CYSB_SALTY
ID CYSB_SALTY Reviewed; 324 AA.
AC P06614;
DT 01-JAN-1988, integrated into UniProtKB/Swiss-Prot.
DT 01-JAN-1988, sequence version 1.
DT 03-AUG-2022, entry version 142.
DE RecName: Full=HTH-type transcriptional regulator CysB;
DE AltName: Full=Cys regulon transcriptional activator;
GN Name=cysB; OrderedLocusNames=STM1713;
OS Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC Enterobacteriaceae; Salmonella.
OX NCBI_TaxID=99287;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=3032952; DOI=10.1016/s0021-9258(18)45528-7;
RA Ostrowski J., Jagura-Burdzy G., Kredich N.M.;
RT "DNA sequences of the cysB regions of Salmonella typhimurium and
RT Escherichia coli.";
RL J. Biol. Chem. 262:5999-6005(1987).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=LT2 / SGSC1412 / ATCC 700720;
RX PubMed=11677609; DOI=10.1038/35101614;
RA McClelland M., Sanderson K.E., Spieth J., Clifton S.W., Latreille P.,
RA Courtney L., Porwollik S., Ali J., Dante M., Du F., Hou S., Layman D.,
RA Leonard S., Nguyen C., Scott K., Holmes A., Grewal N., Mulvaney E.,
RA Ryan E., Sun H., Florea L., Miller W., Stoneking T., Nhan M., Waterston R.,
RA Wilson R.K.;
RT "Complete genome sequence of Salmonella enterica serovar Typhimurium LT2.";
RL Nature 413:852-856(2001).
RN [3]
RP PARTIAL PROTEIN SEQUENCE, AND CHARACTERIZATION.
RX PubMed=3032953; DOI=10.1016/s0021-9258(18)45529-9;
RA Miller B.E., Kredich N.M.;
RT "Purification of the cysB protein from Salmonella typhimurium.";
RL J. Biol. Chem. 262:6006-6009(1987).
CC -!- FUNCTION: This protein is a positive regulator of gene expression for
CC the cysteine regulon, a system of 10 or more loci involved in the
CC biosynthesis of L-cysteine from inorganic sulfate. The inducer for CysB
CC is N-acetylserine. CysB inhibits its own transcription.
CC -!- SUBUNIT: Homotetramer.
CC -!- SUBCELLULAR LOCATION: Cytoplasm.
CC -!- SIMILARITY: Belongs to the LysR transcriptional regulatory family.
CC {ECO:0000305}.
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DR EMBL; M15040; AAA27045.1; -; Genomic_DNA.
DR EMBL; AE006468; AAL20631.1; -; Genomic_DNA.
DR PIR; B26695; RGEBCB.
DR RefSeq; NP_460672.1; NC_003197.2.
DR RefSeq; WP_000776233.1; NC_003197.2.
DR PDB; 4GWO; X-ray; 2.39 A; A/B=1-324.
DR PDB; 4GXA; X-ray; 2.81 A; A/B=86-324.
DR PDB; 4LON; X-ray; 2.20 A; B=86-324.
DR PDB; 4LP2; X-ray; 2.30 A; B=86-324.
DR PDB; 4LQ2; X-ray; 2.69 A; A=86-324.
DR PDB; 4LQ5; X-ray; 2.80 A; A=86-324.
DR PDB; 4M4G; X-ray; 2.70 A; A=86-324.
DR PDBsum; 4GWO; -.
DR PDBsum; 4GXA; -.
DR PDBsum; 4LON; -.
DR PDBsum; 4LP2; -.
DR PDBsum; 4LQ2; -.
DR PDBsum; 4LQ5; -.
DR PDBsum; 4M4G; -.
DR AlphaFoldDB; P06614; -.
DR SMR; P06614; -.
DR STRING; 99287.STM1713; -.
DR PaxDb; P06614; -.
DR EnsemblBacteria; AAL20631; AAL20631; STM1713.
DR GeneID; 1253232; -.
DR KEGG; stm:STM1713; -.
DR PATRIC; fig|99287.12.peg.1810; -.
DR HOGENOM; CLU_039613_6_2_6; -.
DR OMA; FLRTYMY; -.
DR PhylomeDB; P06614; -.
DR BioCyc; SENT99287:STM1713-MON; -.
DR Proteomes; UP000001014; Chromosome.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0003700; F:DNA-binding transcription factor activity; IEA:InterPro.
DR GO; GO:0000976; F:transcription cis-regulatory region binding; IBA:GO_Central.
DR GO; GO:0019344; P:cysteine biosynthetic process; IBA:GO_Central.
