ID   CYNS_SYNE7              Reviewed;         146 AA.
AC   Q59948; O08486; O08512; Q31LD5;
DT   01-NOV-1997, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1996, sequence version 1.
DT   03-AUG-2022, entry version 114.
DE   RecName: Full=Cyanate hydratase {ECO:0000255|HAMAP-Rule:MF_00535};
DE            Short=Cyanase {ECO:0000255|HAMAP-Rule:MF_00535};
DE            EC=4.2.1.104 {ECO:0000255|HAMAP-Rule:MF_00535};
DE   AltName: Full=Cyanate hydrolase {ECO:0000255|HAMAP-Rule:MF_00535};
DE   AltName: Full=Cyanate lyase {ECO:0000255|HAMAP-Rule:MF_00535};
GN   Name=cynS {ECO:0000255|HAMAP-Rule:MF_00535};
GN   OrderedLocusNames=Synpcc7942_2104;
OS   Synechococcus elongatus (strain PCC 7942 / FACHB-805) (Anacystis nidulans
OS   R2).
OC   Bacteria; Cyanobacteria; Synechococcales; Synechococcaceae; Synechococcus.
OX   NCBI_TaxID=1140;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RA   Jalali F., Espie G.S.;
RL   Submitted (JUN-1996) to the EMBL/GenBank/DDBJ databases.
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND CHARACTERIZATION.
RX   PubMed=9294430; DOI=10.1128/jb.179.18.5744-5750.1997;
RA   Harano Y., Suzuki I., Maeda S., Kaneko T., Tabata S., Omata T.;
RT   "Identification and nitrogen regulation of the cyanase gene from the
RT   cyanobacteria Synechocystis sp. strain PCC 6803 and Synechococcus sp.
RT   strain PCC 7942.";
RL   J. Bacteriol. 179:5744-5750(1997).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=PCC 7942 / FACHB-805;
RG   US DOE Joint Genome Institute;
RA   Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina T.,
RA   Hammon N., Israni S., Pitluck S., Schmutz J., Larimer F., Land M.,
RA   Kyrpides N., Lykidis A., Richardson P.;
RT   "Complete sequence of chromosome 1 of Synechococcus elongatus PCC 7942.";
RL   Submitted (AUG-2005) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Catalyzes the reaction of cyanate with bicarbonate to produce
CC       ammonia and carbon dioxide.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=cyanate + 3 H(+) + hydrogencarbonate = 2 CO2 + NH4(+);
CC         Xref=Rhea:RHEA:11120, ChEBI:CHEBI:15378, ChEBI:CHEBI:16526,
CC         ChEBI:CHEBI:17544, ChEBI:CHEBI:28938, ChEBI:CHEBI:29195;
CC         EC=4.2.1.104; Evidence={ECO:0000255|HAMAP-Rule:MF_00535};
CC   -!- SIMILARITY: Belongs to the cyanase family. {ECO:0000255|HAMAP-
CC       Rule:MF_00535}.
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DR   EMBL; U59481; AAB02940.1; -; Genomic_DNA.
DR   EMBL; AB000100; BAA19515.1; -; Genomic_DNA.
DR   EMBL; CP000100; ABB58134.1; -; Genomic_DNA.
DR   RefSeq; WP_011244299.1; NC_007604.1.
DR   AlphaFoldDB; Q59948; -.
DR   SMR; Q59948; -.
DR   STRING; 1140.Synpcc7942_2104; -.
DR   PRIDE; Q59948; -.
DR   EnsemblBacteria; ABB58134; ABB58134; Synpcc7942_2104.
DR   KEGG; syf:Synpcc7942_2104; -.
DR   eggNOG; COG1513; Bacteria.
DR   HOGENOM; CLU_103452_1_0_3; -.
DR   OMA; YELVMIN; -.
DR   OrthoDB; 1506439at2; -.
DR   BioCyc; SYNEL:SYNPCC7942_2104-MON; -.
DR   BRENDA; 4.2.1.104; 7781.
DR   GO; GO:0008824; F:cyanate hydratase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0003677; F:DNA binding; IEA:InterPro.
DR   GO; GO:0009439; P:cyanate metabolic process; IEA:UniProtKB-UniRule.
DR   CDD; cd00559; Cyanase_C; 1.
DR   CDD; cd00093; HTH_XRE; 1.
DR   Gene3D; 1.10.260.40; -; 1.
DR   Gene3D; 3.30.1160.10; -; 1.
DR   HAMAP; MF_00535; Cyanate_hydrat; 1.
DR   InterPro; IPR001387; Cro/C1-type_HTH.
DR   InterPro; IPR008076; Cyanase.
DR   InterPro; IPR003712; Cyanate_lyase_C.
DR   InterPro; IPR036581; Cyanate_lyase_C_sf.
DR   InterPro; IPR010982; Lambda_DNA-bd_dom_sf.
DR   PANTHER; PTHR34186; PTHR34186; 1.
DR   Pfam; PF02560; Cyanate_lyase; 1.
DR   PIRSF; PIRSF001263; Cyanate_hydratas; 1.
DR   PRINTS; PR01693; CYANASE.
DR   SMART; SM01116; Cyanate_lyase; 1.
DR   SUPFAM; SSF47413; SSF47413; 1.
DR   SUPFAM; SSF55234; SSF55234; 1.
DR   TIGRFAMs; TIGR00673; cynS; 1.
PE   1: Evidence at protein level;
KW   Lyase.
FT   CHAIN           1..146
FT                   /note="Cyanate hydratase"
FT                   /id="PRO_0000187530"
FT   ACT_SITE        87
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00535"
FT   ACT_SITE        90
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00535"
FT   ACT_SITE        113
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00535"
SQ   SEQUENCE   146 AA;  16362 MW;  CE8714E1F1FDE04E CRC64;
     MTSAITEQLL KAKKAKGITF TELEQLLGRD EVWIASVFYR QSTASPEEAE KLLTALGLDL
     ALADELTTPP VKGCLEPVIP TDPLIYRFYE IMQVYGLPLK DVIQEKFGDG IMSAIDFTLD
     VDKVEDPKGD RVKVTMCGKF LAYKKW