ID   CYSB_BURCJ              Reviewed;         313 AA.
AC   B4E8V9;
DT   12-SEP-2018, integrated into UniProtKB/Swiss-Prot.
DT   23-SEP-2008, sequence version 1.
DT   03-AUG-2022, entry version 81.
DE   RecName: Full=HTH-type transcriptional regulator CysB {ECO:0000305};
GN   Name=cysB {ECO:0000303|PubMed:16997956};
GN   ORFNames=BCAL2686 {ECO:0000312|EMBL:CAR52987.1};
OS   Burkholderia cenocepacia (strain ATCC BAA-245 / DSM 16553 / LMG 16656 /
OS   NCTC 13227 / J2315 / CF5610) (Burkholderia cepacia (strain J2315)).
OC   Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
OC   Burkholderiaceae; Burkholderia; Burkholderia cepacia complex.
OX   NCBI_TaxID=216591;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC BAA-245 / DSM 16553 / LMG 16656 / NCTC 13227 / J2315 / CF5610;
RX   PubMed=18931103; DOI=10.1128/jb.01230-08;
RA   Holden M.T., Seth-Smith H.M., Crossman L.C., Sebaihia M., Bentley S.D.,
RA   Cerdeno-Tarraga A.M., Thomson N.R., Bason N., Quail M.A., Sharp S.,
RA   Cherevach I., Churcher C., Goodhead I., Hauser H., Holroyd N., Mungall K.,
RA   Scott P., Walker D., White B., Rose H., Iversen P., Mil-Homens D.,
RA   Rocha E.P., Fialho A.M., Baldwin A., Dowson C., Barrell B.G., Govan J.R.,
RA   Vandamme P., Hart C.A., Mahenthiralingam E., Parkhill J.;
RT   "The genome of Burkholderia cenocepacia J2315, an epidemic pathogen of
RT   cystic fibrosis patients.";
RL   J. Bacteriol. 191:261-277(2009).
RN   [2]
RP   FUNCTION, DNA-BINDING, INDUCTION, AND DISRUPTION PHENOTYPE.
RC   STRAIN=715j;
RX   PubMed=16997956; DOI=10.1128/jb.00592-06;
RA   Iwanicka-Nowicka R., Zielak A., Cook A.M., Thomas M.S., Hryniewicz M.M.;
RT   "Regulation of sulfur assimilation pathways in Burkholderia cenocepacia:
RT   identification of transcription factors CysB and SsuR and their role in
RT   control of target genes.";
RL   J. Bacteriol. 189:1675-1688(2007).
CC   -!- FUNCTION: Transcriptional regulator preferentially involved in the
CC       control of sulfate transport and reduction. Binds to DNA at target
CC       promoter regions. {ECO:0000269|PubMed:16997956}.
CC   -!- INDUCTION: Negatively controlled by both CysB and SsuR.
CC       {ECO:0000269|PubMed:16997956}.
CC   -!- DISRUPTION PHENOTYPE: Mutant is not able to utilize sulfate, sulfite
CC       and ethanesulfonate for cysteine biosynthesis.
CC       {ECO:0000269|PubMed:16997956}.
CC   -!- SIMILARITY: Belongs to the LysR transcriptional regulatory family.
CC       {ECO:0000305}.
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DR   EMBL; AM747720; CAR52987.1; -; Genomic_DNA.
DR   RefSeq; WP_006478072.1; NC_011000.1.
DR   AlphaFoldDB; B4E8V9; -.
DR   SMR; B4E8V9; -.
DR   STRING; 216591.BCAL2686; -.
DR   EnsemblBacteria; CAR52987; CAR52987; BCAL2686.
DR   GeneID; 56559063; -.
DR   KEGG; bcj:BCAL2686; -.
DR   eggNOG; COG0583; Bacteria.
DR   HOGENOM; CLU_039613_6_2_4; -.
DR   OMA; ITYDNAF; -.
DR   OrthoDB; 990666at2; -.
DR   BioCyc; BCEN216591:G1G1V-2974-MON; -.
DR   Proteomes; UP000001035; Chromosome 1.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0003700; F:DNA-binding transcription factor activity; IEA:InterPro.
DR   CDD; cd08413; PBP2_CysB_like; 1.
DR   Gene3D; 1.10.10.10; -; 1.
DR   InterPro; IPR037423; CysB_PBP2.
DR   InterPro; IPR005119; LysR_subst-bd.
DR   InterPro; IPR000847; Tscrpt_reg_HTH_LysR.
DR   InterPro; IPR036388; WH-like_DNA-bd_sf.
DR   InterPro; IPR036390; WH_DNA-bd_sf.
DR   Pfam; PF00126; HTH_1; 1.
DR   Pfam; PF03466; LysR_substrate; 1.
DR   PRINTS; PR00039; HTHLYSR.
DR   SUPFAM; SSF46785; SSF46785; 1.
DR   PROSITE; PS50931; HTH_LYSR; 1.
PE   1: Evidence at protein level;
KW   DNA-binding; Transcription; Transcription regulation.
FT   CHAIN           1..313
FT                   /note="HTH-type transcriptional regulator CysB"
FT                   /id="PRO_0000445022"
FT   DOMAIN          1..59
FT                   /note="HTH lysR-type"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00253"
FT   DNA_BIND        19..38
FT                   /note="H-T-H motif"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00253"
SQ   SEQUENCE   313 AA;  34874 MW;  2C06E0ED29B088D5 CRC64;
     MNLHQFRFVR EAVRQNFNLT EAAKALYTSQ PGVSKAIIEL EDELGVEIFT RHGKRVRSLT
     EPGRIILASV ERILQEVESL KRVGKDYAAQ DQGNLTIAAT HTQARYSLPA AIAEFKKRFP
     KVHLSILQGS PTQVAEMVIH DQADLAIATE AISDYKELVS LPCFQWHHAA VVPADHPLLE
     RKPVTLDDLA QYPLITYDDA FAGRKKINHA FALRGLSPDI VLEAIDADVI KTYVELGLGV
     GIMADIAFNP ERDRGLRLIP VGHLFGSNVT RVALKQGAYL RSYVYTLVEL LSPTLNRKLI
     EQALKGEAES YEL