CYPLD_PSYLE
ID CYPLD_PSYLE Reviewed; 29 AA.
AC C0HL30;
DT 25-OCT-2017, integrated into UniProtKB/Swiss-Prot.
DT 25-OCT-2017, sequence version 1.
DT 25-MAY-2022, entry version 6.
DE RecName: Full=Cyclotide psyleio D {ECO:0000303|PubMed:28006906};
OS Psychotria leiocarpa.
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC asterids; lamiids; Gentianales; Rubiaceae; Rubioideae; Psychotrieae;
OC Psychotria.
OX NCBI_TaxID=2022332;
RN [1] {ECO:0000305}
RP PROTEIN SEQUENCE, MASS SPECTROMETRY, IDENTIFICATION BY MASS SPECTROMETRY,
RP AND CYCLIZATION.
RC TISSUE=Leaf {ECO:0000303|PubMed:28006906};
RX PubMed=28006906; DOI=10.1021/acs.jnatprod.6b00492;
RA Matsuura H.N., Poth A.G., Yendo A.C., Fett-Neto A.G., Craik D.J.;
RT "Isolation and Characterization of Cyclotides from Brazilian Psychotria:
RT Significance in Plant Defense and Co-occurrence with Antioxidant
RT Alkaloids.";
RL J. Nat. Prod. 79:3006-3013(2016).
CC -!- FUNCTION: Probably participates in a plant defense mechanism.
CC {ECO:0000255|PROSITE-ProRule:PRU00395}.
CC -!- DOMAIN: The presence of a 'disulfide through disulfide knot'
CC structurally defines this protein as a knottin. {ECO:0000305}.
CC -!- PTM: This is a cyclic peptide. {ECO:0000255|PROSITE-ProRule:PRU00395,
CC ECO:0000269|PubMed:28006906}.
CC -!- MASS SPECTROMETRY: Mass=3291.17; Method=MALDI;
CC Evidence={ECO:0000269|PubMed:28006906};
CC -!- SIMILARITY: Belongs to the cyclotide family. Moebius subfamily.
CC {ECO:0000255|PROSITE-ProRule:PRU00395}.
CC -!- CAUTION: This peptide is cyclic. The start position was chosen by
CC similarity to Oak1 (kalata B1) for which the DNA sequence is known.
CC {ECO:0000255|PROSITE-ProRule:PRU00395}.
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DR AlphaFoldDB; C0HL30; -.
DR SMR; C0HL30; -.
DR GO; GO:0006952; P:defense response; IEA:UniProtKB-KW.
DR InterPro; IPR005535; Cyclotide.
DR InterPro; IPR012324; Cyclotide_moebius_CS.
DR InterPro; IPR036146; Cyclotide_sf.
DR Pfam; PF03784; Cyclotide; 1.
DR PIRSF; PIRSF037891; Cycloviolacin; 1.
DR SUPFAM; SSF57038; SSF57038; 1.
DR PROSITE; PS51052; CYCLOTIDE; 1.
DR PROSITE; PS60009; CYCLOTIDE_MOEBIUS; 1.
PE 1: Evidence at protein level;
KW Direct protein sequencing; Disulfide bond; Knottin; Plant defense.
FT PEPTIDE 1..29
FT /note="Cyclotide psyleio D"
FT /evidence="ECO:0000269|PubMed:28006906"
FT /id="PRO_0000441795"
FT DISULFID 5..19
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00395"
FT DISULFID 9..21
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00395"
FT DISULFID 14..26
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00395"
FT CROSSLNK 1..29
FT /note="Cyclopeptide (Gly-Asp)"
FT /evidence="ECO:0000305|PubMed:28006906"
FT UNSURE 2
FT /note="L or I"
FT /evidence="ECO:0000303|PubMed:28006906"
SQ SEQUENCE 29 AA; 2951 MW; 27C8B0F38887FA4C CRC64;
GLPVCGESCF GGTCNTPGCS CTWPVCTRD