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CYS3_NEUCR
ID   CYS3_NEUCR              Reviewed;         236 AA.
AC   P22697; Q7RU61;
DT   01-AUG-1991, integrated into UniProtKB/Swiss-Prot.
DT   01-AUG-1991, sequence version 1.
DT   25-MAY-2022, entry version 121.
DE   RecName: Full=Regulatory protein cys-3;
GN   Name=cys-3; ORFNames=B2A19.70, NCU21522;
OS   Neurospora crassa (strain ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 /
OS   FGSC 987).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Sordariomycetes;
OC   Sordariomycetidae; Sordariales; Sordariaceae; Neurospora.
OX   NCBI_TaxID=367110;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=2524646; DOI=10.1128/mcb.9.3.1120-1127.1989;
RA   Fu Y.-H., Paietta J.V., Mannix D.G., Marzluf G.A.;
RT   "cys-3, the positive-acting sulfur regulatory gene of Neurospora crassa,
RT   encodes a protein with a putative leucine zipper DNA-binding element.";
RL   Mol. Cell. Biol. 9:1120-1127(1989).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987;
RX   PubMed=12655011; DOI=10.1093/nar/gkg293;
RA   Mannhaupt G., Montrone C., Haase D., Mewes H.-W., Aign V., Hoheisel J.D.,
RA   Fartmann B., Nyakatura G., Kempken F., Maier J., Schulte U.;
RT   "What's in the genome of a filamentous fungus? Analysis of the Neurospora
RT   genome sequence.";
RL   Nucleic Acids Res. 31:1944-1954(2003).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987;
RX   PubMed=12712197; DOI=10.1038/nature01554;
RA   Galagan J.E., Calvo S.E., Borkovich K.A., Selker E.U., Read N.D.,
RA   Jaffe D.B., FitzHugh W., Ma L.-J., Smirnov S., Purcell S., Rehman B.,
RA   Elkins T., Engels R., Wang S., Nielsen C.B., Butler J., Endrizzi M.,
RA   Qui D., Ianakiev P., Bell-Pedersen D., Nelson M.A., Werner-Washburne M.,
RA   Selitrennikoff C.P., Kinsey J.A., Braun E.L., Zelter A., Schulte U.,
RA   Kothe G.O., Jedd G., Mewes H.-W., Staben C., Marcotte E., Greenberg D.,
RA   Roy A., Foley K., Naylor J., Stange-Thomann N., Barrett R., Gnerre S.,
RA   Kamal M., Kamvysselis M., Mauceli E.W., Bielke C., Rudd S., Frishman D.,
RA   Krystofova S., Rasmussen C., Metzenberg R.L., Perkins D.D., Kroken S.,
RA   Cogoni C., Macino G., Catcheside D.E.A., Li W., Pratt R.J., Osmani S.A.,
RA   DeSouza C.P.C., Glass N.L., Orbach M.J., Berglund J.A., Voelker R.,
RA   Yarden O., Plamann M., Seiler S., Dunlap J.C., Radford A., Aramayo R.,
RA   Natvig D.O., Alex L.A., Mannhaupt G., Ebbole D.J., Freitag M., Paulsen I.,
RA   Sachs M.S., Lander E.S., Nusbaum C., Birren B.W.;
RT   "The genome sequence of the filamentous fungus Neurospora crassa.";
RL   Nature 422:859-868(2003).
RN   [4]
RP   CHARACTERIZATION.
RX   PubMed=2142156; DOI=10.1016/s0021-9258(19)38491-1;
RA   Fu Y.-H., Marzluf G.A.;
RT   "cys-3, the positive-acting sulfur regulatory gene of Neurospora crassa,
RT   encodes a sequence-specific DNA-binding protein.";
RL   J. Biol. Chem. 265:11942-11947(1990).
RN   [5]
RP   MUTAGENESIS.
