CYSA_XANCP
ID CYSA_XANCP Reviewed; 343 AA.
AC Q8PC11;
DT 22-AUG-2003, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-2002, sequence version 1.
DT 03-AUG-2022, entry version 126.
DE RecName: Full=Sulfate/thiosulfate import ATP-binding protein CysA {ECO:0000255|HAMAP-Rule:MF_01701};
DE EC=7.3.2.3 {ECO:0000255|HAMAP-Rule:MF_01701};
DE AltName: Full=Sulfate-transporting ATPase {ECO:0000255|HAMAP-Rule:MF_01701};
GN Name=cysA {ECO:0000255|HAMAP-Rule:MF_01701}; OrderedLocusNames=XCC0943;
OS Xanthomonas campestris pv. campestris (strain ATCC 33913 / DSM 3586 / NCPPB
OS 528 / LMG 568 / P 25).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Xanthomonadales;
OC Xanthomonadaceae; Xanthomonas.
OX NCBI_TaxID=190485;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 33913 / DSM 3586 / NCPPB 528 / LMG 568 / P 25;
RX PubMed=12024217; DOI=10.1038/417459a;
RA da Silva A.C.R., Ferro J.A., Reinach F.C., Farah C.S., Furlan L.R.,
RA Quaggio R.B., Monteiro-Vitorello C.B., Van Sluys M.A., Almeida N.F. Jr.,
RA Alves L.M.C., do Amaral A.M., Bertolini M.C., Camargo L.E.A., Camarotte G.,
RA Cannavan F., Cardozo J., Chambergo F., Ciapina L.P., Cicarelli R.M.B.,
RA Coutinho L.L., Cursino-Santos J.R., El-Dorry H., Faria J.B.,
RA Ferreira A.J.S., Ferreira R.C.C., Ferro M.I.T., Formighieri E.F.,
RA Franco M.C., Greggio C.C., Gruber A., Katsuyama A.M., Kishi L.T.,
RA Leite R.P., Lemos E.G.M., Lemos M.V.F., Locali E.C., Machado M.A.,
RA Madeira A.M.B.N., Martinez-Rossi N.M., Martins E.C., Meidanis J.,
RA Menck C.F.M., Miyaki C.Y., Moon D.H., Moreira L.M., Novo M.T.M.,
RA Okura V.K., Oliveira M.C., Oliveira V.R., Pereira H.A., Rossi A.,
RA Sena J.A.D., Silva C., de Souza R.F., Spinola L.A.F., Takita M.A.,
RA Tamura R.E., Teixeira E.C., Tezza R.I.D., Trindade dos Santos M.,
RA Truffi D., Tsai S.M., White F.F., Setubal J.C., Kitajima J.P.;
RT "Comparison of the genomes of two Xanthomonas pathogens with differing host
RT specificities.";
RL Nature 417:459-463(2002).
CC -!- FUNCTION: Part of the ABC transporter complex CysAWTP involved in
CC sulfate/thiosulfate import. Responsible for energy coupling to the
CC transport system. {ECO:0000255|HAMAP-Rule:MF_01701}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + H2O + sulfate(out) = ADP + H(+) + phosphate +
CC sulfate(in); Xref=Rhea:RHEA:10192, ChEBI:CHEBI:15377,
CC ChEBI:CHEBI:15378, ChEBI:CHEBI:16189, ChEBI:CHEBI:30616,
CC ChEBI:CHEBI:43474, ChEBI:CHEBI:456216; EC=7.3.2.3;
CC Evidence={ECO:0000255|HAMAP-Rule:MF_01701};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + H2O + thiosulfate(out) = ADP + H(+) + phosphate +
CC thiosulfate(in); Xref=Rhea:RHEA:29871, ChEBI:CHEBI:15377,
CC ChEBI:CHEBI:15378, ChEBI:CHEBI:30616, ChEBI:CHEBI:33542,
CC ChEBI:CHEBI:43474, ChEBI:CHEBI:456216; EC=7.3.2.3;
CC Evidence={ECO:0000255|HAMAP-Rule:MF_01701};
CC -!- SUBUNIT: The complex is composed of two ATP-binding proteins (CysA),
CC two transmembrane proteins (CysT and CysW) and a solute-binding protein
CC (CysP). {ECO:0000255|HAMAP-Rule:MF_01701}.
