CYSK_SOLTU
ID CYSK_SOLTU Reviewed; 325 AA.
AC O81154;
DT 01-DEC-2000, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1998, sequence version 1.
DT 03-AUG-2022, entry version 106.
DE RecName: Full=Cysteine synthase;
DE EC=2.5.1.47;
DE AltName: Full=CSase A;
DE Short=CS-A;
DE AltName: Full=O-acetylserine (thiol)-lyase;
DE Short=OAS-TL A;
DE AltName: Full=O-acetylserine sulfhydrylase;
OS Solanum tuberosum (Potato).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC asterids; lamiids; Solanales; Solanaceae; Solanoideae; Solaneae; Solanum.
OX NCBI_TaxID=4113;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC STRAIN=cv. Berolina;
RA Hesse H., Hoefgen R.;
RT "Isolation of cDNAs encoding cytosolic and plastidic cysteine synthase
RT isoforms from Solanum tuberosum.";
RL (er) Plant Gene Register PGR98-057(1998).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=hydrogen sulfide + O-acetyl-L-serine = acetate + L-cysteine;
CC Xref=Rhea:RHEA:14829, ChEBI:CHEBI:29919, ChEBI:CHEBI:30089,
CC ChEBI:CHEBI:35235, ChEBI:CHEBI:58340; EC=2.5.1.47;
CC -!- COFACTOR:
CC Name=pyridoxal 5'-phosphate; Xref=ChEBI:CHEBI:597326;
CC -!- PATHWAY: Amino-acid biosynthesis; L-cysteine biosynthesis; L-cysteine
CC from L-serine: step 2/2.
CC -!- SUBUNIT: Homodimer. {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the cysteine synthase/cystathionine beta-
CC synthase family. {ECO:0000305}.
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DR EMBL; AF044172; AAC25635.1; -; mRNA.
DR PIR; T07001; T07001.
DR AlphaFoldDB; O81154; -.
DR SMR; O81154; -.
DR STRING; 4113.PGSC0003DMT400082113; -.
DR PRIDE; O81154; -.
DR eggNOG; KOG1252; Eukaryota.
DR BRENDA; 2.5.1.47; 5757.
DR UniPathway; UPA00136; UER00200.
DR Proteomes; UP000011115; Unassembled WGS sequence.
DR ExpressionAtlas; O81154; baseline.
DR GO; GO:0005737; C:cytoplasm; IBA:GO_Central.
DR GO; GO:0004124; F:cysteine synthase activity; IBA:GO_Central.
DR GO; GO:0019344; P:cysteine biosynthetic process; IBA:GO_Central.
DR GO; GO:0006535; P:cysteine biosynthetic process from serine; IBA:GO_Central.
DR Gene3D; 3.40.50.1100; -; 2.
DR InterPro; IPR005856; Cys_synth.
DR InterPro; IPR005859; CysK.
DR InterPro; IPR001216; P-phosphate_BS.
DR InterPro; IPR001926; PLP-dep.
DR InterPro; IPR036052; Trypto_synt_PLP_dependent.
DR Pfam; PF00291; PALP; 1.
DR SUPFAM; SSF53686; SSF53686; 1.
DR TIGRFAMs; TIGR01139; cysK; 1.
DR TIGRFAMs; TIGR01136; cysKM; 1.
DR PROSITE; PS00901; CYS_SYNTHASE; 1.
PE 2: Evidence at transcript level;
KW Amino-acid biosynthesis; Cysteine biosynthesis; Cytoplasm;
KW Pyridoxal phosphate; Reference proteome; Transferase.
FT CHAIN 1..325
FT /note="Cysteine synthase"
FT /id="PRO_0000167124"
FT BINDING 80
FT /ligand="pyridoxal 5'-phosphate"
FT /ligand_id="ChEBI:CHEBI:597326"
FT /evidence="ECO:0000250"
FT BINDING 184..188
FT /ligand="pyridoxal 5'-phosphate"
FT /ligand_id="ChEBI:CHEBI:597326"
FT /evidence="ECO:0000250"
FT BINDING 272
FT /ligand="pyridoxal 5'-phosphate"
FT /ligand_id="ChEBI:CHEBI:597326"
FT /evidence="ECO:0000250"
FT MOD_RES 49
FT /note="N6-(pyridoxal phosphate)lysine"
FT /evidence="ECO:0000250"
SQ SEQUENCE 325 AA; 34342 MW; 86119D0466C647BC CRC64;
MAGEKIGIAK DVTELIGNTP LVYLNNVVDG CVARVAAKLE SMEPCSSVKD RIGYSMITDA
EEKGLIKPGE SVLIEPTSGN TGVGLAFMAA AKGYKLIITM PSSMSLERRI ILRGFRSELV
LTDPAKGMKG AISKAEEIKA KTPNSYILQQ FENPANPKIH YETTGPEIWK GSNGKVDALA
SGIGTGGTIT GSGKYLREQN PNVKLYGVEP VESAILSGGK PGPHKIQGIG AGFIPGVLEV
NLIDDVVQVS SEESIEMAKL LALKEGLLVG ISSGAAAAAA IKVAKRPENA GKLIVVIFPS
FGERYLSSVL FETVRREAEN MTVEP
