CYSB_ECOLI
ID CYSB_ECOLI Reviewed; 324 AA.
AC P0A9F3; P06613; P76834;
DT 01-JAN-1988, integrated into UniProtKB/Swiss-Prot.
DT 01-JAN-1988, sequence version 1.
DT 03-AUG-2022, entry version 125.
DE RecName: Full=HTH-type transcriptional regulator CysB;
DE AltName: Full=Cys regulon transcriptional activator;
GN Name=cysB; OrderedLocusNames=b1275, JW1267;
OS Escherichia coli (strain K12).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC Enterobacteriaceae; Escherichia.
OX NCBI_TaxID=83333;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=3032952; DOI=10.1016/s0021-9258(18)45528-7;
RA Ostrowski J., Jagura-Burdzy G., Kredich N.M.;
RT "DNA sequences of the cysB regions of Salmonella typhimurium and
RT Escherichia coli.";
RL J. Biol. Chem. 262:5999-6005(1987).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=K12;
RX PubMed=2182030; DOI=10.1016/0006-291x(90)90617-v;
RA Tei H., Watanabe K., Murata K., Kimura A.;
RT "Analysis of the Escherichia coli K-12 cysB gene and its product using the
RT method of gene fusion.";
RL Biochem. Biophys. Res. Commun. 167:962-969(1990).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX PubMed=9097039; DOI=10.1093/dnares/3.6.363;
RA Aiba H., Baba T., Fujita K., Hayashi K., Inada T., Isono K., Itoh T.,
RA Kasai H., Kashimoto K., Kimura S., Kitakawa M., Kitagawa M., Makino K.,
RA Miki T., Mizobuchi K., Mori H., Mori T., Motomura K., Nakade S.,
RA Nakamura Y., Nashimoto H., Nishio Y., Oshima T., Saito N., Sampei G.,
RA Seki Y., Sivasundaram S., Tagami H., Takeda J., Takemoto K., Takeuchi Y.,
RA Wada C., Yamamoto Y., Horiuchi T.;
RT "A 570-kb DNA sequence of the Escherichia coli K-12 genome corresponding to
RT the 28.0-40.1 min region on the linkage map.";
RL DNA Res. 3:363-377(1996).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / MG1655 / ATCC 47076;
RX PubMed=9278503; DOI=10.1126/science.277.5331.1453;
RA Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V.,
RA Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F.,
RA Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B.,
RA Shao Y.;
RT "The complete genome sequence of Escherichia coli K-12.";
RL Science 277:1453-1462(1997).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX PubMed=16738553; DOI=10.1038/msb4100049;
RA Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S.,
RA Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.;
RT "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655
RT and W3110.";
RL Mol. Syst. Biol. 2:E1-E5(2006).
RN [6]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 249-324.
RX PubMed=1541275; DOI=10.1111/j.1432-1033.1992.tb16673.x;
RA Prodromou C., Artymiuk P.J., Guest J.R.;
RT "The aconitase of Escherichia coli. Nucleotide sequence of the aconitase
RT gene and amino acid sequence similarity with mitochondrial aconitases, the
RT iron-responsive-element-binding protein and isopropylmalate isomerases.";
RL Eur. J. Biochem. 204:599-609(1992).
CC -!- FUNCTION: This protein is a positive regulator of gene expression for
CC the cysteine regulon, a system of 10 or more loci involved in the
CC biosynthesis of L-cysteine from inorganic sulfate. The inducer for CysB
CC is N-acetylserine. CysB inhibits its own transcription.
CC -!- SUBUNIT: Homotetramer. {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm.
CC -!- SIMILARITY: Belongs to the LysR transcriptional regulatory family.
CC {ECO:0000305}.
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DR EMBL; M15041; AAA23642.1; -; Genomic_DNA.
DR EMBL; M34332; AAA23643.1; -; Genomic_DNA.
DR EMBL; U00096; AAC74357.1; -; Genomic_DNA.
DR EMBL; AP009048; BAA14827.1; -; Genomic_DNA.
DR EMBL; X60293; CAA42833.1; -; Genomic_DNA.
DR PIR; A26695; RGECCB.
DR RefSeq; NP_415791.1; NC_000913.3.
