ID   CYNT_PSEAE              Reviewed;         220 AA.
AC   Q9I262;
DT   15-MAY-2007, integrated into UniProtKB/Swiss-Prot.
DT   01-MAR-2001, sequence version 1.
DT   03-AUG-2022, entry version 115.
DE   RecName: Full=Carbonic anhydrase;
DE            EC=4.2.1.1;
DE   AltName: Full=Carbonate dehydratase;
GN   Name=cynT; OrderedLocusNames=PA2053;
OS   Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM
OS   14847 / LMG 12228 / 1C / PRS 101 / PAO1).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales;
OC   Pseudomonadaceae; Pseudomonas.
OX   NCBI_TaxID=208964;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C /
RC   PRS 101 / PAO1;
RX   PubMed=10984043; DOI=10.1038/35023079;
RA   Stover C.K., Pham X.-Q.T., Erwin A.L., Mizoguchi S.D., Warrener P.,
RA   Hickey M.J., Brinkman F.S.L., Hufnagle W.O., Kowalik D.J., Lagrou M.,
RA   Garber R.L., Goltry L., Tolentino E., Westbrock-Wadman S., Yuan Y.,
RA   Brody L.L., Coulter S.N., Folger K.R., Kas A., Larbig K., Lim R.M.,
RA   Smith K.A., Spencer D.H., Wong G.K.-S., Wu Z., Paulsen I.T., Reizer J.,
RA   Saier M.H. Jr., Hancock R.E.W., Lory S., Olson M.V.;
RT   "Complete genome sequence of Pseudomonas aeruginosa PAO1, an opportunistic
RT   pathogen.";
RL   Nature 406:959-964(2000).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=H(+) + hydrogencarbonate = CO2 + H2O; Xref=Rhea:RHEA:10748,
CC         ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:16526,
CC         ChEBI:CHEBI:17544; EC=4.2.1.1;
CC   -!- COFACTOR:
CC       Name=Zn(2+); Xref=ChEBI:CHEBI:29105; Evidence={ECO:0000250};
CC       Note=Binds 1 zinc ion per subunit. {ECO:0000250};
CC   -!- SIMILARITY: Belongs to the beta-class carbonic anhydrase family.
CC       {ECO:0000305}.
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DR   EMBL; AE004091; AAG05441.1; -; Genomic_DNA.
DR   PIR; D83390; D83390.
DR   RefSeq; NP_250743.1; NC_002516.2.
DR   RefSeq; WP_003107081.1; NZ_QZGE01000022.1.
DR   AlphaFoldDB; Q9I262; -.
DR   SMR; Q9I262; -.
DR   STRING; 287.DR97_5793; -.
DR   PaxDb; Q9I262; -.
DR   PRIDE; Q9I262; -.
DR   DNASU; 878074; -.
DR   EnsemblBacteria; AAG05441; AAG05441; PA2053.
DR   GeneID; 878074; -.
DR   KEGG; pae:PA2053; -.
DR   PATRIC; fig|208964.12.peg.2141; -.
DR   PseudoCAP; PA2053; -.
DR   HOGENOM; CLU_053879_5_3_6; -.
DR   InParanoid; Q9I262; -.
DR   OMA; ANIRTHP; -.
DR   PhylomeDB; Q9I262; -.
DR   BioCyc; PAER208964:G1FZ6-2091-MON; -.
DR   Proteomes; UP000002438; Chromosome.
DR   GO; GO:0004089; F:carbonate dehydratase activity; IDA:PseudoCAP.
DR   GO; GO:0008270; F:zinc ion binding; IDA:PseudoCAP.
DR   GO; GO:0015976; P:carbon utilization; IEA:InterPro.
DR   GO; GO:0009440; P:cyanate catabolic process; IBA:GO_Central.
DR   CDD; cd00884; beta_CA_cladeB; 1.
DR   Gene3D; 3.40.1050.10; -; 1.
DR   InterPro; IPR045066; Beta_CA_cladeB.
DR   InterPro; IPR001765; Carbonic_anhydrase.
DR   InterPro; IPR015892; Carbonic_anhydrase_CS.
DR   InterPro; IPR036874; Carbonic_anhydrase_sf.
DR   PANTHER; PTHR11002; PTHR11002; 1.
DR   Pfam; PF00484; Pro_CA; 1.
DR   SMART; SM00947; Pro_CA; 1.
DR   SUPFAM; SSF53056; SSF53056; 1.
DR   PROSITE; PS00704; PROK_CO2_ANHYDRASE_1; 1.
DR   PROSITE; PS00705; PROK_CO2_ANHYDRASE_2; 1.
PE   3: Inferred from homology;
KW   Lyase; Metal-binding; Reference proteome; Zinc.
FT   CHAIN           1..220
FT                   /note="Carbonic anhydrase"
FT                   /id="PRO_0000287738"
FT   BINDING         39
FT                   /ligand="Zn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29105"
FT                   /evidence="ECO:0000250"
FT   BINDING         41
FT                   /ligand="Zn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29105"
FT                   /evidence="ECO:0000250"
FT   BINDING         98
FT                   /ligand="Zn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29105"
FT                   /evidence="ECO:0000250"
FT   BINDING         101
FT                   /ligand="Zn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29105"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   220 AA;  23404 MW;  8B09AC4B1B76CF23 CRC64;
     MRDIIDGFLR FQRDAYPARS QLFKSLATRQ APKALFIACS DSRVVPELLT QREPGELFVI
     RNAGNIVPGY GPQPGGVSAS VEYAVAVLGV GDIVVCGHSD CGAMGAIASC ACLDQLPAVA
     GWLHHAEAAR AMNSAHEHAS EAARLDALVR HNVIAQLANL RTHPCVARAL EQGRLNLHGW
     VYDIESGRID ALDGASRRFV SLAEHPEVRA VGGEPGQAVA