ACSF_RHOPA
ID ACSF_RHOPA Reviewed; 365 AA.
AC Q6N9J7;
DT 16-AUG-2005, integrated into UniProtKB/Swiss-Prot.
DT 05-JUL-2004, sequence version 1.
DT 03-AUG-2022, entry version 102.
DE RecName: Full=Aerobic magnesium-protoporphyrin IX monomethyl ester [oxidative] cyclase {ECO:0000255|HAMAP-Rule:MF_01840};
DE Short=Aerobic Mg-protoporphyrin IX monomethyl ester oxidative cyclase {ECO:0000255|HAMAP-Rule:MF_01840};
DE EC=1.14.13.81 {ECO:0000255|HAMAP-Rule:MF_01840};
GN Name=acsF {ECO:0000255|HAMAP-Rule:MF_01840}; OrderedLocusNames=RPA1552;
OS Rhodopseudomonas palustris (strain ATCC BAA-98 / CGA009).
OC Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales;
OC Bradyrhizobiaceae; Rhodopseudomonas.
OX NCBI_TaxID=258594;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC BAA-98 / CGA009;
RX PubMed=14704707; DOI=10.1038/nbt923;
RA Larimer F.W., Chain P., Hauser L., Lamerdin J.E., Malfatti S., Do L.,
RA Land M.L., Pelletier D.A., Beatty J.T., Lang A.S., Tabita F.R.,
RA Gibson J.L., Hanson T.E., Bobst C., Torres y Torres J.L., Peres C.,
RA Harrison F.H., Gibson J., Harwood C.S.;
RT "Complete genome sequence of the metabolically versatile photosynthetic
RT bacterium Rhodopseudomonas palustris.";
RL Nat. Biotechnol. 22:55-61(2004).
CC -!- FUNCTION: Catalyzes the formation of the isocyclic ring in chlorophyll
CC biosynthesis. Mediates the cyclase reaction, which results in the
CC formation of divinylprotochlorophyllide (Pchlide) characteristic of all
CC chlorophylls from magnesium-protoporphyrin IX 13-monomethyl ester
CC (MgPMME). {ECO:0000255|HAMAP-Rule:MF_01840}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=2 H(+) + Mg-protoporphyrin IX 13-monomethyl ester + 3 NADPH +
CC 3 O2 = 3,8-divinyl protochlorophyllide a + 5 H2O + 3 NADP(+);
CC Xref=Rhea:RHEA:33235, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378,
CC ChEBI:CHEBI:15379, ChEBI:CHEBI:57783, ChEBI:CHEBI:58349,
CC ChEBI:CHEBI:58632, ChEBI:CHEBI:60491; EC=1.14.13.81;
CC Evidence={ECO:0000255|HAMAP-Rule:MF_01840};
CC -!- COFACTOR:
CC Name=Fe cation; Xref=ChEBI:CHEBI:24875;
CC Evidence={ECO:0000255|HAMAP-Rule:MF_01840};
CC -!- PATHWAY: Porphyrin-containing compound metabolism; bacteriochlorophyll
CC biosynthesis (light-independent). {ECO:0000255|HAMAP-Rule:MF_01840}.
CC -!- SIMILARITY: Belongs to the AcsF family. {ECO:0000255|HAMAP-
CC Rule:MF_01840}.
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DR EMBL; BX572597; CAE26993.1; -; Genomic_DNA.
DR RefSeq; WP_011157111.1; NC_005296.1.
DR AlphaFoldDB; Q6N9J7; -.
DR SMR; Q6N9J7; -.
DR STRING; 258594.RPA1552; -.
DR PRIDE; Q6N9J7; -.
DR DNASU; 2691628; -.
DR EnsemblBacteria; CAE26993; CAE26993; RPA1552.
DR GeneID; 66892582; -.
DR KEGG; rpa:RPA1552; -.
DR eggNOG; COG1633; Bacteria.
DR HOGENOM; CLU_048037_0_0_5; -.
DR OMA; FHPIFKW; -.
DR BioCyc; RPAL258594:TX73_RS07910-MON; -.
DR UniPathway; UPA00671; -.
DR Proteomes; UP000001426; Chromosome.
DR GO; GO:0005506; F:iron ion binding; IEA:UniProtKB-UniRule.
DR GO; GO:0048529; F:magnesium-protoporphyrin IX monomethyl ester (oxidative) cyclase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0036070; P:light-independent bacteriochlorophyll biosynthetic process; IEA:UniProtKB-UniPathway.
DR GO; GO:0015979; P:photosynthesis; IEA:UniProtKB-UniRule.
DR CDD; cd01047; ACSF; 1.
DR HAMAP; MF_01840; AcsF; 1.
DR InterPro; IPR008434; AcsF.
DR InterPro; IPR009078; Ferritin-like_SF.
DR InterPro; IPR003251; Rubrerythrin.
DR PANTHER; PTHR31053; PTHR31053; 1.
DR Pfam; PF02915; Rubrerythrin; 1.
DR SUPFAM; SSF47240; SSF47240; 1.
DR TIGRFAMs; TIGR02029; AcsF; 1.
PE 3: Inferred from homology;
KW Bacteriochlorophyll biosynthesis; Chlorophyll biosynthesis; Iron;
KW Metal-binding; NADP; Oxidoreductase; Photosynthesis; Reference proteome.
FT CHAIN 1..365
FT /note="Aerobic magnesium-protoporphyrin IX monomethyl ester
FT [oxidative] cyclase"
FT /id="PRO_0000217534"
SQ SEQUENCE 365 AA; 42483 MW; 4D6E38C1AA35ED7B CRC64;
MIPMEGGAQG ALRTRPDIKG SVDSLNIAKQ DTILTPRFYT TDYAAMDKLD VSLVRAEWTA
MMNELRADYN KSHFKKTDEF VNSDLDKLPP ELRAEFKDFL VSSLTAEFSG CVLYAEIKKR
IKNPEIRELF GLLSRDEARH AGFINEILKD HGIGVDLSFL TKVKKYTYFR PKFIFYATYL
SEKIGYARYI TIYRQMERHP ERRFHPIFKW FERWCNDEFR HGEAFALLMR ADPSLLSGVN
KLWIRFFLLA VFSTMYVRDH MRPAFYEALG VDATDYGMQV FRITTEISKQ VFPVTINLDD
PRFLQNLERL RIAAEKIDRS HSQGLLGKLK RPFYAASAAL AFGRLFLLPA KRNELPRVIG
LRPAW