CYSM_AQUAE
ID CYSM_AQUAE Reviewed; 327 AA.
AC O67507;
DT 30-MAY-2000, integrated into UniProtKB/Swiss-Prot.
DT 01-AUG-1998, sequence version 1.
DT 03-AUG-2022, entry version 123.
DE RecName: Full=Cysteine synthase;
DE Short=CSase;
DE EC=2.5.1.47;
DE AltName: Full=O-acetylserine (thiol)-lyase;
DE Short=OAS-TL;
DE AltName: Full=O-acetylserine sulfhydrylase;
GN Name=cysM; OrderedLocusNames=aq_1556;
OS Aquifex aeolicus (strain VF5).
OC Bacteria; Aquificae; Aquificales; Aquificaceae; Aquifex.
OX NCBI_TaxID=224324;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=VF5;
RX PubMed=9537320; DOI=10.1038/32831;
RA Deckert G., Warren P.V., Gaasterland T., Young W.G., Lenox A.L.,
RA Graham D.E., Overbeek R., Snead M.A., Keller M., Aujay M., Huber R.,
RA Feldman R.A., Short J.M., Olsen G.J., Swanson R.V.;
RT "The complete genome of the hyperthermophilic bacterium Aquifex aeolicus.";
RL Nature 392:353-358(1998).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=hydrogen sulfide + O-acetyl-L-serine = acetate + L-cysteine;
CC Xref=Rhea:RHEA:14829, ChEBI:CHEBI:29919, ChEBI:CHEBI:30089,
CC ChEBI:CHEBI:35235, ChEBI:CHEBI:58340; EC=2.5.1.47;
CC -!- COFACTOR:
CC Name=pyridoxal 5'-phosphate; Xref=ChEBI:CHEBI:597326;
CC -!- PATHWAY: Amino-acid biosynthesis; L-cysteine biosynthesis; L-cysteine
CC from L-serine: step 2/2.
CC -!- SIMILARITY: Belongs to the cysteine synthase/cystathionine beta-
CC synthase family. {ECO:0000305}.
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DR EMBL; AE000657; AAC07459.1; -; Genomic_DNA.
DR PIR; A70435; A70435.
DR RefSeq; NP_214072.1; NC_000918.1.
DR AlphaFoldDB; O67507; -.
DR SMR; O67507; -.
DR STRING; 224324.aq_1556; -.
DR EnsemblBacteria; AAC07459; AAC07459; aq_1556.
DR KEGG; aae:aq_1556; -.
DR PATRIC; fig|224324.8.peg.1206; -.
DR eggNOG; COG0031; Bacteria.
DR HOGENOM; CLU_021018_1_0_0; -.
DR InParanoid; O67507; -.
DR OMA; CNGGFFA; -.
DR OrthoDB; 1033353at2; -.
DR UniPathway; UPA00136; UER00200.
DR Proteomes; UP000000798; Chromosome.
DR GO; GO:0005737; C:cytoplasm; IBA:GO_Central.
DR GO; GO:0004124; F:cysteine synthase activity; IEA:UniProtKB-EC.
DR GO; GO:0019344; P:cysteine biosynthetic process; IBA:GO_Central.
DR GO; GO:0006535; P:cysteine biosynthetic process from serine; IEA:InterPro.
DR Gene3D; 3.40.50.1100; -; 2.
DR InterPro; IPR005856; Cys_synth.
DR InterPro; IPR005858; CysM.
DR InterPro; IPR001216; P-phosphate_BS.
DR InterPro; IPR001926; PLP-dep.
DR InterPro; IPR036052; Trypto_synt_PLP_dependent.
DR Pfam; PF00291; PALP; 1.
DR SUPFAM; SSF53686; SSF53686; 1.
DR TIGRFAMs; TIGR01136; cysKM; 1.
DR TIGRFAMs; TIGR01138; cysM; 1.
DR PROSITE; PS00901; CYS_SYNTHASE; 1.
PE 3: Inferred from homology;
KW Amino-acid biosynthesis; Cysteine biosynthesis; Pyridoxal phosphate;
KW Reference proteome; Transferase.
FT CHAIN 1..327
FT /note="Cysteine synthase"
FT /id="PRO_0000167106"
FT BINDING 95
FT /ligand="pyridoxal 5'-phosphate"
FT /ligand_id="ChEBI:CHEBI:597326"
FT /evidence="ECO:0000250"
FT BINDING 200..204
FT /ligand="pyridoxal 5'-phosphate"
FT /ligand_id="ChEBI:CHEBI:597326"
FT /evidence="ECO:0000250"
FT BINDING 282
FT /ligand="pyridoxal 5'-phosphate"
FT /ligand_id="ChEBI:CHEBI:597326"
FT /evidence="ECO:0000250"
FT MOD_RES 65
FT /note="N6-(pyridoxal phosphate)lysine"
FT /evidence="ECO:0000250"
SQ SEQUENCE 327 AA; 36155 MW; 54FC4338949FB40E CRC64;
MSLKSCLMWV LGEHDEPYRK ARETILKLVG NTPLVRVKKC LPEDIPEDVE IWAKLESFNP
GGSVKDRPAL SMFLDALKRG LIKEGKVVID ATSGNTGIAL AMVGAALGVP VELAMPANVS
EERKKIIKAF GAKLYLTDPL EGTDGAILFV RELVQKYPEK YVYLDQYNNP ANWKAHFYST
GIEIWNQTKG RITHFVAGIG TGGTIMGTGR RLKIYNPNIQ VIGVQPAYPF HGIEGLKHIE
SSIKPGIFDE TFLDRTIFVE TEDAYYWARE LAKKDAIFVG QSSGAALWAC IQLARELAKK
GEKGVIVTVF PDGGEKYLTT ALFSDKD
