ID   CYOA_BUCBP              Reviewed;         286 AA.
AC   Q89AA3;
DT   13-APR-2004, integrated into UniProtKB/Swiss-Prot.
DT   13-APR-2004, sequence version 2.
DT   03-AUG-2022, entry version 117.
DE   RecName: Full=Cytochrome bo(3) ubiquinol oxidase subunit 2;
DE   AltName: Full=Cytochrome o ubiquinol oxidase subunit 2;
DE            Short=Cytochrome o subunit 2;
DE   AltName: Full=Oxidase bo(3) subunit 2;
DE   AltName: Full=Ubiquinol oxidase polypeptide II;
DE   AltName: Full=Ubiquinol oxidase subunit 2;
DE   Flags: Precursor;
GN   Name=cyoA; OrderedLocusNames=bbp_417;
OS   Buchnera aphidicola subsp. Baizongia pistaciae (strain Bp).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Erwiniaceae; Buchnera.
OX   NCBI_TaxID=224915;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=Bp;
RX   PubMed=12522265; DOI=10.1073/pnas.0235981100;
RA   van Ham R.C.H.J., Kamerbeek J., Palacios C., Rausell C., Abascal F.,
RA   Bastolla U., Fernandez J.M., Jimenez L., Postigo M., Silva F.J.,
RA   Tamames J., Viguera E., Latorre A., Valencia A., Moran F., Moya A.;
RT   "Reductive genome evolution in Buchnera aphidicola.";
RL   Proc. Natl. Acad. Sci. U.S.A. 100:581-586(2003).
CC   -!- FUNCTION: Cytochrome bo(3) ubiquinol terminal oxidase is the component
CC       of the aerobic respiratory chain of E.coli that predominates when cells
CC       are grown at high aeration. Has proton pump activity across the
CC       membrane in addition to electron transfer, pumping 2 protons/electron
CC       (By similarity). {ECO:0000250}.
CC   -!- SUBUNIT: Heterooctamer of two A chains, two B chains, two C chains and
CC       two D chains. {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000250}; Multi-pass membrane
CC       protein {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the cytochrome c oxidase subunit 2 family.
CC       {ECO:0000305}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=AAO27127.1; Type=Erroneous initiation; Note=Extended N-terminus.; Evidence={ECO:0000305};
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DR   EMBL; AE016826; AAO27127.1; ALT_INIT; Genomic_DNA.
DR   RefSeq; WP_044010653.1; NC_004545.1.
DR   AlphaFoldDB; Q89AA3; -.
DR   SMR; Q89AA3; -.
DR   STRING; 224915.bbp_417; -.
DR   EnsemblBacteria; AAO27127; AAO27127; bbp_417.
DR   GeneID; 56470952; -.
DR   KEGG; bab:bbp_417; -.
DR   eggNOG; COG1622; Bacteria.
DR   HOGENOM; CLU_036876_6_1_6; -.
DR   OMA; TSLDWKW; -.
DR   OrthoDB; 1654242at2; -.
DR   Proteomes; UP000000601; Chromosome.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0070469; C:respirasome; IEA:UniProtKB-KW.
DR   GO; GO:0005507; F:copper ion binding; IEA:InterPro.
DR   GO; GO:0009486; F:cytochrome bo3 ubiquinol oxidase activity; IEA:InterPro.
DR   GO; GO:0004129; F:cytochrome-c oxidase activity; IEA:InterPro.
DR   GO; GO:0016682; F:oxidoreductase activity, acting on diphenols and related substances as donors, oxygen as acceptor; IEA:InterPro.
DR   CDD; cd04212; CuRO_UO_II; 1.
DR   Gene3D; 1.10.287.90; -; 1.
DR   Gene3D; 2.60.40.420; -; 1.
DR   InterPro; IPR045187; CcO_II.
DR   InterPro; IPR002429; CcO_II-like_C.
DR   InterPro; IPR010514; COX_ARM.
DR   InterPro; IPR008972; Cupredoxin.
DR   InterPro; IPR034227; CuRO_UO_II.
DR   InterPro; IPR011759; Cyt_c_oxidase_su2_TM_dom.
DR   InterPro; IPR036257; Cyt_c_oxidase_su2_TM_sf.
DR   InterPro; IPR006333; Cyt_o_ubiquinol_oxidase_su2.
DR   PANTHER; PTHR22888; PTHR22888; 1.
DR   Pfam; PF00116; COX2; 1.
DR   Pfam; PF06481; COX_ARM; 1.
DR   PIRSF; PIRSF000292; Ubi_od_II; 1.
DR   SUPFAM; SSF49503; SSF49503; 1.
DR   SUPFAM; SSF81464; SSF81464; 1.
DR   TIGRFAMs; TIGR01433; CyoA; 1.
DR   PROSITE; PS50857; COX2_CUA; 1.
DR   PROSITE; PS50999; COX2_TM; 1.
PE   3: Inferred from homology;
KW   Cell membrane; Electron transport; Lipoprotein; Membrane; Oxidoreductase;
KW   Palmitate; Reference proteome; Respiratory chain; Signal; Transmembrane;
KW   Transmembrane helix; Transport.
FT   SIGNAL          1..24
FT                   /evidence="ECO:0000255"
FT   CHAIN           25..286
FT                   /note="Cytochrome bo(3) ubiquinol oxidase subunit 2"
FT                   /id="PRO_0000006074"
FT   TOPO_DOM        25..44
FT                   /note="Extracellular"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        45..67
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        68..85
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        86..108
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        109..286
FT                   /note="Extracellular"
FT                   /evidence="ECO:0000255"
FT   LIPID           25
FT                   /note="N-palmitoyl cysteine"
FT                   /evidence="ECO:0000255"
FT   LIPID           25
FT                   /note="S-diacylglycerol cysteine"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   286 AA;  33106 MW;  8B2397BB39E88CF0 CRC64;
     MQFIKYKSYI LKFLLVSCIF CINGCDCTIL CPNGLIAQEQ RFVLFVSFFT MLLIIIPVIF
     MTIFFVLRYR ESNFSKTYDP KWSHSNIIEL LIWGIPIIII VFLSIFSWKS VHDLDPKKPI
     VSNVQPIKIN VISLDWKWLF IYPDQKIATI NKLIIPINTP IIFNLTSGSV MNSFFIPSLG
     SQIYVMPGMK TNLNLIANKL GQFKGFSSNY SGKGFSNMKF DVLVTSDHIF FYEWVKKIQK
     SKYKLNSMYQ FNQLAIPSDN NAIKYFSNLK ENLFNVVIAN VLKISL