CYOA_PSEAE
ID CYOA_PSEAE Reviewed; 331 AA.
AC Q9I427;
DT 15-MAY-2007, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2001, sequence version 1.
DT 03-AUG-2022, entry version 125.
DE RecName: Full=Cytochrome bo(3) ubiquinol oxidase subunit 2;
DE AltName: Full=Cytochrome o ubiquinol oxidase subunit 2;
DE Short=Cytochrome o subunit 2;
DE AltName: Full=Oxidase bo(3) subunit 2;
DE AltName: Full=Ubiquinol oxidase polypeptide II;
DE AltName: Full=Ubiquinol oxidase subunit 2;
DE Flags: Precursor;
GN Name=cyoA; OrderedLocusNames=PA1317;
OS Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM
OS 14847 / LMG 12228 / 1C / PRS 101 / PAO1).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales;
OC Pseudomonadaceae; Pseudomonas.
OX NCBI_TaxID=208964;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C /
RC PRS 101 / PAO1;
RX PubMed=10984043; DOI=10.1038/35023079;
RA Stover C.K., Pham X.-Q.T., Erwin A.L., Mizoguchi S.D., Warrener P.,
RA Hickey M.J., Brinkman F.S.L., Hufnagle W.O., Kowalik D.J., Lagrou M.,
RA Garber R.L., Goltry L., Tolentino E., Westbrock-Wadman S., Yuan Y.,
RA Brody L.L., Coulter S.N., Folger K.R., Kas A., Larbig K., Lim R.M.,
RA Smith K.A., Spencer D.H., Wong G.K.-S., Wu Z., Paulsen I.T., Reizer J.,
RA Saier M.H. Jr., Hancock R.E.W., Lory S., Olson M.V.;
RT "Complete genome sequence of Pseudomonas aeruginosa PAO1, an opportunistic
RT pathogen.";
RL Nature 406:959-964(2000).
CC -!- FUNCTION: Cytochrome bo(3) ubiquinol terminal oxidase is the component
CC of the aerobic respiratory chain of E.coli that predominates when cells
CC are grown at high aeration. Has proton pump activity across the
CC membrane in addition to electron transfer, pumping 2 protons/electron
CC (By similarity). {ECO:0000250}.
CC -!- SUBUNIT: Heterooctamer of two A chains, two B chains, two C chains and
CC two D chains. {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Cell inner membrane {ECO:0000250}; Multi-pass
CC membrane protein {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the cytochrome c oxidase subunit 2 family.
CC {ECO:0000305}.
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DR EMBL; AE004091; AAG04706.1; -; Genomic_DNA.
DR PIR; D83480; D83480.
DR RefSeq; NP_250008.1; NC_002516.2.
DR RefSeq; WP_003082702.1; NZ_QZGE01000005.1.
DR AlphaFoldDB; Q9I427; -.
DR SMR; Q9I427; -.
DR STRING; 287.DR97_477; -.
DR PaxDb; Q9I427; -.
DR PRIDE; Q9I427; -.
DR DNASU; 881513; -.
DR EnsemblBacteria; AAG04706; AAG04706; PA1317.
DR GeneID; 881513; -.
DR KEGG; pae:PA1317; -.
DR PATRIC; fig|208964.12.peg.1369; -.
DR PseudoCAP; PA1317; -.
DR HOGENOM; CLU_036876_6_1_6; -.
DR InParanoid; Q9I427; -.
DR OMA; TAMNSFF; -.
DR PhylomeDB; Q9I427; -.
DR BioCyc; PAER208964:G1FZ6-1342-MON; -.
DR Proteomes; UP000002438; Chromosome.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0070469; C:respirasome; IEA:UniProtKB-KW.
DR GO; GO:0005507; F:copper ion binding; IEA:InterPro.
DR GO; GO:0009486; F:cytochrome bo3 ubiquinol oxidase activity; IEA:InterPro.
DR GO; GO:0004129; F:cytochrome-c oxidase activity; IEA:InterPro.
DR GO; GO:0016682; F:oxidoreductase activity, acting on diphenols and related substances as donors, oxygen as acceptor; IEA:InterPro.
DR GO; GO:0042773; P:ATP synthesis coupled electron transport; IBA:GO_Central.
DR CDD; cd04212; CuRO_UO_II; 1.
DR Gene3D; 1.10.287.90; -; 1.
DR Gene3D; 2.60.40.420; -; 1.
DR InterPro; IPR045187; CcO_II.
DR InterPro; IPR002429; CcO_II-like_C.
DR InterPro; IPR010514; COX_ARM.
DR InterPro; IPR008972; Cupredoxin.
DR InterPro; IPR034227; CuRO_UO_II.
DR InterPro; IPR011759; Cyt_c_oxidase_su2_TM_dom.
DR InterPro; IPR036257; Cyt_c_oxidase_su2_TM_sf.
DR InterPro; IPR006333; Cyt_o_ubiquinol_oxidase_su2.
DR PANTHER; PTHR22888; PTHR22888; 1.
DR Pfam; PF00116; COX2; 1.
DR Pfam; PF06481; COX_ARM; 1.
DR PIRSF; PIRSF000292; Ubi_od_II; 1.
DR SUPFAM; SSF49503; SSF49503; 1.
DR SUPFAM; SSF81464; SSF81464; 1.
DR TIGRFAMs; TIGR01433; CyoA; 1.
DR PROSITE; PS50857; COX2_CUA; 1.
DR PROSITE; PS50999; COX2_TM; 1.
DR PROSITE; PS51257; PROKAR_LIPOPROTEIN; 1.
PE 3: Inferred from homology;
KW Cell inner membrane; Cell membrane; Electron transport; Lipoprotein;
KW Membrane; Oxidoreductase; Palmitate; Reference proteome; Respiratory chain;
KW Signal; Transmembrane; Transmembrane helix; Transport.
FT SIGNAL 1..23
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00303"
FT CHAIN 24..331
FT /note="Cytochrome bo(3) ubiquinol oxidase subunit 2"
FT /id="PRO_0000287756"
FT TOPO_DOM 24..41
FT /note="Periplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 42..62
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 63..86
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 87..107
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 108..331
FT /note="Periplasmic"
FT /evidence="ECO:0000255"
FT LIPID 24
FT /note="N-palmitoyl cysteine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00303"
FT LIPID 24
FT /note="S-diacylglycerol cysteine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00303"
SQ SEQUENCE 331 AA; 36623 MW; DC4449ED0FF0BB66 CRC64;
MTKANPFAAL KWLSLAPALL LGGCDMTLFN PKGQVGMDER TLIITATLLM LIVVIPVIVM
TLAFAWKYRA SNTQAEYKPD WHHSNRIEAV VWLVPCVIIA ILGWITWEST HKLDPYRPLD
SEVKPVTIQA VSLDWKWLFI YPEQGIATVN EIAFPKDTPV NFQITSDSVM NSFFIPQLGS
QIYSMAGMMT KLHLIANEEG VFDGISANYS GGGFSGMRFK AIATSEQGFQ DWVAKVKAAP
ASLSIGTYPE LVKPSENVPP TYFSSVSPEL FGHILTKYEH HGDAKGAAHG EHAGAEHEAA
MTGHDMQDMD MQAMQGMKDM KDMHMQPSTQ E
