CYSK_WHEAT
ID CYSK_WHEAT Reviewed; 325 AA.
AC P38076;
DT 01-OCT-1994, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-1994, sequence version 1.
DT 03-AUG-2022, entry version 110.
DE RecName: Full=Cysteine synthase;
DE EC=2.5.1.47;
DE AltName: Full=CSase A;
DE AltName: Full=O-acetylserine (thiol)-lyase;
DE Short=OAS-TL A;
DE AltName: Full=O-acetylserine sulfhydrylase;
GN Name=CYS1;
OS Triticum aestivum (Wheat).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC Pooideae; Triticodae; Triticeae; Triticinae; Triticum.
OX NCBI_TaxID=4565;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC TISSUE=Leaf;
RX PubMed=8275097; DOI=10.1046/j.1365-313x.1993.04050759.x;
RA Youssefian S., Nakamura M., Sano H.;
RT "Tobacco plants transformed with the O-acetylserine (thiol) lyase gene of
RT wheat are resistant to toxic levels of hydrogen sulphide gas.";
RL Plant J. 4:759-769(1993).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=hydrogen sulfide + O-acetyl-L-serine = acetate + L-cysteine;
CC Xref=Rhea:RHEA:14829, ChEBI:CHEBI:29919, ChEBI:CHEBI:30089,
CC ChEBI:CHEBI:35235, ChEBI:CHEBI:58340; EC=2.5.1.47;
CC -!- COFACTOR:
CC Name=pyridoxal 5'-phosphate; Xref=ChEBI:CHEBI:597326;
CC -!- PATHWAY: Amino-acid biosynthesis; L-cysteine biosynthesis; L-cysteine
CC from L-serine: step 2/2.
CC -!- SUBUNIT: Homodimer. {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the cysteine synthase/cystathionine beta-
CC synthase family. {ECO:0000305}.
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DR EMBL; D13153; BAA02438.1; -; mRNA.
DR PIR; JS0762; JS0762.
DR AlphaFoldDB; P38076; -.
DR SMR; P38076; -.
DR STRING; 4565.Traes_5BS_1AC8D3009.2; -.
DR PRIDE; P38076; -.
DR EnsemblPlants; TraesCAD_scaffold_028245_01G000300.1; TraesCAD_scaffold_028245_01G000300.1; TraesCAD_scaffold_028245_01G000300.
DR EnsemblPlants; TraesCLE_scaffold_118677_01G000300.1; TraesCLE_scaffold_118677_01G000300.1; TraesCLE_scaffold_118677_01G000300.
DR EnsemblPlants; TraesPAR_scaffold_031629_01G000200.1; TraesPAR_scaffold_031629_01G000200.1; TraesPAR_scaffold_031629_01G000200.
DR EnsemblPlants; TraesROB_scaffold_078535_01G000300.1; TraesROB_scaffold_078535_01G000300.1; TraesROB_scaffold_078535_01G000300.
DR EnsemblPlants; TraesWEE_scaffold_078045_01G000100.1; TraesWEE_scaffold_078045_01G000100.1; TraesWEE_scaffold_078045_01G000100.
DR Gramene; TraesCAD_scaffold_028245_01G000300.1; TraesCAD_scaffold_028245_01G000300.1; TraesCAD_scaffold_028245_01G000300.
DR Gramene; TraesCLE_scaffold_118677_01G000300.1; TraesCLE_scaffold_118677_01G000300.1; TraesCLE_scaffold_118677_01G000300.
DR Gramene; TraesPAR_scaffold_031629_01G000200.1; TraesPAR_scaffold_031629_01G000200.1; TraesPAR_scaffold_031629_01G000200.
DR Gramene; TraesROB_scaffold_078535_01G000300.1; TraesROB_scaffold_078535_01G000300.1; TraesROB_scaffold_078535_01G000300.
DR Gramene; TraesWEE_scaffold_078045_01G000100.1; TraesWEE_scaffold_078045_01G000100.1; TraesWEE_scaffold_078045_01G000100.
DR eggNOG; KOG1252; Eukaryota.
DR UniPathway; UPA00136; UER00200.
DR Proteomes; UP000019116; Unplaced.
DR ExpressionAtlas; P38076; baseline and differential.
DR GO; GO:0005737; C:cytoplasm; IBA:GO_Central.
DR GO; GO:0004124; F:cysteine synthase activity; IBA:GO_Central.
DR GO; GO:0019344; P:cysteine biosynthetic process; IBA:GO_Central.
DR GO; GO:0006535; P:cysteine biosynthetic process from serine; IBA:GO_Central.
DR Gene3D; 3.40.50.1100; -; 2.
DR InterPro; IPR005856; Cys_synth.
DR InterPro; IPR005859; CysK.
DR InterPro; IPR001216; P-phosphate_BS.
DR InterPro; IPR001926; PLP-dep.
DR InterPro; IPR036052; Trypto_synt_PLP_dependent.
DR Pfam; PF00291; PALP; 1.
DR SUPFAM; SSF53686; SSF53686; 1.
DR TIGRFAMs; TIGR01139; cysK; 1.
DR TIGRFAMs; TIGR01136; cysKM; 1.
DR PROSITE; PS00901; CYS_SYNTHASE; 1.
PE 2: Evidence at transcript level;
KW Amino-acid biosynthesis; Cysteine biosynthesis; Cytoplasm;
KW Pyridoxal phosphate; Reference proteome; Transferase.
FT CHAIN 1..325
FT /note="Cysteine synthase"
FT /id="PRO_0000167126"
FT BINDING 81
FT /ligand="pyridoxal 5'-phosphate"
FT /ligand_id="ChEBI:CHEBI:597326"
FT /evidence="ECO:0000250"
FT BINDING 185..189
FT /ligand="pyridoxal 5'-phosphate"
FT /ligand_id="ChEBI:CHEBI:597326"
FT /evidence="ECO:0000250"
FT BINDING 273
FT /ligand="pyridoxal 5'-phosphate"
FT /ligand_id="ChEBI:CHEBI:597326"
FT /evidence="ECO:0000250"
FT MOD_RES 50
FT /note="N6-(pyridoxal phosphate)lysine"
FT /evidence="ECO:0000250"
SQ SEQUENCE 325 AA; 34114 MW; 1CEF8FE29F341DCA CRC64;
MGEASSPAIA KDVTELIGNT PLVYLNKVTD GCVGRVAAKL ESMEPCSSVK DRIGYSMITD
AEEKGFIVPG KSVLIEPTSG NTGIGLAFMA AAKGYRLVLT MPASMSMERR IILKAFGAEL
ILTDPLLGMK GAVQKAEELA AKTPNSYILQ QFENAANPKI HYETTGPEIW KGTGGKIDGL
VSGIGTGGTI TGTGKYLQEQ NPNIKLYGVE PTESAILNGG KPGPHKIQGI GAGFIPGVLD
VDIIDETIQV SSDESIEMAK SLALKEGLLV GISSGAAAAA AIKVAQRPEN AGKLFVVVFP
SFGERYLSSV LFHSIKKEAE SMVVE
