ID   CYSO_MYCBO              Reviewed;          93 AA.
AC   P0A647; A0A1R3Y065; Q10646; X2BHE1;
DT   15-MAR-2005, integrated into UniProtKB/Swiss-Prot.
DT   23-JAN-2007, sequence version 2.
DT   25-MAY-2022, entry version 81.
DE   RecName: Full=Sulfur carrier protein CysO;
DE   AltName: Full=9.5 kDa culture filtrate antigen cfp10A;
GN   Name=cysO; Synonyms=cfp10A; OrderedLocusNames=BQ2027_MB1370;
OS   Mycobacterium bovis (strain ATCC BAA-935 / AF2122/97).
OC   Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae;
OC   Mycobacterium; Mycobacterium tuberculosis complex.
OX   NCBI_TaxID=233413;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC BAA-935 / AF2122/97;
RX   PubMed=12788972; DOI=10.1073/pnas.1130426100;
RA   Garnier T., Eiglmeier K., Camus J.-C., Medina N., Mansoor H., Pryor M.,
RA   Duthoy S., Grondin S., Lacroix C., Monsempe C., Simon S., Harris B.,
RA   Atkin R., Doggett J., Mayes R., Keating L., Wheeler P.R., Parkhill J.,
RA   Barrell B.G., Cole S.T., Gordon S.V., Hewinson R.G.;
RT   "The complete genome sequence of Mycobacterium bovis.";
RL   Proc. Natl. Acad. Sci. U.S.A. 100:7877-7882(2003).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], AND GENOME REANNOTATION.
RC   STRAIN=ATCC BAA-935 / AF2122/97;
RX   PubMed=28385856; DOI=10.1128/genomea.00157-17;
RA   Malone K.M., Farrell D., Stuber T.P., Schubert O.T., Aebersold R.,
RA   Robbe-Austerman S., Gordon S.V.;
RT   "Updated reference genome sequence and annotation of Mycobacterium bovis
RT   AF2122/97.";
RL   Genome Announc. 5:E00157-E00157(2017).
CC   -!- FUNCTION: In its thiocarboxylated form (CysO-COSH), is the sulfur donor
CC       in the CysM-dependent cysteine biosynthetic pathway. {ECO:0000250}.
CC   -!- PATHWAY: Amino-acid biosynthesis; L-cysteine biosynthesis.
CC   -!- PTM: Thiocarboxylated by MoeZ. {ECO:0000250}.
CC   -!- PTM: A covalent CysO-cysteine adduct is formed by the action of CysM.
CC       The structure of the adduct seems to be first a S-(glycyl)-cysteinate
CC       which leads to a N-(glycyl)-cysteinate after a S->N acyl shift (By
CC       similarity). {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the sulfur carrier protein CysO family.
CC       {ECO:0000305}.
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DR   EMBL; LT708304; SIT99973.1; -; Genomic_DNA.
DR   RefSeq; NP_855024.1; NC_002945.3.
DR   RefSeq; WP_003406910.1; NC_002945.4.
DR   AlphaFoldDB; P0A647; -.
DR   SMR; P0A647; -.
DR   EnsemblBacteria; SIT99973; SIT99973; BQ2027_MB1370.
DR   GeneID; 45425313; -.
DR   PATRIC; fig|233413.5.peg.1502; -.
DR   OMA; CPGIKAR; -.
DR   UniPathway; UPA00136; -.
DR   Proteomes; UP000001419; Chromosome.
DR   GO; GO:0019344; P:cysteine biosynthetic process; IEA:UniProtKB-UniPathway.
DR   Gene3D; 3.10.20.30; -; 1.
DR   InterPro; IPR012675; Beta-grasp_dom_sf.
DR   InterPro; IPR016155; Mopterin_synth/thiamin_S_b.
DR   InterPro; IPR003749; ThiS/MoaD-like.
DR   Pfam; PF02597; ThiS; 1.
DR   SUPFAM; SSF54285; SSF54285; 1.
PE   3: Inferred from homology;
KW   Amino-acid biosynthesis; Cysteine biosynthesis.
FT   CHAIN           1..93
FT                   /note="Sulfur carrier protein CysO"
FT                   /id="PRO_0000159083"
FT   MOD_RES         93
FT                   /note="1-thioglycine; alternate"
FT                   /evidence="ECO:0000250"
FT   MOD_RES         93
FT                   /note="CysO-cysteine adduct; alternate"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   93 AA;  9557 MW;  CA53151BC14742A8 CRC64;
     MNVTVSIPTI LRPHTGGQKS VSASGDTLGA VISDLEANYS GISERLMDPS SPGKLHRFVN
     IYVNDEDVRF SGGLATAIAD GDSVTILPAV AGG