ID   CYQ32_CATRO             Reviewed;         499 AA.
AC   W8JMU7;
DT   10-APR-2019, integrated into UniProtKB/Swiss-Prot.
DT   14-MAY-2014, sequence version 1.
DT   03-AUG-2022, entry version 21.
DE   RecName: Full=Cytochrome P450 81Q32 {ECO:0000303|PubMed:24710322};
DE            Short=CrCYP81Q32 {ECO:0000303|PubMed:24710322};
DE            EC=1.14.-.- {ECO:0000305};
GN   Name=CYP81Q32 {ECO:0000303|PubMed:24710322};
OS   Catharanthus roseus (Madagascar periwinkle) (Vinca rosea).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   asterids; lamiids; Gentianales; Apocynaceae; Rauvolfioideae; Vinceae;
OC   Catharanthinae; Catharanthus.
OX   NCBI_TaxID=4058;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA], AND TISSUE SPECIFICITY.
RC   STRAIN=cv. Little Bright Eyes;
RX   PubMed=24710322; DOI=10.1038/ncomms4606;
RA   Miettinen K., Dong L., Navrot N., Schneider T., Burlat V., Pollier J.,
RA   Woittiez L., van der Krol S., Lugan R., Ilc T., Verpoorte R.,
RA   Oksman-Caldentey K.M., Martinoia E., Bouwmeester H., Goossens A.,
RA   Memelink J., Werck-Reichhart D.;
RT   "The seco-iridoid pathway from Catharanthus roseus.";
RL   Nat. Commun. 5:3606-3606(2014).
CC   -!- SUBCELLULAR LOCATION: Membrane {ECO:0000255}; Single-pass membrane
CC       protein {ECO:0000255}.
CC   -!- TISSUE SPECIFICITY: Expressed in leaf epidermis and in the leaf
CC       internal phloem-associated parenchyma (IPAP) inside the mesophyll.
CC       {ECO:0000269|PubMed:24710322}.
CC   -!- SIMILARITY: Belongs to the cytochrome P450 family. {ECO:0000305}.
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DR   EMBL; KF302070; AHK60837.1; -; mRNA.
DR   AlphaFoldDB; W8JMU7; -.
DR   SMR; W8JMU7; -.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0020037; F:heme binding; IEA:InterPro.
DR   GO; GO:0005506; F:iron ion binding; IEA:InterPro.
DR   GO; GO:0004497; F:monooxygenase activity; IEA:UniProtKB-KW.
DR   GO; GO:0016705; F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen; IEA:InterPro.
DR   Gene3D; 1.10.630.10; -; 1.
DR   InterPro; IPR001128; Cyt_P450.
DR   InterPro; IPR017972; Cyt_P450_CS.
DR   InterPro; IPR002401; Cyt_P450_E_grp-I.
DR   InterPro; IPR036396; Cyt_P450_sf.
DR   Pfam; PF00067; p450; 1.
DR   PRINTS; PR00463; EP450I.
DR   PRINTS; PR00385; P450.
DR   SUPFAM; SSF48264; SSF48264; 1.
DR   PROSITE; PS00086; CYTOCHROME_P450; 1.
PE   2: Evidence at transcript level;
KW   Glycoprotein; Heme; Iron; Membrane; Metal-binding; Monooxygenase;
KW   Oxidoreductase; Transmembrane; Transmembrane helix.
FT   CHAIN           1..499
FT                   /note="Cytochrome P450 81Q32"
FT                   /id="PRO_0000446416"
FT   TRANSMEM        5..25
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   BINDING         434
FT                   /ligand="heme"
FT                   /ligand_id="ChEBI:CHEBI:30413"
FT                   /ligand_part="Fe"
FT                   /ligand_part_id="ChEBI:CHEBI:18248"
FT                   /note="axial binding residue"
FT                   /evidence="ECO:0000250|UniProtKB:P04798"
FT   CARBOHYD        112
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
FT   CARBOHYD        183
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
FT   CARBOHYD        266
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
SQ   SEQUENCE   499 AA;  56111 MW;  3C248F0B64AF908D CRC64;
     MESSTLLYTF LAVVLLSISL KLFPVSRRRR NLPPSPGLAL PVIGHLHLIG KLLHRSLYDL
     SKKYGSVFSL QLGNRLVLVV SSPAAAEECF TKNDIVFANR PLFILGKYIG YNYTTMVGSP
     YGEHWRNLRR LAAVEIFSAG SLNRFLSIRE DEVKQLLLSL YQSSGQDFGK VEMKSKLSEL
     SFNVTMRMVA GKRYFGQDVD SDEAKLFRAL IGEVFEHAGA SNPGDFVPFL RWIDFKNYEK
     KVSKISQEMD AFLQRLIHES RINKNNVTMI DHLLSLQESQ PEYYTDQIIK GIIMVLLLAG
     TDTSAVTVEW AMSLLLNHPE TLEKARTEIE TQVGSNRLIE EQDLPKLTYL HNIISETFRL
     CPAAPMLVPH ESSDDCKVQG YDVPKGTILL VNAWAIHRDP EFWDEPTLFK PERHGGVELE
     PSKLMPFGMG RRSCPGSGLA QRVVGLTLGA LIQCFEWKRI GEAKIDMAEG SGLTMPKAQP
     LEALCKPRNI LHKVVSETS