CYO12_VIOAR
ID CYO12_VIOAR Reviewed; 29 AA.
AC P83835; P58444; P58450;
DT 26-APR-2004, integrated into UniProtKB/Swiss-Prot.
DT 26-APR-2004, sequence version 1.
DT 25-MAY-2022, entry version 46.
DE RecName: Full=Varv peptide E;
OS Viola arvensis (European field pansy) (Field violet).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; fabids; Malpighiales; Violaceae; Viola.
OX NCBI_TaxID=97415;
RN [1]
RP PROTEIN SEQUENCE, AND MASS SPECTROMETRY.
RX PubMed=10075760; DOI=10.1021/np9803878;
RA Goeransson U., Luijendijk T., Johansson S., Bohlin L., Claeson P.;
RT "Seven novel macrocyclic polypeptides from Viola arvensis.";
RL J. Nat. Prod. 62:283-286(1999).
RN [2]
RP PROTEIN SEQUENCE, FUNCTION, AND MASS SPECTROMETRY.
RX PubMed=14987049; DOI=10.1021/np030101l;
RA Svangard E., Goransson U., Hocaoglu Z., Gullbo J., Larsson R., Claeson P.,
RA Bohlin L.;
RT "Cytotoxic cyclotides from Viola tricolor.";
RL J. Nat. Prod. 67:144-147(2004).
CC -!- FUNCTION: Probably participates in a plant defense mechanism. Has
CC cytotoxic activity against human lymphoma U-937 GTB and human myeloma
CC RPMI-8226/s cell lines. {ECO:0000255|PROSITE-ProRule:PRU00395,
CC ECO:0000269|PubMed:14987049}.
CC -!- DOMAIN: The presence of a 'disulfide through disulfide knot'
CC structurally defines this protein as a knottin.
CC -!- PTM: This is a cyclic peptide.
CC -!- MASS SPECTROMETRY: Mass=2894.9; Mass_error=3; Method=MALDI;
CC Evidence={ECO:0000269|PubMed:10075760};
CC -!- MASS SPECTROMETRY: Mass=2892.2; Method=Electrospray;
CC Evidence={ECO:0000269|PubMed:14987049};
CC -!- SIMILARITY: Belongs to the cyclotide family. Moebius subfamily.
CC {ECO:0000255|PROSITE-ProRule:PRU00395}.
CC -!- CAUTION: This peptide is cyclic. The start position was chosen by
CC similarity to OAK1 (kalata-B1) for which the DNA sequence is known.
CC {ECO:0000305}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR AlphaFoldDB; P83835; -.
DR SMR; P83835; -.
DR GO; GO:0006952; P:defense response; IDA:UniProtKB.
DR InterPro; IPR005535; Cyclotide.
DR InterPro; IPR012324; Cyclotide_moebius_CS.
DR InterPro; IPR036146; Cyclotide_sf.
DR Pfam; PF03784; Cyclotide; 1.
DR PIRSF; PIRSF037891; Cycloviolacin; 1.
DR SUPFAM; SSF57038; SSF57038; 1.
DR PROSITE; PS51052; CYCLOTIDE; 1.
DR PROSITE; PS60009; CYCLOTIDE_MOEBIUS; 1.
PE 1: Evidence at protein level;
KW Direct protein sequencing; Disulfide bond; Knottin; Plant defense.
FT PEPTIDE 1..29
FT /note="Varv peptide E"
FT /id="PRO_0000043618"
FT DISULFID 5..19
FT DISULFID 9..21
FT DISULFID 14..26
FT CROSSLNK 1..29
FT /note="Cyclopeptide (Gly-Asn)"
SQ SEQUENCE 29 AA; 2916 MW; 323641013F82FA18 CRC64;
GLPICGETCV GGTCNTPGCS CSWPVCTRN