DR RefSeq; WP_000776253.1; NZ_STEB01000005.1.
DR AlphaFoldDB; P0A9F3; -.
DR SMR; P0A9F3; -.
DR BioGRID; 4260138; 28.
DR BioGRID; 850138; 3.
DR DIP; DIP-36048N; -.
DR IntAct; P0A9F3; 10.
DR STRING; 511145.b1275; -.
DR jPOST; P0A9F3; -.
DR PaxDb; P0A9F3; -.
DR PRIDE; P0A9F3; -.
DR EnsemblBacteria; AAC74357; AAC74357; b1275.
DR EnsemblBacteria; BAA14827; BAA14827; BAA14827.
DR GeneID; 66674903; -.
DR GeneID; 945771; -.
DR KEGG; ecj:JW1267; -.
DR KEGG; eco:b1275; -.
DR PATRIC; fig|1411691.4.peg.1009; -.
DR EchoBASE; EB0181; -.
DR eggNOG; COG0583; Bacteria.
DR HOGENOM; CLU_039613_6_2_6; -.
DR InParanoid; P0A9F3; -.
DR OMA; FLRTYMY; -.
DR PhylomeDB; P0A9F3; -.
DR BioCyc; EcoCyc:PD00232; -.
DR BioCyc; MetaCyc:PD00232; -.
DR PRO; PR:P0A9F3; -.
DR Proteomes; UP000000318; Chromosome.
DR Proteomes; UP000000625; Chromosome.
DR GO; GO:0005829; C:cytosol; IDA:EcoCyc.
DR GO; GO:0003677; F:DNA binding; IDA:EcoCyc.
DR GO; GO:0003700; F:DNA-binding transcription factor activity; IEA:InterPro.
DR GO; GO:0000976; F:transcription cis-regulatory region binding; IBA:GO_Central.
DR GO; GO:0019344; P:cysteine biosynthetic process; IBA:GO_Central.
DR GO; GO:0006355; P:regulation of transcription, DNA-templated; IBA:GO_Central.
DR GO; GO:0010165; P:response to X-ray; IMP:EcoCyc.
DR Gene3D; 1.10.10.10; -; 1.
DR InterPro; IPR005119; LysR_subst-bd.
DR InterPro; IPR000847; Tscrpt_reg_HTH_LysR.
DR InterPro; IPR036388; WH-like_DNA-bd_sf.
DR InterPro; IPR036390; WH_DNA-bd_sf.
DR Pfam; PF00126; HTH_1; 1.
DR Pfam; PF03466; LysR_substrate; 1.
DR PRINTS; PR00039; HTHLYSR.
DR SUPFAM; SSF46785; SSF46785; 1.
DR PROSITE; PS50931; HTH_LYSR; 1.
PE 3: Inferred from homology;
KW Activator; Amino-acid biosynthesis; Cysteine biosynthesis; Cytoplasm;
KW DNA-binding; Reference proteome; Transcription; Transcription regulation.
FT CHAIN 1..324
FT /note="HTH-type transcriptional regulator CysB"
FT /id="PRO_0000105613"
FT DOMAIN 1..59
FT /note="HTH lysR-type"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00253"
FT DNA_BIND 19..38
FT /note="H-T-H motif"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00253"
SQ SEQUENCE 324 AA; 36150 MW; 3B35E9CFEF6F4C84 CRC64;
MKLQQLRYIV EVVNHNLNVS STAEGLYTSQ PGISKQVRML EDELGIQIFS RSGKHLTQVT
PAGQEIIRIA REVLSKVDAI KSVAGEHTWP DKGSLYIATT HTQARYALPN VIKGFIERYP
RVSLHMHQGS PTQIADAVSK GNADFAIATE ALHLYEDLVM LPCYHWNRAI VVTPDHPLAG
KKAITIEELA QYPLVTYTFG FTGRSELDTA FNRAGLTPRI VFTATDADVI KTYVRLGLGV
GVIASMAVDP VADPDLVRVD AHDIFSHSTT KIGFRRSTFL RSYMYDFIQR FAPHLTRDVV
DAAVALRSNE EIEVMFKDIK LPEK
