CYPR1_CYNCA
ID CYPR1_CYNCA Reviewed; 473 AA.
AC P40782;
DT 01-FEB-1995, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1995, sequence version 2.
DT 03-AUG-2022, entry version 108.
DE RecName: Full=Cyprosin;
DE EC=3.4.23.-;
DE Flags: Precursor; Fragment;
GN Name=CYPRO1;
OS Cynara cardunculus (Cardoon).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC asterids; campanulids; Asterales; Asteraceae; Carduoideae; Cardueae;
OC Carduinae; Cynara.
OX NCBI_TaxID=4265;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], AND PROTEIN SEQUENCE OF 178-186.
RC TISSUE=Flower bud;
RX PubMed=8193298; DOI=10.1007/bf00029855;
RA Cordeiro M.C., Xue Z.-T., Pietrzak M., Pais M.S., Brodelius P.E.;
RT "Isolation and characterization of a cDNA from flowers of Cynara
RT cardunculus encoding cyprosin (an aspartic proteinase) and its use to study
RT the organ-specific expression of cyprosin.";
RL Plant Mol. Biol. 24:733-741(1994).
CC -!- TISSUE SPECIFICITY: Mostly present in the violet parts of styles and
CC corollas of mature flowers.
CC -!- DEVELOPMENTAL STAGE: Expressed in early stages of floral development
CC and switched off at maturation of the flower.
CC -!- SIMILARITY: Belongs to the peptidase A1 family. {ECO:0000305}.
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DR EMBL; X69193; CAA48939.1; ALT_SEQ; mRNA.
DR PIR; S47096; S47096.
DR PIR; T12049; T12049.
DR AlphaFoldDB; P40782; -.
DR SMR; P40782; -.
DR MEROPS; A01.A02; -.
DR PRIDE; P40782; -.
DR GO; GO:0004190; F:aspartic-type endopeptidase activity; IEA:UniProtKB-KW.
DR GO; GO:0006629; P:lipid metabolic process; IEA:InterPro.
DR GO; GO:0006508; P:proteolysis; IEA:UniProtKB-KW.
DR CDD; cd06098; phytepsin; 1.
DR Gene3D; 2.40.70.10; -; 2.
DR InterPro; IPR001461; Aspartic_peptidase_A1.
DR InterPro; IPR001969; Aspartic_peptidase_AS.
DR InterPro; IPR033121; PEPTIDASE_A1.
DR InterPro; IPR021109; Peptidase_aspartic_dom_sf.
DR InterPro; IPR033869; Phytepsin.
DR InterPro; IPR007856; SapB_1.
DR InterPro; IPR008138; SapB_2.
DR InterPro; IPR011001; Saposin-like.
DR InterPro; IPR008139; SaposinB_dom.
DR PANTHER; PTHR47966; PTHR47966; 1.
DR Pfam; PF00026; Asp; 1.
DR Pfam; PF05184; SapB_1; 1.
DR Pfam; PF03489; SapB_2; 1.
DR PRINTS; PR00792; PEPSIN.
DR SMART; SM00741; SapB; 1.
DR SUPFAM; SSF47862; SSF47862; 1.
DR SUPFAM; SSF50630; SSF50630; 1.
DR PROSITE; PS00141; ASP_PROTEASE; 2.
DR PROSITE; PS51767; PEPTIDASE_A1; 1.
DR PROSITE; PS50015; SAP_B; 2.
PE 1: Evidence at protein level;
KW Aspartyl protease; Direct protein sequencing; Disulfide bond; Glycoprotein;
KW Hydrolase; Protease; Zymogen.
FT PROPEP <1..33
FT /note="Activation peptide"
FT /evidence="ECO:0000255"
FT /id="PRO_0000025899"
FT CHAIN 34..473
FT /note="Cyprosin"
FT /id="PRO_0000025900"
FT DOMAIN 51..470
FT /note="Peptidase A1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01103"
FT DOMAIN 281..384
FT /note="Saposin B-type"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00415"
FT ACT_SITE 69
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU10094"
FT ACT_SITE 256
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU10094"
FT CARBOHYD 364
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00415"
FT DISULFID 82..88
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00415"
FT DISULFID 247..251
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00415"
FT DISULFID 286..378
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00415"
FT DISULFID 311..350
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00415"
FT DISULFID 317..347
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00415"
FT DISULFID 392..429
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00415"
FT NON_TER 1
SQ SEQUENCE 473 AA; 51564 MW; 65F3232EBD06CB56 CRC64;
LKKRKVNILN HPGEHAGSND ANARRKYGVR GNFRDSDGEL IALKNYMDAQ YFGEIGIGTP
PQKFTVIFDT GSSNLWVPSS KCYFSVACLF HSKYRSTDST TYKKNGKSAA IQYGTGSISG
FFSQDSVKLG DLLVKEQDFI EATKEPGITF LAAKFDGILG LGFQEISVGD AVPVWYTMLN
QGLVQEPVFS FWLNRNADEQ EGGELVFGGV DPNHFKGEHT YVPVTQKGYW QFEMGDVLIG
DKTTGFCASG CAAIADSGTS LLAGTTTIVT QINQAIGAAG VMSQQCKSLV DQYGKSMIEM
LLSEEQPEKI CSQMKLCSFD GSHDTSMIIE SVVDKSKGKS SGLPMRCVPC ARWVVWMQNQ
IRQNETEENI INYVDKLCER LPSPMGESAV DCSSLSSMPN IAFTVGGKTF NLSPEQYVLK
VGEGATAQCI SGFTAMDVAP PHGPLWILGD VFMGQYHTVF DYGNLRVGFA EAA