RX   PubMed=1831537; DOI=10.1128/mcb.11.9.4356-4362.1991;
RA   Kanaan M.N., Marzluf G.A.;
RT   "Mutational analysis of the DNA-binding domain of the CYS3 regulatory
RT   protein of Neurospora crassa.";
RL   Mol. Cell. Biol. 11:4356-4362(1991).
RN   [6]
RP   MUTAGENESIS.
RX   PubMed=1532511; DOI=10.1021/bi00127a022;
RA   Kanaan M.N., Fu Y.-H., Marzluf G.A.;
RT   "The DNA-binding domain of the Cys-3 regulatory protein of Neurospora
RT   crassa is bipartite.";
RL   Biochemistry 31:3197-3203(1992).
CC   -!- FUNCTION: Turns on the expression of structural genes which encode
CC       sulfur-catabolic enzymes. Binds to sequence elements upstream of these
CC       genes.
CC   -!- SUBUNIT: Binds DNA as a dimer.
CC   -!- SUBCELLULAR LOCATION: Nucleus.
CC   -!- INDUCTION: By sulfur deprivation.
CC   -!- SIMILARITY: Belongs to the bZIP family. GCN4 subfamily. {ECO:0000305}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=EAA27579.2; Type=Erroneous gene model prediction; Note=The predicted gene NCU03536 has been split into 2 genes: NCU21521 and NCU21522.; Evidence={ECO:0000305};
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DR   EMBL; M26008; AAA33585.1; -; Genomic_DNA.
DR   EMBL; AL390092; CAB98237.1; -; Genomic_DNA.
DR   EMBL; CM002237; EAA27579.2; ALT_SEQ; Genomic_DNA.
DR   PIR; A30225; A30225.
DR   PIR; T51073; T51073.
DR   AlphaFoldDB; P22697; -.
DR   SMR; P22697; -.
DR   STRING; 5141.EFNCRP00000002554; -.
DR   EnsemblFungi; EAA27579; EAA27579; NCU03536.
DR   HOGENOM; CLU_056562_0_0_1; -.
DR   InParanoid; P22697; -.
DR   Proteomes; UP000001805; Chromosome 6, Linkage Group II.
DR   GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0003700; F:DNA-binding transcription factor activity; IEA:InterPro.
DR   InterPro; IPR004827; bZIP.
DR   InterPro; IPR046347; bZIP_sf.
DR   Pfam; PF07716; bZIP_2; 1.
DR   SMART; SM00338; BRLZ; 1.
DR   SUPFAM; SSF57959; SSF57959; 1.
DR   PROSITE; PS50217; BZIP; 1.
DR   PROSITE; PS00036; BZIP_BASIC; 1.
PE   1: Evidence at protein level;
KW   Activator; DNA-binding; Nucleus; Reference proteome; Stress response;
KW   Transcription; Transcription regulation.
FT   CHAIN           1..236
FT                   /note="Regulatory protein cys-3"
FT                   /id="PRO_0000076489"
FT   DOMAIN          99..162
FT                   /note="bZIP"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00978"
FT   REGION          26..89
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          105..137
FT                   /note="Basic motif"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00978"
FT   REGION          141..155
FT                   /note="Leucine-zipper"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00978"
FT   REGION          189..236
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        26..43
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        61..75
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        196..210
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   MUTAGEN         105
FT                   /note="K->Q: Loss of DNA-binding."
FT   MUTAGEN         106
FT                   /note="R->Q: Loss of DNA-binding."
SQ   SEQUENCE   236 AA;  25895 MW;  88AE35FEF57FEF6A CRC64;
     MSSADFNFGD FTTTYTSPTI PAYPDTLGQL QPIQPNPQAA YPPVSQHHAS HHVQHPHQPG
     YVLSNPPQLS GNKRKASDAM SVPPTPGARV MSFEEASRLA AEEDKRKRNT AASARFRIKK
     KQREQALEKS AKEMSEKVTQ LEGRIQALET ENKWLKGLVT EKHGSKEDIL KLLREFSAHA
     AKVSKDAAAA AADKAEAAAD KADAERAREE SSFCVSTSSP SSDESVDTDN KKRRKD
 
 
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