ID   ACSB_DICDI              Reviewed;         688 AA.
AC   Q554Z5; Q869S4;
DT   08-APR-2008, integrated into UniProtKB/Swiss-Prot.
DT   24-MAY-2005, sequence version 1.
DT   03-AUG-2022, entry version 83.
DE   RecName: Full=Acyl-CoA synthetase short-chain family member B, mitochondrial;
DE            EC=6.2.1.1;
DE   Flags: Precursor;
GN   Name=aslB; ORFNames=DDB_G0274939;
OS   Dictyostelium discoideum (Slime mold).
OC   Eukaryota; Amoebozoa; Evosea; Eumycetozoa; Dictyostelia; Dictyosteliales;
OC   Dictyosteliaceae; Dictyostelium.
OX   NCBI_TaxID=44689;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=AX4;
RX   PubMed=12097910; DOI=10.1038/nature00847;
RA   Gloeckner G., Eichinger L., Szafranski K., Pachebat J.A., Bankier A.T.,
RA   Dear P.H., Lehmann R., Baumgart C., Parra G., Abril J.F., Guigo R.,
RA   Kumpf K., Tunggal B., Cox E.C., Quail M.A., Platzer M., Rosenthal A.,
RA   Noegel A.A.;
RT   "Sequence and analysis of chromosome 2 of Dictyostelium discoideum.";
RL   Nature 418:79-85(2002).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=AX4;
RX   PubMed=15875012; DOI=10.1038/nature03481;
RA   Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R.,
RA   Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B.,
RA   Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T.,
RA   Lehmann R., Hamlin N., Davies R., Gaudet P., Fey P., Pilcher K., Chen G.,
RA   Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N.,
RA   Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E.,
RA   Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N.,
RA   Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D.,
RA   Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T.,
RA   Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D.,
RA   Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A.,
RA   Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M.,
RA   Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A.,
RA   Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y.,
RA   Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C.,
RA   Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R.,
RA   Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.;
RT   "The genome of the social amoeba Dictyostelium discoideum.";
RL   Nature 435:43-57(2005).
CC   -!- FUNCTION: Activates acetate so that it can be used for lipid synthesis
CC       or for energy generation. {ECO:0000250}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=acetate + ATP + CoA = acetyl-CoA + AMP + diphosphate;
CC         Xref=Rhea:RHEA:23176, ChEBI:CHEBI:30089, ChEBI:CHEBI:30616,
CC         ChEBI:CHEBI:33019, ChEBI:CHEBI:57287, ChEBI:CHEBI:57288,
CC         ChEBI:CHEBI:456215; EC=6.2.1.1;
CC   -!- SUBCELLULAR LOCATION: Mitochondrion {ECO:0000305}.
CC   -!- SIMILARITY: Belongs to the ATP-dependent AMP-binding enzyme family.
CC       {ECO:0000305}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; AAFI02000012; EAL70362.1; -; Genomic_DNA.
DR   RefSeq; XP_644178.1; XM_639086.1.
DR   AlphaFoldDB; Q554Z5; -.
DR   SMR; Q554Z5; -.
DR   STRING; 44689.DDB0231689; -.
DR   PaxDb; Q554Z5; -.
DR   EnsemblProtists; EAL70362; EAL70362; DDB_G0274939.
DR   GeneID; 8619607; -.
DR   KEGG; ddi:DDB_G0274939; -.
DR   dictyBase; DDB_G0274939; aslB.
DR   eggNOG; KOG1175; Eukaryota.
DR   HOGENOM; CLU_000022_3_5_1; -.
DR   InParanoid; Q554Z5; -.
DR   OMA; YVFVMGR; -.
DR   PhylomeDB; Q554Z5; -.
DR   Reactome; R-DDI-77111; Synthesis of Ketone Bodies.
DR   PRO; PR:Q554Z5; -.
DR   Proteomes; UP000002195; Chromosome 2.
DR   GO; GO:0005739; C:mitochondrion; IEA:UniProtKB-SubCell.
DR   GO; GO:0003987; F:acetate-CoA ligase activity; ISS:UniProtKB.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR   GO; GO:0050218; F:propionate-CoA ligase activity; IBA:GO_Central.
DR   GO; GO:0008610; P:lipid biosynthetic process; ISS:UniProtKB.
DR   Gene3D; 3.30.300.30; -; 1.
DR   Gene3D; 3.40.50.12780; -; 1.
DR   InterPro; IPR032387; ACAS_N.
DR   InterPro; IPR025110; AMP-bd_C.
DR   InterPro; IPR045851; AMP-bd_C_sf.
DR   InterPro; IPR020845; AMP-binding_CS.
DR   InterPro; IPR000873; AMP-dep_Synth/Lig.
DR   InterPro; IPR042099; ANL_N_sf.
DR   Pfam; PF16177; ACAS_N; 1.
DR   Pfam; PF00501; AMP-binding; 1.
DR   Pfam; PF13193; AMP-binding_C; 1.
DR   PROSITE; PS00455; AMP_BINDING; 1.
PE   3: Inferred from homology;
KW   ATP-binding; Ligase; Mitochondrion; Nucleotide-binding; Reference proteome;
KW   Transit peptide.
FT   TRANSIT         1..?
FT                   /note="Mitochondrion"
FT                   /evidence="ECO:0000255"
FT   CHAIN           ?..688
FT                   /note="Acyl-CoA synthetase short-chain family member B,
FT                   mitochondrial"
FT                   /id="PRO_0000327600"
SQ   SEQUENCE   688 AA;  77120 MW;  B79E17A745EC4E02 CRC64;
     MFSNGLSKLI KSKIASTSII TTKNNYSISK LSFSSTSILL NKKYKLSEPF NYEQDCEYAL
     KEPIQFWDEV ASKYVHWNKR YEKVYSGDEY NPEWFKGGVL NACYNALDVH AKDPITKNRI
     AIIHETPSKN NTNKLTYGEL WDEVCIFARG LHNLGVEKGD RVVIYMPMIN QALIAMLACA
     RLGATHSVVF GGFASPQLAQ RIEHFKPKVV ISANFGVEGH KINCYTPLLS KALELSSHKP
     NHTIVYNRLD VKLDAGEVLP PRVEGSLDWS ELIKNIAPYR DYALVDSTHP LYILYTSGTT
     GMPKGVVRDT GGYSVALNYS IRNCYGMKSG DTFFAGSDVG WVVGHTLSVY GPLMVGLTSI
     IFEGKPTVPD ASTYWKLIEK HRVNALFSAP TAIRAIHRDD ADGKLASKCD LSSLRSIWLG
     GERLDSSTFN FLRNITNNKP ILDNYWNTES GSPLITNPSC QVPIKANATG KPMPGYQFHV
     LSPTSERLGA DKIGEVCIKL PVAPGFTNTL YLNPEGYKNA YLNEYPGYLR TADSGYYDEN
     GYYHIISRVD DIINVSGHRL STGSIEEILV KHPKIVECAV IGVHDELKGE IPFGLVVLKP
     QYKDCAEEVE NELIKEVREN IGPVATFKKV LSVNRLPKTR SGKILRNILR KMYNKEEYTV
     PPTIEDMEVL KEIDIEFEKY KLSHPPKK