ID   CYSK2_ORYSJ             Reviewed;         325 AA.
AC   Q9XEA8; A0A0P0W3J8; Q10CX6; Q7G7J5; Q8W313;
DT   01-DEC-2000, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1999, sequence version 1.
DT   03-AUG-2022, entry version 142.
DE   RecName: Full=Cysteine synthase;
DE            Short=CSase;
DE            EC=2.5.1.47;
DE   AltName: Full=O-acetylserine (thiol)-lyase;
DE            Short=OAS-TL;
DE   AltName: Full=O-acetylserine sulfhydrylase;
GN   Name=RCS3;
GN   OrderedLocusNames=Os03g0747800 {ECO:0000312|EMBL:BAF13176.1},
GN   LOC_Os03g53650 {ECO:0000312|EMBL:ABF98862.1};
GN   ORFNames=OJ1124_H03.2, OSJNBa0069E14.1;
OS   Oryza sativa subsp. japonica (Rice).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC   Oryzoideae; Oryzeae; Oryzinae; Oryza; Oryza sativa.
OX   NCBI_TaxID=39947;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RC   STRAIN=cv. Nipponbare;
RX   PubMed=10095115; DOI=10.1016/s0378-1119(99)00019-0;
RA   Nakamura T., Yamaguchi Y., Sano H.;
RT   "Four rice genes encoding cysteine synthase: isolation and differential
RT   responses to sulfur, nitrogen and light.";
RL   Gene 229:155-161(1999).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Nipponbare;
RX   PubMed=16109971; DOI=10.1101/gr.3869505;
RG   The rice chromosome 3 sequencing consortium;
RA   Buell C.R., Yuan Q., Ouyang S., Liu J., Zhu W., Wang A., Maiti R., Haas B.,
RA   Wortman J., Pertea M., Jones K.M., Kim M., Overton L., Tsitrin T.,
RA   Fadrosh D., Bera J., Weaver B., Jin S., Johri S., Reardon M., Webb K.,
RA   Hill J., Moffat K., Tallon L., Van Aken S., Lewis M., Utterback T.,
RA   Feldblyum T., Zismann V., Iobst S., Hsiao J., de Vazeille A.R.,
RA   Salzberg S.L., White O., Fraser C.M., Yu Y., Kim H., Rambo T., Currie J.,
RA   Collura K., Kernodle-Thompson S., Wei F., Kudrna K., Ammiraju J.S.S.,
RA   Luo M., Goicoechea J.L., Wing R.A., Henry D., Oates R., Palmer M.,
RA   Pries G., Saski C., Simmons J., Soderlund C., Nelson W., de la Bastide M.,
RA   Spiegel L., Nascimento L., Huang E., Preston R., Zutavern T., Palmer L.,
RA   O'Shaughnessy A., Dike S., McCombie W.R., Minx P., Cordum H., Wilson R.,
RA   Jin W., Lee H.R., Jiang J., Jackson S.;
RT   "Sequence, annotation, and analysis of synteny between rice chromosome 3
RT   and diverged grass species.";
RL   Genome Res. 15:1284-1291(2005).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Nipponbare;
RX   PubMed=16100779; DOI=10.1038/nature03895;
RG   International rice genome sequencing project (IRGSP);
RT   "The map-based sequence of the rice genome.";
RL   Nature 436:793-800(2005).
RN   [4]
RP   GENOME REANNOTATION.
RC   STRAIN=cv. Nipponbare;
RX   PubMed=18089549; DOI=10.1093/nar/gkm978;
RG   The rice annotation project (RAP);
RT   "The rice annotation project database (RAP-DB): 2008 update.";
RL   Nucleic Acids Res. 36:D1028-D1033(2008).
RN   [5]
RP   GENOME REANNOTATION.
RC   STRAIN=cv. Nipponbare;
RX   PubMed=24280374; DOI=10.1186/1939-8433-6-4;
RA   Kawahara Y., de la Bastide M., Hamilton J.P., Kanamori H., McCombie W.R.,
RA   Ouyang S., Schwartz D.C., Tanaka T., Wu J., Zhou S., Childs K.L.,
RA   Davidson R.M., Lin H., Quesada-Ocampo L., Vaillancourt B., Sakai H.,
RA   Lee S.S., Kim J., Numa H., Itoh T., Buell C.R., Matsumoto T.;
RT   "Improvement of the Oryza sativa Nipponbare reference genome using next
RT   generation sequence and optical map data.";
RL   Rice 6:4-4(2013).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=hydrogen sulfide + O-acetyl-L-serine = acetate + L-cysteine;
CC         Xref=Rhea:RHEA:14829, ChEBI:CHEBI:29919, ChEBI:CHEBI:30089,
CC         ChEBI:CHEBI:35235, ChEBI:CHEBI:58340; EC=2.5.1.47;
CC   -!- COFACTOR:
CC       Name=pyridoxal 5'-phosphate; Xref=ChEBI:CHEBI:597326;
CC         Evidence={ECO:0000250};
CC   -!- PATHWAY: Amino-acid biosynthesis; L-cysteine biosynthesis; L-cysteine
CC       from L-serine: step 2/2.
