CYSM_PSEAE
ID CYSM_PSEAE Reviewed; 299 AA.
AC Q9I526;
DT 15-MAY-2007, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2001, sequence version 1.
DT 03-AUG-2022, entry version 120.
DE RecName: Full=Cysteine synthase B;
DE Short=CSase B;
DE EC=2.5.1.47;
DE AltName: Full=O-acetylserine (thiol)-lyase B;
DE Short=OAS-TL B;
DE AltName: Full=O-acetylserine sulfhydrylase B;
GN Name=cysM; OrderedLocusNames=PA0932;
OS Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM
OS 14847 / LMG 12228 / 1C / PRS 101 / PAO1).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales;
OC Pseudomonadaceae; Pseudomonas.
OX NCBI_TaxID=208964;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C /
RC PRS 101 / PAO1;
RX PubMed=10984043; DOI=10.1038/35023079;
RA Stover C.K., Pham X.-Q.T., Erwin A.L., Mizoguchi S.D., Warrener P.,
RA Hickey M.J., Brinkman F.S.L., Hufnagle W.O., Kowalik D.J., Lagrou M.,
RA Garber R.L., Goltry L., Tolentino E., Westbrock-Wadman S., Yuan Y.,
RA Brody L.L., Coulter S.N., Folger K.R., Kas A., Larbig K., Lim R.M.,
RA Smith K.A., Spencer D.H., Wong G.K.-S., Wu Z., Paulsen I.T., Reizer J.,
RA Saier M.H. Jr., Hancock R.E.W., Lory S., Olson M.V.;
RT "Complete genome sequence of Pseudomonas aeruginosa PAO1, an opportunistic
RT pathogen.";
RL Nature 406:959-964(2000).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=hydrogen sulfide + O-acetyl-L-serine = acetate + L-cysteine;
CC Xref=Rhea:RHEA:14829, ChEBI:CHEBI:29919, ChEBI:CHEBI:30089,
CC ChEBI:CHEBI:35235, ChEBI:CHEBI:58340; EC=2.5.1.47;
CC -!- COFACTOR:
CC Name=pyridoxal 5'-phosphate; Xref=ChEBI:CHEBI:597326;
CC Evidence={ECO:0000250};
CC -!- PATHWAY: Amino-acid biosynthesis; L-cysteine biosynthesis; L-cysteine
CC from L-serine: step 2/2.
CC -!- SIMILARITY: Belongs to the cysteine synthase/cystathionine beta-
CC synthase family. {ECO:0000305}.
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DR EMBL; AE004091; AAG04321.1; -; Genomic_DNA.
DR PIR; A83530; A83530.
DR RefSeq; NP_249623.1; NC_002516.2.
DR RefSeq; WP_003102419.1; NZ_QZGE01000007.1.
DR AlphaFoldDB; Q9I526; -.
DR SMR; Q9I526; -.
DR STRING; 287.DR97_1007; -.
DR PaxDb; Q9I526; -.
DR PRIDE; Q9I526; -.
DR EnsemblBacteria; AAG04321; AAG04321; PA0932.
DR GeneID; 881832; -.
DR KEGG; pae:PA0932; -.
DR PATRIC; fig|208964.12.peg.967; -.
DR PseudoCAP; PA0932; -.
DR HOGENOM; CLU_021018_1_0_6; -.
DR InParanoid; Q9I526; -.
DR OMA; GIRRWPE; -.
DR PhylomeDB; Q9I526; -.
DR BioCyc; PAER208964:G1FZ6-952-MON; -.
DR UniPathway; UPA00136; UER00200.
DR Proteomes; UP000002438; Chromosome.
DR GO; GO:0005737; C:cytoplasm; IBA:GO_Central.
DR GO; GO:0004124; F:cysteine synthase activity; IBA:GO_Central.
DR GO; GO:0080146; F:L-cysteine desulfhydrase activity; IBA:GO_Central.
DR GO; GO:0019344; P:cysteine biosynthetic process; IBA:GO_Central.
DR GO; GO:0006535; P:cysteine biosynthetic process from serine; IEA:InterPro.
DR Gene3D; 3.40.50.1100; -; 2.
DR InterPro; IPR005856; Cys_synth.
DR InterPro; IPR005858; CysM.
DR InterPro; IPR001216; P-phosphate_BS.
DR InterPro; IPR001926; PLP-dep.
DR InterPro; IPR036052; Trypto_synt_PLP_dependent.
DR PANTHER; PTHR10314:SF100; PTHR10314:SF100; 1.
DR Pfam; PF00291; PALP; 1.
DR SUPFAM; SSF53686; SSF53686; 1.
DR TIGRFAMs; TIGR01136; cysKM; 1.
DR TIGRFAMs; TIGR01138; cysM; 1.
DR PROSITE; PS00901; CYS_SYNTHASE; 1.
PE 3: Inferred from homology;
KW Amino-acid biosynthesis; Cysteine biosynthesis; Pyridoxal phosphate;
KW Reference proteome; Transferase.
FT CHAIN 1..299
FT /note="Cysteine synthase B"
FT /id="PRO_0000287759"
FT BINDING 75
FT /ligand="pyridoxal 5'-phosphate"
FT /ligand_id="ChEBI:CHEBI:597326"
FT /evidence="ECO:0000250"
FT BINDING 178..182
FT /ligand="pyridoxal 5'-phosphate"
FT /ligand_id="ChEBI:CHEBI:597326"
FT /evidence="ECO:0000250"
FT BINDING 259
FT /ligand="pyridoxal 5'-phosphate"
FT /ligand_id="ChEBI:CHEBI:597326"
FT /evidence="ECO:0000250"
FT MOD_RES 45
FT /note="N6-(pyridoxal phosphate)lysine"
FT /evidence="ECO:0000250"
SQ SEQUENCE 299 AA; 32448 MW; 9B78F6986CD14CC8 CRC64;
MTVQYPTIAD CVGNTPLVRL QRLPGETSNT LLVKLEGNNP AGSVKDRPAL SMITRAELRG
DIRPGDTLIE ATSGNTGIAL AMAAAIKGYK MILIMPDNST AERKAAMTAY GAELILVSKE
EGMEGARDLA DKLQREGRGK VLDQFANGDN PEAHYHSTGP EIWQQTGGSI THFVSSMGTT
GTIMGVSRYL KEQNPAVQIV GLQPMEGSAI PGIRRWPQEY LPKIYDASRV DRVVDMHQDE
AEDIMRRLAR EEGIFCGVSS GGAVAAMLRL SRELENAVLV AIICDRGDRY LSSGVYDPR
