CYP31_DOTSN
ID CYP31_DOTSN Reviewed; 317 AA.
AC M2XJ44;
DT 18-SEP-2019, integrated into UniProtKB/Swiss-Prot.
DT 01-MAY-2013, sequence version 1.
DT 03-AUG-2022, entry version 35.
DE RecName: Full=Hps1-dma1 cluster cytochrome P450 monooxygenase cyp3.1 {ECO:0000303|PubMed:23209441};
GN Name=cyp3.1; ORFNames=DOTSEDRAFT_75219;
OS Dothistroma septosporum (strain NZE10 / CBS 128990) (Red band needle blight
OS fungus) (Mycosphaerella pini).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Dothideomycetes;
OC Dothideomycetidae; Mycosphaerellales; Mycosphaerellaceae; Dothistroma.
OX NCBI_TaxID=675120;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=NZE10 / CBS 128990;
RX PubMed=23209441; DOI=10.1371/journal.pgen.1003088;
RA de Wit P.J.G.M., van der Burgt A., Oekmen B., Stergiopoulos I.,
RA Abd-Elsalam K.A., Aerts A.L., Bahkali A.H., Beenen H.G., Chettri P.,
RA Cox M.P., Datema E., de Vries R.P., Dhillon B., Ganley A.R.,
RA Griffiths S.A., Guo Y., Hamelin R.C., Henrissat B., Kabir M.S.,
RA Jashni M.K., Kema G., Klaubauf S., Lapidus A., Levasseur A., Lindquist E.,
RA Mehrabi R., Ohm R.A., Owen T.J., Salamov A., Schwelm A., Schijlen E.,
RA Sun H., van den Burg H.A., van Ham R.C.H.J., Zhang S., Goodwin S.B.,
RA Grigoriev I.V., Collemare J., Bradshaw R.E.;
RT "The genomes of the fungal plant pathogens Cladosporium fulvum and
RT Dothistroma septosporum reveal adaptation to different hosts and lifestyles
RT but also signatures of common ancestry.";
RL PLoS Genet. 8:E1003088-E1003088(2012).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=NZE10 / CBS 128990;
RX PubMed=23236275; DOI=10.1371/journal.ppat.1003037;
RA Ohm R.A., Feau N., Henrissat B., Schoch C.L., Horwitz B.A., Barry K.W.,
RA Condon B.J., Copeland A.C., Dhillon B., Glaser F., Hesse C.N., Kosti I.,
RA LaButti K., Lindquist E.A., Lucas S., Salamov A.A., Bradshaw R.E.,
RA Ciuffetti L., Hamelin R.C., Kema G.H.J., Lawrence C., Scott J.A.,
RA Spatafora J.W., Turgeon B.G., de Wit P.J.G.M., Zhong S., Goodwin S.B.,
RA Grigoriev I.V.;
RT "Diverse lifestyles and strategies of plant pathogenesis encoded in the
RT genomes of eighteen Dothideomycetes fungi.";
RL PLoS Pathog. 8:E1003037-E1003037(2012).
RN [3]
RP FUNCTION, AND PATHWAY.
RX PubMed=31053329; DOI=10.1016/j.funbio.2019.02.006;
RA Ozturk I.K., Dupont P.Y., Chettri P., McDougal R., Boehl O.J., Cox R.J.,
RA Bradshaw R.E.;
RT "Evolutionary relics dominate the small number of secondary metabolism
RT genes in the hemibiotrophic fungus Dothistroma septosporum.";
RL Fungal Biol. 123:397-407(2019).
