CYREN_CRIGR
ID CYREN_CRIGR Reviewed; 154 AA.
AC Q09HN1;
DT 26-FEB-2008, integrated into UniProtKB/Swiss-Prot.
DT 17-OCT-2006, sequence version 1.
DT 25-MAY-2022, entry version 37.
DE RecName: Full=Cell cycle regulator of non-homologous end joining {ECO:0000250|UniProtKB:Q9BWK5};
DE Short=Cell cycle regulator of NHEJ {ECO:0000250|UniProtKB:Q9BWK5};
DE AltName: Full=Modulator of retrovirus infection {ECO:0000303|PubMed:17043244};
GN Name=CYREN {ECO:0000250|UniProtKB:Q9BWK5};
GN Synonyms=MRI {ECO:0000303|PubMed:17043244};
OS Cricetulus griseus (Chinese hamster) (Cricetulus barabensis griseus).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea;
OC Cricetidae; Cricetinae; Cricetulus.
OX NCBI_TaxID=10029;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, AND SUBCELLULAR LOCATION.
RC TISSUE=Lung;
RX PubMed=17043244; DOI=10.1073/pnas.0602674103;
RA Agarwal S., Harada J., Schreifels J., Lech P., Nikolai B., Yamaguchi T.,
RA Chanda S.K., Somia N.V.;
RT "Isolation, characterization, and genetic complementation of a cellular
RT mutant resistant to retroviral infection.";
RL Proc. Natl. Acad. Sci. U.S.A. 103:15933-15938(2006).
CC -!- FUNCTION: Cell-cycle-specific regulator of classical non-homologous end
CC joining (NHEJ) of DNA double-strand break (DSB) repair, which can act
CC both as an activator or inhibitor of NHEJ, depending on the cell cycle
CC phase (By similarity). Acts as a regulator of DNA repair pathway choice
CC by specifically inhibiting classical NHEJ during the S and G2 phases,
CC thereby promoting error-free repair by homologous recombination during
CC cell cycle phases when sister chromatids are present. Preferentially
CC protects single-stranded overhangs at break sites by inhibiting
CC classical NHEJ, thereby creating a local environment that favors
CC homologous recombination. Acts via interaction with XRCC5/Ku80 and
CC XRCC6/Ku70 (By similarity). In contrast, acts as an activator of NHEJ
CC during G1 phase of the cell cycle: promotes classical NHEJ in G1 phase
CC cells via multivalent interactions that increase the affinity of DNA
CC damage response proteins for DSB-associated chromatin. Also involved in
CC immunoglobulin V(D)J recombination (By similarity). May act as a
CC regulator of proteasome (PubMed:17043244). In case of infection by a
CC retrovirus, may regulate the proteasome during the uncoating phase of
CC retrovirus (PubMed:17043244). {ECO:0000250|UniProtKB:Q8BHZ5,
CC ECO:0000250|UniProtKB:Q9BWK5, ECO:0000269|PubMed:17043244}.
CC -!- SUBUNIT: Interacts (via KBM motif) with XRCC5/Ku80 and XRCC6/Ku70
CC heterodimer. Interacts (via XLF motif) with TRIM28/KAP1, ATM, MRE11,
CC NBN and RAD50. {ECO:0000250|UniProtKB:Q8BHZ5}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000269|PubMed:17043244}. Nucleus
CC {ECO:0000250|UniProtKB:Q9BWK5}. Chromosome
CC {ECO:0000250|UniProtKB:Q8BHZ5}. Note=Nuclear localization may depend
CC upon interaction with XRCC5/Ku80 and XRCC6/Ku70 heterodimer (By
CC similarity). Localizes to DNA damage sites (By similarity).
CC {ECO:0000250|UniProtKB:Q8BHZ5, ECO:0000250|UniProtKB:Q9BWK5}.
CC -!- DOMAIN: The KBM (Ku-binding motif) mediates interaction with XRCC5/Ku80
CC and XRCC6/Ku70 and recruitment to DNA damage sites.
CC {ECO:0000250|UniProtKB:Q8BHZ5}.
CC -!- DOMAIN: The XLM (XLF-like motif) mediates interaction with DNA damage
CC response proteins TRIM28/KAP1, ATM and members of the MRN complex
CC (MRE11, NBN and RAD50). {ECO:0000250|UniProtKB:Q8BHZ5}.
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DR EMBL; DQ899952; ABI64109.1; -; mRNA.
DR RefSeq; NP_001233721.1; NM_001246792.1.
DR AlphaFoldDB; Q09HN1; -.
DR STRING; 10029.NP_001233721.1; -.
DR GeneID; 100689364; -.
DR KEGG; cge:100689364; -.
DR CTD; 78996; -.
DR eggNOG; ENOG502SEX2; Eukaryota.
DR OrthoDB; 1349796at2759; -.
DR GO; GO:0005737; C:cytoplasm; ISS:UniProtKB.
DR GO; GO:0005634; C:nucleus; ISS:UniProtKB.
DR GO; GO:0035861; C:site of double-strand break; ISS:UniProtKB.
DR GO; GO:0006303; P:double-strand break repair via nonhomologous end joining; ISS:UniProtKB.
DR GO; GO:0033152; P:immunoglobulin V(D)J recombination; ISS:UniProtKB.
DR GO; GO:2001033; P:negative regulation of double-strand break repair via nonhomologous end joining; ISS:UniProtKB.
DR InterPro; IPR028278; MRI.
DR PANTHER; PTHR14566; PTHR14566; 1.
DR Pfam; PF15325; MRI; 1.
PE 2: Evidence at transcript level;
KW Acetylation; Chromosome; Cytoplasm; DNA damage; DNA repair; Nucleus.
FT CHAIN 1..154
FT /note="Cell cycle regulator of non-homologous end joining"
FT /id="PRO_0000320947"
FT REGION 77..144
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOTIF 1..21
FT /note="KBM"
FT /evidence="ECO:0000250|UniProtKB:Q9BWK5"
FT MOTIF 144..154
FT /note="XLM"
FT /evidence="ECO:0000250|UniProtKB:Q9BWK5"
FT COMPBIAS 97..111
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 1
FT /note="N-acetylmethionine"
FT /evidence="ECO:0000250|UniProtKB:Q9BWK5"
SQ SEQUENCE 154 AA; 16850 MW; 9C65BDFB9C27BF5D CRC64;
METLKSENKK RVLPSWMTAP VDEKRELSVK TPKRKKIAAG QVGLATRAPV MKTVYCMNEA
EMVDVALGIL IEGRKQEEPT LVAPDKPQPS PPYSASPHTS SPGSSSEKED SGNRWPALGL
SPSHGPEAAD SPCSRSPEEE EEDALKYVRE IFFS