CC -!- SUBCELLULAR LOCATION: Cell inner membrane {ECO:0000255|HAMAP-
CC Rule:MF_01701}; Peripheral membrane protein {ECO:0000255|HAMAP-
CC Rule:MF_01701}.
CC -!- SIMILARITY: Belongs to the ABC transporter superfamily.
CC Sulfate/tungstate importer (TC 3.A.1.6) family. {ECO:0000255|HAMAP-
CC Rule:MF_01701}.
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DR EMBL; AE008922; AAM40253.1; -; Genomic_DNA.
DR RefSeq; NP_636329.1; NC_003902.1.
DR RefSeq; WP_011036157.1; NC_003902.1.
DR AlphaFoldDB; Q8PC11; -.
DR SMR; Q8PC11; -.
DR STRING; 340.xcc-b100_3410; -.
DR EnsemblBacteria; AAM40253; AAM40253; XCC0943.
DR GeneID; 58014481; -.
DR KEGG; xcc:XCC0943; -.
DR PATRIC; fig|190485.4.peg.1016; -.
DR eggNOG; COG1118; Bacteria.
DR HOGENOM; CLU_000604_1_1_6; -.
DR OMA; HLGWEIQ; -.
DR Proteomes; UP000001010; Chromosome.
DR GO; GO:0043190; C:ATP-binding cassette (ABC) transporter complex; IEA:InterPro.
DR GO; GO:0015419; F:ABC-type sulfate transporter activity; IEA:UniProtKB-EC.
DR GO; GO:0102025; F:ABC-type thiosulfate transporter activity; IEA:UniProtKB-EC.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0008272; P:sulfate transport; IBA:GO_Central.
DR CDD; cd03296; ABC_CysA_sulfate_importer; 1.
DR Gene3D; 3.40.50.300; -; 1.
DR InterPro; IPR003593; AAA+_ATPase.
DR InterPro; IPR003439; ABC_transporter-like_ATP-bd.
DR InterPro; IPR017871; ABC_transporter-like_CS.
DR InterPro; IPR008995; Mo/tungstate-bd_C_term_dom.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR005666; Sulph_transpt1.
DR InterPro; IPR024765; TOBE-like.
DR Pfam; PF00005; ABC_tran; 1.
DR Pfam; PF12857; TOBE_3; 1.
DR SMART; SM00382; AAA; 1.
DR SUPFAM; SSF50331; SSF50331; 1.
DR SUPFAM; SSF52540; SSF52540; 1.
DR TIGRFAMs; TIGR00968; 3a0106s01; 1.
DR PROSITE; PS00211; ABC_TRANSPORTER_1; 1.
DR PROSITE; PS50893; ABC_TRANSPORTER_2; 1.
DR PROSITE; PS51237; CYSA; 1.
PE 3: Inferred from homology;
KW ATP-binding; Cell inner membrane; Cell membrane; Membrane;
KW Nucleotide-binding; Reference proteome; Sulfate transport; Translocase;
KW Transport.
FT CHAIN 1..343
FT /note="Sulfate/thiosulfate import ATP-binding protein CysA"
FT /id="PRO_0000092301"
FT DOMAIN 3..237
FT /note="ABC transporter"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01701"
FT BINDING 35..42
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01701"
SQ SEQUENCE 343 AA; 37761 MW; EF3CF18A74A534B0 CRC64;
MGIRISHLRK QFASFTALDD ITLDVRQGEL LALLGPSGSG KTTLLRIMAG LEHADGGQVL
FGDEDATRMS VQSRRVGFVF QHYALFKHMD VFENIAFGLR VRRGKERWAE ARIRARVEEL
LALVQLQGLE QRYPTQLSGG QRQRVALARA LAIEPRVLLL DEPFGALDAQ VRRDLRRWLR
ELHEQTGLTT VFVTHDQEEA LELADRVAIL NRGRIEQLDT PARVYDTPAS PFVYSFVGAV
NRIPGVLAHG QIQVAGHALP SANPALAAGP VEVYVRPEDL VPDADGWPAT VAWAQRSGAR
LRVRAILDAA GNEVEVELPA SAGTITAGQQ LRLAARHYGV FPA
