PDBsum; 4Y42; -.
DR   PDBsum; 6B6M; -.
DR   AlphaFoldDB; A8GBZ7; -.
DR   SMR; A8GBZ7; -.
DR   STRING; 399741.Spro_1533; -.
DR   EnsemblBacteria; ABV40637; ABV40637; Spro_1533.
DR   KEGG; spe:Spro_1533; -.
DR   eggNOG; COG1513; Bacteria.
DR   HOGENOM; CLU_103452_1_1_6; -.
DR   OMA; YELVMIN; -.
DR   OrthoDB; 1506439at2; -.
DR   BRENDA; 4.2.1.104; 8756.
DR   GO; GO:0008824; F:cyanate hydratase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0003677; F:DNA binding; IEA:InterPro.
DR   GO; GO:0009439; P:cyanate metabolic process; IEA:UniProtKB-UniRule.
DR   CDD; cd00559; Cyanase_C; 1.
DR   Gene3D; 1.10.260.40; -; 1.
DR   Gene3D; 3.30.1160.10; -; 1.
DR   HAMAP; MF_00535; Cyanate_hydrat; 1.
DR   InterPro; IPR008076; Cyanase.
DR   InterPro; IPR003712; Cyanate_lyase_C.
DR   InterPro; IPR036581; Cyanate_lyase_C_sf.
DR   InterPro; IPR010982; Lambda_DNA-bd_dom_sf.
DR   PANTHER; PTHR34186; PTHR34186; 1.
DR   Pfam; PF02560; Cyanate_lyase; 1.
DR   PIRSF; PIRSF001263; Cyanate_hydratas; 1.
DR   PRINTS; PR01693; CYANASE.
DR   SMART; SM01116; Cyanate_lyase; 1.
DR   SUPFAM; SSF47413; SSF47413; 1.
DR   SUPFAM; SSF55234; SSF55234; 1.
DR   TIGRFAMs; TIGR00673; cynS; 1.
PE   1: Evidence at protein level;
KW   3D-structure; Lyase.
FT   CHAIN           1..156
FT                   /note="Cyanate hydratase"
FT                   /id="PRO_1000061028"
FT   ACT_SITE        96
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00535"
FT   ACT_SITE        99
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00535"
FT   ACT_SITE        122
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00535"
FT   STRAND          6..8
FT                   /evidence="ECO:0007829|PDB:6B6M"
FT   HELIX           9..25
FT                   /evidence="ECO:0007829|PDB:6B6M"
FT   HELIX           29..32
FT                   /evidence="ECO:0007829|PDB:6B6M"
FT   TURN            33..35
FT                   /evidence="ECO:0007829|PDB:6B6M"
FT   STRAND          36..38
FT                   /evidence="ECO:0007829|PDB:4Y42"
FT   HELIX           40..47
FT                   /evidence="ECO:0007829|PDB:6B6M"
FT   HELIX           55..65
FT                   /evidence="ECO:0007829|PDB:6B6M"
FT   HELIX           69..75
FT                   /evidence="ECO:0007829|PDB:6B6M"
FT   HELIX           92..115
FT                   /evidence="ECO:0007829|PDB:6B6M"
FT   STRAND          118..134
FT                   /evidence="ECO:0007829|PDB:6B6M"
FT   STRAND          138..152
FT                   /evidence="ECO:0007829|PDB:6B6M"
SQ   SEQUENCE   156 AA;  17154 MW;  64D7B5E882F5604D CRC64;
     MTQSLHYSSP RETLTDTIMM AKIRKNLTFE AINQGTGLSL AFVTAALLGQ HPLPEQAARV
     VAEKLDLDED AIRLLQTIPL RGSIPGGVPT DPTIYRFYEM VQIYGSTLKA LVHEQFGDGI
     ISAINFKLDI KKVPDPDGGE RAVITLDGKY LPTKPF