ACSF_CYAM1
ID ACSF_CYAM1 Reviewed; 335 AA.
AC Q85FX6;
DT 16-AUG-2005, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-2003, sequence version 1.
DT 03-AUG-2022, entry version 104.
DE RecName: Full=Magnesium-protoporphyrin IX monomethyl ester [oxidative] cyclase {ECO:0000255|HAMAP-Rule:MF_01840};
DE Short=Mg-protoporphyrin IX monomethyl ester oxidative cyclase {ECO:0000255|HAMAP-Rule:MF_01840};
DE EC=1.14.13.81 {ECO:0000255|HAMAP-Rule:MF_01840};
GN Name=acsF {ECO:0000255|HAMAP-Rule:MF_01840}; Synonyms=ycf59;
OS Cyanidioschyzon merolae (strain NIES-3377 / 10D) (Unicellular red alga).
OG Plastid; Chloroplast.
OC Eukaryota; Rhodophyta; Bangiophyceae; Cyanidiales; Cyanidiaceae;
OC Cyanidioschyzon.
OX NCBI_TaxID=280699;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=NIES-3377 / 10D;
RX PubMed=12755171; DOI=10.1093/dnares/10.2.67;
RA Ohta N., Matsuzaki M., Misumi O., Miyagishima S.-Y., Nozaki H., Tanaka K.,
RA Shin-i T., Kohara Y., Kuroiwa T.;
RT "Complete sequence and analysis of the plastid genome of the unicellular
RT red alga Cyanidioschyzon merolae.";
RL DNA Res. 10:67-77(2003).
CC -!- FUNCTION: Catalyzes the formation of the isocyclic ring in chlorophyll
CC biosynthesis. Mediates the cyclase reaction, which results in the
CC formation of divinylprotochlorophyllide (Pchlide) characteristic of all
CC chlorophylls from magnesium-protoporphyrin IX 13-monomethyl ester
CC (MgPMME). {ECO:0000255|HAMAP-Rule:MF_01840}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=2 H(+) + Mg-protoporphyrin IX 13-monomethyl ester + 3 NADPH +
CC 3 O2 = 3,8-divinyl protochlorophyllide a + 5 H2O + 3 NADP(+);
CC Xref=Rhea:RHEA:33235, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378,
CC ChEBI:CHEBI:15379, ChEBI:CHEBI:57783, ChEBI:CHEBI:58349,
CC ChEBI:CHEBI:58632, ChEBI:CHEBI:60491; EC=1.14.13.81;
CC Evidence={ECO:0000255|HAMAP-Rule:MF_01840};
CC -!- COFACTOR:
CC Name=Fe cation; Xref=ChEBI:CHEBI:24875;
CC Evidence={ECO:0000255|HAMAP-Rule:MF_01840};
CC -!- PATHWAY: Porphyrin-containing compound metabolism; chlorophyll
CC biosynthesis (light-independent). {ECO:0000255|HAMAP-Rule:MF_01840}.
CC -!- SUBCELLULAR LOCATION: Plastid, chloroplast.
CC -!- SIMILARITY: Belongs to the AcsF family. {ECO:0000255|HAMAP-
CC Rule:MF_01840}.
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DR EMBL; AB002583; BAC76217.1; -; Genomic_DNA.
DR RefSeq; NP_849055.1; NC_004799.1.
DR AlphaFoldDB; Q85FX6; -.
DR SMR; Q85FX6; -.
DR STRING; 45157.CMV149CT; -.
DR PRIDE; Q85FX6; -.
DR EnsemblPlants; CMV149CT; CMV149CT; CMV149C.
DR GeneID; 845087; -.
DR Gramene; CMV149CT; CMV149CT; CMV149C.
DR KEGG; cme:CymeCp123; -.
DR eggNOG; ENOG502QRIH; Eukaryota.
DR HOGENOM; CLU_048037_0_0_1; -.
DR UniPathway; UPA00670; -.
DR Proteomes; UP000007014; Chloroplast.
DR GO; GO:0009706; C:chloroplast inner membrane; IEA:EnsemblPlants.
DR GO; GO:0009535; C:chloroplast thylakoid membrane; IEA:EnsemblPlants.
DR GO; GO:0005506; F:iron ion binding; IEA:UniProtKB-UniRule.
DR GO; GO:0048529; F:magnesium-protoporphyrin IX monomethyl ester (oxidative) cyclase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0003729; F:mRNA binding; IEA:EnsemblPlants.
DR GO; GO:0009658; P:chloroplast organization; IEA:EnsemblPlants.
DR GO; GO:0036068; P:light-independent chlorophyll biosynthetic process; IEA:UniProtKB-UniPathway.
DR GO; GO:0015979; P:photosynthesis; IEA:UniProtKB-UniRule.
DR GO; GO:1901401; P:regulation of tetrapyrrole metabolic process; IEA:EnsemblPlants.
DR CDD; cd01047; ACSF; 1.
DR HAMAP; MF_01840; AcsF; 1.
DR InterPro; IPR008434; AcsF.
DR InterPro; IPR009078; Ferritin-like_SF.
DR InterPro; IPR003251; Rubrerythrin.
DR PANTHER; PTHR31053; PTHR31053; 1.
DR Pfam; PF02915; Rubrerythrin; 1.
DR SUPFAM; SSF47240; SSF47240; 1.
DR TIGRFAMs; TIGR02029; AcsF; 1.
PE 3: Inferred from homology;
KW Chlorophyll biosynthesis; Chloroplast; Iron; Metal-binding; NADP;
KW Oxidoreductase; Photosynthesis; Plastid; Reference proteome.
FT CHAIN 1..335
FT /note="Magnesium-protoporphyrin IX monomethyl ester
FT [oxidative] cyclase"
FT /id="PRO_0000217542"
SQ SEQUENCE 335 AA; 40024 MW; BE3A1610AABF2915 CRC64;
MQTAVQTQTT VQTLLTPRFY TTDFDQMAKY DISKNRAEIE AIVNEFRKDY NQTHFIRDDE
FDQVWTQMPE SKRQWFKEFL ERSCTAEFSG FLLYKELSRR LKDTNPLLAE GFGYMSRDEA
RHAGFLNKAM ADFGVSLDLG FLTKHRQYTF FAPKFIFYAT YLSEKIGYWR YITIYRHLEA
HPKWSVYPIF KFFEKWCQDE NRHGDFFAAI LKAQPKWITG WASRLWCRFF LLSVFVTMYL
NDLQRKDFYQ LIGLDARAFD WHVIRKTNES AARLFPVVLD VDHVDFVRLM DECACAYAHW
RETQSVLSLA KIFACLFKLY VIKPINTQAL WNTIK
