CYOE_PSEPU
ID CYOE_PSEPU Reviewed; 295 AA.
AC Q9WWR5;
DT 30-MAY-2000, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1999, sequence version 1.
DT 25-MAY-2022, entry version 82.
DE RecName: Full=Protoheme IX farnesyltransferase;
DE EC=2.5.1.141;
DE AltName: Full=Heme O synthase;
GN Name=cyoE;
OS Pseudomonas putida (Arthrobacter siderocapsulatus).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales;
OC Pseudomonadaceae; Pseudomonas.
OX NCBI_TaxID=303;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=IH-2000;
RX PubMed=9868765; DOI=10.1111/j.1574-6968.1998.tb13321.x;
RA Hirayama H., Takami H., Inoue A., Horikoshi K.;
RT "Isolation and characterization of toluene-sensitive mutants from
RT Pseudomonas putida IH-2000.";
RL FEMS Microbiol. Lett. 169:219-225(1998).
CC -!- FUNCTION: Converts protoheme IX and farnesyl diphosphate to heme O.
CC {ECO:0000250}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=(2E,6E)-farnesyl diphosphate + H2O + heme b = diphosphate +
CC Fe(II)-heme o; Xref=Rhea:RHEA:28070, ChEBI:CHEBI:15377,
CC ChEBI:CHEBI:33019, ChEBI:CHEBI:60344, ChEBI:CHEBI:60530,
CC ChEBI:CHEBI:175763; EC=2.5.1.141;
CC -!- COFACTOR:
CC Name=Mg(2+); Xref=ChEBI:CHEBI:18420; Evidence={ECO:0000250};
CC Name=Ca(2+); Xref=ChEBI:CHEBI:29108; Evidence={ECO:0000250};
CC Note=Divalent metal cations. Mg(2+) or Ca(2+). {ECO:0000250};
CC -!- SUBCELLULAR LOCATION: Cell inner membrane {ECO:0000250}; Multi-pass
CC membrane protein {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the UbiA prenyltransferase family.
CC {ECO:0000305}.
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DR EMBL; AB016787; BAA76360.1; -; Genomic_DNA.
DR RefSeq; WP_025337747.1; NZ_NFSB01000087.1.
DR AlphaFoldDB; Q9WWR5; -.
DR SMR; Q9WWR5; -.
DR STRING; 1240350.AMZE01000009_gene4208; -.
DR eggNOG; COG0109; Bacteria.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0008495; F:protoheme IX farnesyltransferase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0048034; P:heme O biosynthetic process; IEA:UniProtKB-UniRule.
DR CDD; cd13957; PT_UbiA_Cox10; 1.
DR Gene3D; 1.10.357.140; -; 1.
DR HAMAP; MF_00154; CyoE_CtaB; 1.
DR InterPro; IPR006369; Protohaem_IX_farnesylTrfase.
DR InterPro; IPR000537; UbiA_prenyltransferase.
DR InterPro; IPR030470; UbiA_prenylTrfase_CS.
DR InterPro; IPR044878; UbiA_sf.
DR PANTHER; PTHR43448; PTHR43448; 1.
DR Pfam; PF01040; UbiA; 1.
DR TIGRFAMs; TIGR01473; cyoE_ctaB; 1.
DR PROSITE; PS00943; UBIA; 1.
PE 3: Inferred from homology;
KW Cell inner membrane; Cell membrane; Heme biosynthesis; Membrane;
KW Transferase; Transmembrane; Transmembrane helix.
FT CHAIN 1..295
FT /note="Protoheme IX farnesyltransferase"
FT /id="PRO_0000162901"
FT TOPO_DOM 1..9
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 10..28
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 29..37
FT /note="Periplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 38..56
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 57..78
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 79..97
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 98..107
FT /note="Periplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 108..126
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 127..197
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 198..216
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 217..228
FT /note="Periplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 229..247
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 248..268
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 269..287
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 288..295
FT /note="Periplasmic"
FT /evidence="ECO:0000255"
SQ SEQUENCE 295 AA; 31764 MW; AA5D1BDD44664D0C CRC64;
MSVKHFIQIT KPGIIFGNVL SVAGGFFLAS KGHVDFALFL AVVIGTSLVV ASGCVFNNCI
DRDIDHKMER TKNRVMVQGG MSLPLALIYA TLLGVAGFSL LYVQANPLSA FCALIGFIVY
VGFYSLWLKR KSVHGTLVGS LSGAMPPVIG YCAVSNSFDL AAVTLLVMFS LWQMPHSFAI
AIFRFKDYSA ANIPVLPVAR GILAAKKQIV LYVLAFVLAT LMLTLGGYAG LGYLAVAAAM
GLYWLYMAWG GYKAEDDSKW ARKVFGFSIL TVTALSVMMG VDSQTAADVL MTYAR