DR GO; GO:0006355; P:regulation of transcription, DNA-templated; IBA:GO_Central.
DR Gene3D; 1.10.10.10; -; 1.
DR InterPro; IPR005119; LysR_subst-bd.
DR InterPro; IPR000847; Tscrpt_reg_HTH_LysR.
DR InterPro; IPR036388; WH-like_DNA-bd_sf.
DR InterPro; IPR036390; WH_DNA-bd_sf.
DR Pfam; PF00126; HTH_1; 1.
DR Pfam; PF03466; LysR_substrate; 1.
DR PRINTS; PR00039; HTHLYSR.
DR SUPFAM; SSF46785; SSF46785; 1.
DR PROSITE; PS50931; HTH_LYSR; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Activator; Amino-acid biosynthesis; Cysteine biosynthesis;
KW Cytoplasm; Direct protein sequencing; DNA-binding; Reference proteome;
KW Transcription; Transcription regulation.
FT CHAIN 1..324
FT /note="HTH-type transcriptional regulator CysB"
FT /id="PRO_0000105616"
FT DOMAIN 1..59
FT /note="HTH lysR-type"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00253"
FT DNA_BIND 19..38
FT /note="H-T-H motif"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00253"
FT STRAND 93..99
FT /evidence="ECO:0007829|PDB:4LON"
FT HELIX 101..106
FT /evidence="ECO:0007829|PDB:4LON"
FT HELIX 109..118
FT /evidence="ECO:0007829|PDB:4LON"
FT STRAND 122..128
FT /evidence="ECO:0007829|PDB:4LON"
FT HELIX 131..139
FT /evidence="ECO:0007829|PDB:4LON"
FT STRAND 144..149
FT /evidence="ECO:0007829|PDB:4LON"
FT HELIX 152..154
FT /evidence="ECO:0007829|PDB:4GWO"
FT STRAND 158..166
FT /evidence="ECO:0007829|PDB:4LON"
FT STRAND 168..172
FT /evidence="ECO:0007829|PDB:4LON"
FT HELIX 177..180
FT /evidence="ECO:0007829|PDB:4LON"
FT HELIX 186..189
FT /evidence="ECO:0007829|PDB:4LON"
FT STRAND 192..197
FT /evidence="ECO:0007829|PDB:4LON"
FT STRAND 201..203
FT /evidence="ECO:0007829|PDB:4LQ2"
FT HELIX 204..213
FT /evidence="ECO:0007829|PDB:4LON"
FT STRAND 219..225
FT /evidence="ECO:0007829|PDB:4LON"
FT HELIX 227..234
FT /evidence="ECO:0007829|PDB:4LON"
FT TURN 235..237
FT /evidence="ECO:0007829|PDB:4LON"
FT STRAND 240..244
FT /evidence="ECO:0007829|PDB:4LON"
FT HELIX 245..247
FT /evidence="ECO:0007829|PDB:4LON"
FT TURN 250..252
FT /evidence="ECO:0007829|PDB:4LON"
FT STRAND 257..260
FT /evidence="ECO:0007829|PDB:4LON"
FT TURN 262..264
FT /evidence="ECO:0007829|PDB:4LON"
FT STRAND 268..274
FT /evidence="ECO:0007829|PDB:4LON"
FT STRAND 276..278
FT /evidence="ECO:0007829|PDB:4LQ5"
FT HELIX 282..291
FT /evidence="ECO:0007829|PDB:4LON"
FT HELIX 297..306
FT /evidence="ECO:0007829|PDB:4LON"
FT HELIX 309..315
FT /evidence="ECO:0007829|PDB:4LON"
FT TURN 316..318
FT /evidence="ECO:0007829|PDB:4LON"
SQ SEQUENCE 324 AA; 36013 MW; CCECF585A6851D34 CRC64;
MKLQQLRYIV EVVNHNLNVS STAEGLYTSQ PGISKQVRML EDELGIQIFA RSGKHLTQVT
PAGQEIIRIA REVLSKVDAI KSVAGEHTWP DKGSLYIATT HTQARYALPG VIKGFIERYP
RVSLHMHQGS PTQIAEAVSK GNADFAIATE ALHLYDDLVM LPCYHWNRSI VVTPDHPLAA
TSSVTIEALA QYPLVTYTFG FTGRSELDTA FNRAGLTPRI VFTATDADVI KTYVRLGLGV
GVIASMAVDP LADPDLVRID AHDIFSHSTT KIGFRRSTFL RSYMYDFIQR FAPHLTRDVV
DTAVALRSNE EIEAMFQDIK LPEK