CC -!- FUNCTION: Cytochrome P450 monooxygenase; part of the hps1-dma1 gene
CC cluster that probably mediates the biosynthesis a derivative of
CC cyclopiazonic acid (CPA) (Probable). The hybrid polyketide synthase-
CC nonribosomal peptide synthetase (PKS-NRPS) nps1 might incorporates
CC acetyl-CoA, malonyl-CoA, and tryptophan (Trp) and utilizes a C-terminal
CC redox-incompetent reductase domain to make and release the tryptophan
CC tetramic acid, cyclo-acetoacetyl-L-tryptophan (c-AATrp), as the first
CC intermediate in the pathway (By similarity). In addition, the cluster
CC also includes the tryptophan dimethylallyltransferase dma1, the FAD-
CC dependent oxidoreductase toxD, the cytochrome P450 monooxygenase cyp3.1
CC and the methyltransferase DOTSEDRAFT_139328; the latter 2 being not
CC present in all CPA-producing fungi but involved in additional
CC modifications that occur in biosynthesis the of a range of CPA and CPA-
CC like products (Probable). Further studies are required to clarify
CC whether the CPA-like hps1-dma1 cluster is functional or a non-
CC functional relic reflecting evolution of D.septosporum (Probable).
CC {ECO:0000250|UniProtKB:B6F209, ECO:0000305|PubMed:31053329}.
CC -!- COFACTOR:
CC Name=heme; Xref=ChEBI:CHEBI:30413;
CC Evidence={ECO:0000250|UniProtKB:P04798};
CC -!- PATHWAY: Secondary metabolite biosynthesis.
CC {ECO:0000305|PubMed:31053329}.
CC -!- SIMILARITY: Belongs to the cytochrome P450 family. {ECO:0000305}.
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DR EMBL; KB446545; EME39477.1; -; Genomic_DNA.
DR AlphaFoldDB; M2XJ44; -.
DR SMR; M2XJ44; -.
DR STRING; 675120.M2XJ44; -.
DR EnsemblFungi; EME39477; EME39477; DOTSEDRAFT_75219.
DR eggNOG; KOG0157; Eukaryota.
DR HOGENOM; CLU_083058_0_0_1; -.
DR OMA; DVFMLTN; -.
DR OrthoDB; 702827at2759; -.
DR Proteomes; UP000016933; Unassembled WGS sequence.
DR GO; GO:0020037; F:heme binding; IEA:InterPro.
DR GO; GO:0005506; F:iron ion binding; IEA:InterPro.
DR GO; GO:0004497; F:monooxygenase activity; IEA:InterPro.
DR GO; GO:0016705; F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen; IEA:InterPro.
DR Gene3D; 1.10.630.10; -; 1.
DR InterPro; IPR001128; Cyt_P450.
DR InterPro; IPR002403; Cyt_P450_E_grp-IV.
DR InterPro; IPR036396; Cyt_P450_sf.
DR Pfam; PF00067; p450; 2.
DR PRINTS; PR00465; EP450IV.
DR PRINTS; PR00385; P450.
DR SUPFAM; SSF48264; SSF48264; 1.
PE 3: Inferred from homology;
KW Iron; Metal-binding; Reference proteome.
FT CHAIN 1..317
FT /note="Hps1-dma1 cluster cytochrome P450 monooxygenase
FT cyp3.1"
FT /id="PRO_0000447730"
FT REGION 183..205
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT BINDING 260
FT /ligand="heme"
FT /ligand_id="ChEBI:CHEBI:30413"
FT /ligand_part="Fe"
FT /ligand_part_id="ChEBI:CHEBI:18248"
FT /note="axial binding residue"
FT /evidence="ECO:0000250|UniProtKB:P04798"
SQ SEQUENCE 317 AA; 35346 MW; B69F5F3272E94871 CRC64;
MGTYYRRKDQ FLLAKIHEAE ARMTGEDSSG SSARIRRRSA LDHFVRQEMD AAARAGRAPD
FLHGPIRDEL LGYIIAGHDS STSALSWALK YLTTDARVQH ALRQHLHAVH AASWREGRTP
SVAEITSIRA PYLDAVIQEV FRCAYTTPFT LREATRDTQI MGHFVPKGTT VWFSTSGPSF
TRPAIETDRK TSSHSRSPTA LADKQRVPAW SPQDVALFRP ERWLRPDPGP SDPPDFAYSH
VDFNGNAGPM MAFGSGPRGC CGKRLVYLSM RILVTLVLWD FDLLPCPTRL SSDESNHGTA
SHPKQCYLRL ATTTHGP