CC   -!- SUBUNIT: Homodimer. {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the cysteine synthase/cystathionine beta-
CC       synthase family. {ECO:0000305}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=AAL58961.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR   EMBL; AF073697; AAD23909.1; -; mRNA.
DR   EMBL; AC087852; AAK71541.1; -; Genomic_DNA.
DR   EMBL; AC091811; AAL58961.1; ALT_SEQ; Genomic_DNA.
DR   EMBL; DP000009; ABF98862.1; -; Genomic_DNA.
DR   EMBL; DP000009; ABF98864.1; -; Genomic_DNA.
DR   EMBL; AP008209; BAF13176.1; -; Genomic_DNA.
DR   EMBL; AP014959; BAS86372.1; -; Genomic_DNA.
DR   RefSeq; XP_015633055.1; XM_015777569.1.
DR   RefSeq; XP_015633056.1; XM_015777570.1.
DR   AlphaFoldDB; Q9XEA8; -.
DR   SMR; Q9XEA8; -.
DR   STRING; 4530.OS03T0747800-01; -.
DR   PaxDb; Q9XEA8; -.
DR   PRIDE; Q9XEA8; -.
DR   EnsemblPlants; Os03t0747800-01; Os03t0747800-01; Os03g0747800.
DR   GeneID; 4334101; -.
DR   Gramene; Os03t0747800-01; Os03t0747800-01; Os03g0747800.
DR   KEGG; osa:4334101; -.
DR   eggNOG; KOG1252; Eukaryota.
DR   HOGENOM; CLU_021018_1_0_1; -.
DR   InParanoid; Q9XEA8; -.
DR   OMA; DYIMLDQ; -.
DR   OrthoDB; 1016546at2759; -.
DR   BRENDA; 2.5.1.47; 8948.
DR   PlantReactome; R-OSA-1119331; Cysteine biosynthesis I.
DR   UniPathway; UPA00136; UER00200.
DR   Proteomes; UP000000763; Chromosome 3.
DR   Proteomes; UP000059680; Chromosome 3.
DR   Genevisible; Q9XEA8; OS.
DR   GO; GO:0005737; C:cytoplasm; IBA:GO_Central.
DR   GO; GO:0004124; F:cysteine synthase activity; IBA:GO_Central.
DR   GO; GO:0019344; P:cysteine biosynthetic process; IBA:GO_Central.
DR   GO; GO:0006535; P:cysteine biosynthetic process from serine; IBA:GO_Central.
DR   Gene3D; 3.40.50.1100; -; 2.
DR   InterPro; IPR005856; Cys_synth.
DR   InterPro; IPR005859; CysK.
DR   InterPro; IPR001216; P-phosphate_BS.
DR   InterPro; IPR001926; PLP-dep.
DR   InterPro; IPR036052; Trypto_synt_PLP_dependent.
DR   Pfam; PF00291; PALP; 1.
DR   SUPFAM; SSF53686; SSF53686; 1.
DR   TIGRFAMs; TIGR01139; cysK; 1.
DR   TIGRFAMs; TIGR01136; cysKM; 1.
DR   PROSITE; PS00901; CYS_SYNTHASE; 1.
PE   2: Evidence at transcript level;
KW   Amino-acid biosynthesis; Cysteine biosynthesis; Cytoplasm;
KW   Pyridoxal phosphate; Reference proteome; Transferase.
FT   CHAIN           1..325
FT                   /note="Cysteine synthase"
FT                   /id="PRO_0000167122"
FT   BINDING         80
FT                   /ligand="pyridoxal 5'-phosphate"
FT                   /ligand_id="ChEBI:CHEBI:597326"
FT                   /evidence="ECO:0000250"
FT   BINDING         184..188
FT                   /ligand="pyridoxal 5'-phosphate"
FT                   /ligand_id="ChEBI:CHEBI:597326"
FT                   /evidence="ECO:0000250"
FT   BINDING         272
FT                   /ligand="pyridoxal 5'-phosphate"
FT                   /ligand_id="ChEBI:CHEBI:597326"
FT                   /evidence="ECO:0000250"
FT   MOD_RES         49
FT                   /note="N6-(pyridoxal phosphate)lysine"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   325 AA;  34307 MW;  8F6B0CDCCD7DCAC4 CRC64;
     MAESGQSIAS DVTALIGNTP LVYLNKVVDG CEAQIAAKLE IMEPCSSVKD RIGYSMITDA
     EEKGLITPGK SVLIEPTSGN TGIGLAFMAA AKGYKLILTM PASMSMERRI ILKAFGAELV
     LTDPLLGMKG AIQKADELAA KMPNSYILQQ FENPANPKIH YETTGPEIWK ATAGKVDILV
     SGIGTGGTVT GTGKYLKEQN PEIKIYGVEP TESAILSGGR PGPHKIQGIG AGFVPGVLDV
     NLLDEVVQVS SDEAISMAKQ LALKEGLLVG ISSGAAAVAA IRVAQRPENK GKLVVVVFPS
     FGERYLSSVL FESIKREAEN MVFEP