ID   CYOD_PSEAE              Reviewed;         111 AA.
AC   Q9I424;
DT   15-MAY-2007, integrated into UniProtKB/Swiss-Prot.
DT   01-MAR-2001, sequence version 1.
DT   03-AUG-2022, entry version 98.
DE   RecName: Full=Cytochrome bo(3) ubiquinol oxidase subunit 4;
DE   AltName: Full=Cytochrome o ubiquinol oxidase subunit 4;
DE            Short=Cytochrome o subunit 4;
DE   AltName: Full=Oxidase bo(3) subunit 4;
DE   AltName: Full=Ubiquinol oxidase polypeptide IV;
DE   AltName: Full=Ubiquinol oxidase subunit 4;
GN   Name=cyoD; OrderedLocusNames=PA1320;
OS   Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM
OS   14847 / LMG 12228 / 1C / PRS 101 / PAO1).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales;
OC   Pseudomonadaceae; Pseudomonas.
OX   NCBI_TaxID=208964;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C /
RC   PRS 101 / PAO1;
RX   PubMed=10984043; DOI=10.1038/35023079;
RA   Stover C.K., Pham X.-Q.T., Erwin A.L., Mizoguchi S.D., Warrener P.,
RA   Hickey M.J., Brinkman F.S.L., Hufnagle W.O., Kowalik D.J., Lagrou M.,
RA   Garber R.L., Goltry L., Tolentino E., Westbrock-Wadman S., Yuan Y.,
RA   Brody L.L., Coulter S.N., Folger K.R., Kas A., Larbig K., Lim R.M.,
RA   Smith K.A., Spencer D.H., Wong G.K.-S., Wu Z., Paulsen I.T., Reizer J.,
RA   Saier M.H. Jr., Hancock R.E.W., Lory S., Olson M.V.;
RT   "Complete genome sequence of Pseudomonas aeruginosa PAO1, an opportunistic
RT   pathogen.";
RL   Nature 406:959-964(2000).
CC   -!- FUNCTION: Cytochrome bo(3) ubiquinol terminal oxidase is the component
CC       of the aerobic respiratory chain of E.coli that predominates when cells
CC       are grown at high aeration. Has proton pump activity across the
CC       membrane in addition to electron transfer, pumping 2 protons/electron
CC       (By similarity). {ECO:0000250}.
CC   -!- SUBUNIT: Heterooctamer of two A chains, two B chains, two C chains and
CC       two D chains. {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Cell inner membrane {ECO:0000250}; Multi-pass
CC       membrane protein {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the cytochrome c oxidase bacterial subunit 4
CC       family. {ECO:0000305}.
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DR   EMBL; AE004091; AAG04709.1; -; Genomic_DNA.
DR   PIR; G83480; G83480.
DR   RefSeq; NP_250011.1; NC_002516.2.
DR   RefSeq; WP_003109258.1; NZ_QZGE01000005.1.
DR   AlphaFoldDB; Q9I424; -.
DR   SMR; Q9I424; -.
DR   STRING; 287.DR97_474; -.
DR   PaxDb; Q9I424; -.
DR   EnsemblBacteria; AAG04709; AAG04709; PA1320.
DR   GeneID; 881604; -.
DR   KEGG; pae:PA1320; -.
DR   PATRIC; fig|208964.12.peg.1372; -.
DR   PseudoCAP; PA1320; -.
DR   HOGENOM; CLU_140945_1_1_6; -.
DR   InParanoid; Q9I424; -.
DR   OMA; IVVHMVF; -.
DR   PhylomeDB; Q9I424; -.
DR   BioCyc; PAER208964:G1FZ6-1345-MON; -.
DR   Proteomes; UP000002438; Chromosome.
DR   GO; GO:0009319; C:cytochrome o ubiquinol oxidase complex; IBA:GO_Central.
DR   GO; GO:0005887; C:integral component of plasma membrane; IBA:GO_Central.
DR   GO; GO:0009486; F:cytochrome bo3 ubiquinol oxidase activity; IBA:GO_Central.
DR   GO; GO:0015078; F:proton transmembrane transporter activity; IBA:GO_Central.
DR   GO; GO:0019646; P:aerobic electron transport chain; IBA:GO_Central.
DR   GO; GO:0015990; P:electron transport coupled proton transport; IBA:GO_Central.
DR   InterPro; IPR005171; Cyt_c_oxidase_su4_prok.
DR   InterPro; IPR014210; Cyt_o_ubiqinol_oxidase_su4.
DR   Pfam; PF03626; COX4_pro; 1.
DR   TIGRFAMs; TIGR02847; CyoD; 1.
PE   3: Inferred from homology;
KW   Cell inner membrane; Cell membrane; Electron transport; Membrane;
KW   Oxidoreductase; Reference proteome; Transmembrane; Transmembrane helix;
KW   Transport.
FT   CHAIN           1..111
FT                   /note="Cytochrome bo(3) ubiquinol oxidase subunit 4"
FT                   /id="PRO_0000287758"
FT   TOPO_DOM        1..17
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        18..38
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        39..46
FT                   /note="Periplasmic"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        47..67
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        68..80
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        81..101
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        102..111
FT                   /note="Periplasmic"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   111 AA;  12070 MW;  5E61FEA77E63D1A4 CRC64;
     MSSAAHDNHG AGHGSLGSYA IGFVLSVILT AIPFYMVMDG GFSRHATILT MVVLGLVQVV
     VHLICFLHMN MSSEGRWNVM AFIFTVIVIL LVVGLSLWII FSADMLMMPM P