ACSF_BRASB
ID ACSF_BRASB Reviewed; 353 AA.
AC A5EQ73;
DT 05-FEB-2008, integrated into UniProtKB/Swiss-Prot.
DT 12-JUN-2007, sequence version 1.
DT 25-MAY-2022, entry version 70.
DE RecName: Full=Aerobic magnesium-protoporphyrin IX monomethyl ester [oxidative] cyclase {ECO:0000255|HAMAP-Rule:MF_01840};
DE Short=Aerobic Mg-protoporphyrin IX monomethyl ester oxidative cyclase {ECO:0000255|HAMAP-Rule:MF_01840};
DE EC=1.14.13.81 {ECO:0000255|HAMAP-Rule:MF_01840};
GN Name=acsF {ECO:0000255|HAMAP-Rule:MF_01840}; OrderedLocusNames=BBta_6407;
OS Bradyrhizobium sp. (strain BTAi1 / ATCC BAA-1182).
OC Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales;
OC Bradyrhizobiaceae; Bradyrhizobium; unclassified Bradyrhizobium.
OX NCBI_TaxID=288000;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=BTAi1 / ATCC BAA-1182;
RX PubMed=17540897; DOI=10.1126/science.1139548;
RA Giraud E., Moulin L., Vallenet D., Barbe V., Cytryn E., Avarre J.-C.,
RA Jaubert M., Simon D., Cartieaux F., Prin Y., Bena G., Hannibal L.,
RA Fardoux J., Kojadinovic M., Vuillet L., Lajus A., Cruveiller S., Rouy Z.,
RA Mangenot S., Segurens B., Dossat C., Franck W.L., Chang W.-S., Saunders E.,
RA Bruce D., Richardson P., Normand P., Dreyfus B., Pignol D., Stacey G.,
RA Emerich D., Vermeglio A., Medigue C., Sadowsky M.;
RT "Legumes symbioses: absence of nod genes in photosynthetic bradyrhizobia.";
RL Science 316:1307-1312(2007).
CC -!- FUNCTION: Catalyzes the formation of the isocyclic ring in chlorophyll
CC biosynthesis. Mediates the cyclase reaction, which results in the
CC formation of divinylprotochlorophyllide (Pchlide) characteristic of all
CC chlorophylls from magnesium-protoporphyrin IX 13-monomethyl ester
CC (MgPMME). {ECO:0000255|HAMAP-Rule:MF_01840}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=2 H(+) + Mg-protoporphyrin IX 13-monomethyl ester + 3 NADPH +
CC 3 O2 = 3,8-divinyl protochlorophyllide a + 5 H2O + 3 NADP(+);
CC Xref=Rhea:RHEA:33235, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378,
CC ChEBI:CHEBI:15379, ChEBI:CHEBI:57783, ChEBI:CHEBI:58349,
CC ChEBI:CHEBI:58632, ChEBI:CHEBI:60491; EC=1.14.13.81;
CC Evidence={ECO:0000255|HAMAP-Rule:MF_01840};
CC -!- COFACTOR:
CC Name=Fe cation; Xref=ChEBI:CHEBI:24875;
CC Evidence={ECO:0000255|HAMAP-Rule:MF_01840};
CC -!- PATHWAY: Porphyrin-containing compound metabolism; bacteriochlorophyll
CC biosynthesis (light-independent). {ECO:0000255|HAMAP-Rule:MF_01840}.
CC -!- SIMILARITY: Belongs to the AcsF family. {ECO:0000255|HAMAP-
CC Rule:MF_01840}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; CP000494; ABQ38317.1; -; Genomic_DNA.
DR RefSeq; WP_012046258.1; NC_009485.1.
DR AlphaFoldDB; A5EQ73; -.
DR STRING; 288000.BBta_6407; -.
DR EnsemblBacteria; ABQ38317; ABQ38317; BBta_6407.
DR KEGG; bbt:BBta_6407; -.
DR eggNOG; COG1633; Bacteria.
DR HOGENOM; CLU_048037_0_0_5; -.
DR OMA; FHPIFKW; -.
DR OrthoDB; 366541at2; -.
DR UniPathway; UPA00671; -.
DR Proteomes; UP000000246; Chromosome.
DR GO; GO:0005506; F:iron ion binding; IEA:UniProtKB-UniRule.
DR GO; GO:0048529; F:magnesium-protoporphyrin IX monomethyl ester (oxidative) cyclase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0036070; P:light-independent bacteriochlorophyll biosynthetic process; IEA:UniProtKB-UniPathway.
DR GO; GO:0015979; P:photosynthesis; IEA:UniProtKB-UniRule.
DR CDD; cd01047; ACSF; 1.
DR HAMAP; MF_01840; AcsF; 1.
DR InterPro; IPR008434; AcsF.
DR InterPro; IPR009078; Ferritin-like_SF.
DR InterPro; IPR003251; Rubrerythrin.
DR PANTHER; PTHR31053; PTHR31053; 1.
DR Pfam; PF02915; Rubrerythrin; 1.
DR SUPFAM; SSF47240; SSF47240; 1.
DR TIGRFAMs; TIGR02029; AcsF; 1.
PE 3: Inferred from homology;
KW Bacteriochlorophyll biosynthesis; Chlorophyll biosynthesis; Iron;
KW Metal-binding; NADP; Oxidoreductase; Photosynthesis.
FT CHAIN 1..353
FT /note="Aerobic magnesium-protoporphyrin IX monomethyl ester
FT [oxidative] cyclase"
FT /id="PRO_1000070543"
SQ SEQUENCE 353 AA; 41246 MW; EFDCC63CEEA9326B CRC64;
MIAMEGGAGN ISTKMALEDT ILTPRFYTTD FAAMDRLNVD LVRREWDAVM AELRADHNRK
HFVRTPEFEK DLNELPEALR LEFKDFLVSS LTAEFSGCVL YAEIKKRITN PDIRELFSYM
SRDEARHAGF INEILKDHGI GVDLGFLTKV KKYTYFKPKF IFYATYLSEK IGYARYITIY
RQMERHPERQ FHPIFKWFER WCNDEFRHGE AFALLMRADP KLLSGLNKLW IKFFLLAVFA
TMHVRDHMRP AFYEALGMPV DEYDMRVFRI TSEISRQVFP VMLDIDNPRF WAGLNQLRKV
SEAIADAKAQ GGVMGAIKRA VLPLKAALTF GRLYMLPAKS NELPREIRLQ PAW