ID   CYSA_CUCSA              Reviewed;         350 AA.
AC   Q9G4F5;
DT   22-AUG-2003, integrated into UniProtKB/Swiss-Prot.
DT   01-MAR-2001, sequence version 1.
DT   03-AUG-2022, entry version 96.
DE   RecName: Full=Sulfate/thiosulfate import ATP-binding protein cysA;
DE            EC=7.3.2.3;
DE   AltName: Full=Sulfate-transporting ATPase;
GN   Name=CYSA;
OS   Cucumis sativus (Cucumber).
OG   Mitochondrion.
OG   Plasmid pC1.
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   rosids; fabids; Cucurbitales; Cucurbitaceae; Benincaseae; Cucumis.
OX   NCBI_TaxID=3659;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=cv. Jinyan 4;
RX   PubMed=11192436;
RA   Li J., Wen Y., Gao C.;
RT   "Properties and nucleotide sequence of mitochondrial plasmid-like DNA pC1
RT   of Cucumber.";
RL   Yi Chuan Xue Bao 27:911-917(2000).
CC   -!- FUNCTION: Part of the ABC transporter complex involved in
CC       sulfate/thiosulfate import. Responsible for energy coupling to the
CC       transport system (By similarity). {ECO:0000250}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=ATP + H2O + sulfate(out) = ADP + H(+) + phosphate +
CC         sulfate(in); Xref=Rhea:RHEA:10192, ChEBI:CHEBI:15377,
CC         ChEBI:CHEBI:15378, ChEBI:CHEBI:16189, ChEBI:CHEBI:30616,
CC         ChEBI:CHEBI:43474, ChEBI:CHEBI:456216; EC=7.3.2.3;
CC         Evidence={ECO:0000305};
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=ATP + H2O + thiosulfate(out) = ADP + H(+) + phosphate +
CC         thiosulfate(in); Xref=Rhea:RHEA:29871, ChEBI:CHEBI:15377,
CC         ChEBI:CHEBI:15378, ChEBI:CHEBI:30616, ChEBI:CHEBI:33542,
CC         ChEBI:CHEBI:43474, ChEBI:CHEBI:456216; EC=7.3.2.3;
CC         Evidence={ECO:0000305};
CC   -!- SUBCELLULAR LOCATION: Mitochondrion {ECO:0000305}.
CC   -!- SIMILARITY: Belongs to the ABC transporter superfamily.
CC       Sulfate/tungstate importer (TC 3.A.1.6) family. {ECO:0000305}.
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DR   EMBL; AF285185; AAG41904.1; -; Genomic_DNA.
DR   AlphaFoldDB; Q9G4F5; -.
DR   SMR; Q9G4F5; -.
DR   GO; GO:0043190; C:ATP-binding cassette (ABC) transporter complex; IEA:InterPro.
DR   GO; GO:0005739; C:mitochondrion; IEA:UniProtKB-SubCell.
DR   GO; GO:0015419; F:ABC-type sulfate transporter activity; IEA:UniProtKB-EC.
DR   GO; GO:0102025; F:ABC-type thiosulfate transporter activity; IEA:UniProtKB-EC.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR   CDD; cd03296; ABC_CysA_sulfate_importer; 1.
DR   Gene3D; 3.40.50.300; -; 1.
DR   InterPro; IPR003593; AAA+_ATPase.
DR   InterPro; IPR003439; ABC_transporter-like_ATP-bd.
DR   InterPro; IPR017871; ABC_transporter-like_CS.
DR   InterPro; IPR041193; CysA_C.
DR   InterPro; IPR027417; P-loop_NTPase.
DR   InterPro; IPR005666; Sulph_transpt1.
DR   Pfam; PF00005; ABC_tran; 1.
DR   Pfam; PF17850; CysA_C_terminal; 1.
DR   SMART; SM00382; AAA; 1.
DR   SUPFAM; SSF52540; SSF52540; 1.
DR   TIGRFAMs; TIGR00968; 3a0106s01; 1.
DR   PROSITE; PS00211; ABC_TRANSPORTER_1; 1.
DR   PROSITE; PS50893; ABC_TRANSPORTER_2; 1.
DR   PROSITE; PS51237; CYSA; 1.
PE   3: Inferred from homology;
KW   ATP-binding; Mitochondrion; Nucleotide-binding; Plasmid; Sulfate transport;
KW   Translocase; Transport.
FT   CHAIN           1..350
FT                   /note="Sulfate/thiosulfate import ATP-binding protein cysA"
FT                   /id="PRO_0000092311"
FT   DOMAIN          3..237
FT                   /note="ABC transporter"
FT   BINDING         35..42
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   350 AA;  39313 MW;  C2AF4A75ED93012B CRC64;
     MSIEVRNLSK RFGQFRALND VSLHIETGEL VALLGPSGCG KTTLLRIIAG LESADNGSVL
     FAGEDATSVD VRQRQVGFVF QHYALFKHMT VFENVAFGLR VKHRSQRPSE DQIQRKVHDL
     LGLVQLDWLA DRYPAQLSGG QRQRIALARA LAVEPRVLLL DEPFGALDAK VRKELRRWLR
     RLHDELNVAS VFVTHDQEEA LEVADRVVLM NAGRIEQVGT PREVWEGPAT PFVYGFLGDV
     NQLQGVASRG VWEGAGLSLP APELAQAENQ RATAYVRPHE FLYTPAAQEI IAKNYYRPID
     KTVAAKYESK FPKVKLVTID DKIFGGWRKA QKDHFSDGGT FDQIYQPQKK