CYSO_MYCTU
ID CYSO_MYCTU Reviewed; 93 AA.
AC P9WP33; L0T9C0; P0A646; Q10646;
DT 16-APR-2014, integrated into UniProtKB/Swiss-Prot.
DT 16-APR-2014, sequence version 1.
DT 03-AUG-2022, entry version 43.
DE RecName: Full=Sulfur carrier protein CysO;
DE AltName: Full=9.5 kDa culture filtrate antigen cfp10A;
GN Name=cysO; Synonyms=cfp10A; OrderedLocusNames=Rv1335; ORFNames=MTCY130.20;
OS Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv).
OC Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae;
OC Mycobacterium; Mycobacterium tuberculosis complex.
OX NCBI_TaxID=83332;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=ATCC 25618 / H37Rv;
RA Oettinger T.;
RL Submitted (AUG-1998) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 25618 / H37Rv;
RX PubMed=9634230; DOI=10.1038/31159;
RA Cole S.T., Brosch R., Parkhill J., Garnier T., Churcher C.M., Harris D.E.,
RA Gordon S.V., Eiglmeier K., Gas S., Barry C.E. III, Tekaia F., Badcock K.,
RA Basham D., Brown D., Chillingworth T., Connor R., Davies R.M., Devlin K.,
RA Feltwell T., Gentles S., Hamlin N., Holroyd S., Hornsby T., Jagels K.,
RA Krogh A., McLean J., Moule S., Murphy L.D., Oliver S., Osborne J.,
RA Quail M.A., Rajandream M.A., Rogers J., Rutter S., Seeger K., Skelton S.,
RA Squares S., Squares R., Sulston J.E., Taylor K., Whitehead S.,
RA Barrell B.G.;
RT "Deciphering the biology of Mycobacterium tuberculosis from the complete
RT genome sequence.";
RL Nature 393:537-544(1998).
RN [3]
RP IDENTIFICATION BY MASS SPECTROMETRY.
RC STRAIN=ATCC 25618 / H37Rv;
RX PubMed=11681203;
RX DOI=10.1002/1615-9861(200104)1:4<494::aid-prot494>3.0.co;2-4;
RA Mattow J., Jungblut P.R., Mueller E.-C., Kaufmann S.H.E.;
RT "Identification of acidic, low molecular mass proteins of Mycobacterium
RT tuberculosis strain H37Rv by matrix-assisted laser desorption/ionization
RT and electrospray ionization mass spectrometry.";
RL Proteomics 1:494-507(2001).
RN [4]
RP INDUCTION.
RC STRAIN=ATCC 25618 / H37Rv;
RX PubMed=12123450; DOI=10.1046/j.1365-2958.2002.03005.x;
RA Manganelli R., Voskuil M.I., Schoolnik G.K., Dubnau E., Gomez M., Smith I.;
RT "Role of the extracytoplasmic-function sigma factor sigma(H) in
RT Mycobacterium tuberculosis global gene expression.";
RL Mol. Microbiol. 45:365-374(2002).
RN [5]
RP FUNCTION, AND THIOCARBOXYLATION AT GLY-93 BY MOEZ.
RC STRAIN=ATCC 25618 / H37Rv;
RX PubMed=16104727; DOI=10.1021/ja053476x;
RA Burns K.E., Baumgart S., Dorrestein P.C., Zhai H., McLafferty F.W.,
RA Begley T.P.;
RT "Reconstitution of a new cysteine biosynthetic pathway in Mycobacterium
RT tuberculosis.";
RL J. Am. Chem. Soc. 127:11602-11603(2005).
RN [6]
RP FUNCTION, AND KINETIC STUDIES.
RX PubMed=18842002; DOI=10.1021/bi8013664;
RA O'Leary S.E., Jurgenson C.T., Ealick S.E., Begley T.P.;
RT "O-phospho-L-serine and the thiocarboxylated sulfur carrier protein CysO-
RT COSH are substrates for CysM, a cysteine synthase from Mycobacterium
RT tuberculosis.";
RL Biochemistry 47:11606-11615(2008).
RN [7]
RP FUNCTION, MASS SPECTROMETRY, AND PTM.
RC STRAIN=ATCC 25618 / H37Rv;
RX PubMed=18799456; DOI=10.1074/jbc.m804877200;
RA Agren D., Schnell R., Oehlmann W., Singh M., Schneider G.;
RT "Cysteine synthase (CysM) of Mycobacterium tuberculosis is an O-
RT phosphoserine sulfhydrylase: evidence for an alternative cysteine
RT biosynthesis pathway in mycobacteria.";
RL J. Biol. Chem. 283:31567-31574(2008).
RN [8]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC STRAIN=ATCC 25618 / H37Rv;
RX PubMed=21969609; DOI=10.1074/mcp.m111.011627;
RA Kelkar D.S., Kumar D., Kumar P., Balakrishnan L., Muthusamy B., Yadav A.K.,
RA Shrivastava P., Marimuthu A., Anand S., Sundaram H., Kingsbury R.,
RA Harsha H.C., Nair B., Prasad T.S., Chauhan D.S., Katoch K., Katoch V.M.,
RA Kumar P., Chaerkady R., Ramachandran S., Dash D., Pandey A.;
RT "Proteogenomic analysis of Mycobacterium tuberculosis by high resolution
RT mass spectrometry.";
RL Mol. Cell. Proteomics 10:M111.011627-M111.011627(2011).
RN [9]
RP X-RAY CRYSTALLOGRAPHY (1.53 ANGSTROMS) OF NATIVE PROTEIN AND COMPLEX WITH
RP PROTEIN CYSM.
RX PubMed=18771296; DOI=10.1021/bi800915j;
RA Jurgenson C.T., Burns K.E., Begley T.P., Ealick S.E.;
RT "Crystal structure of a sulfur carrier protein complex found in the
RT cysteine biosynthetic pathway of Mycobacterium tuberculosis.";
RL Biochemistry 47:10354-10364(2008).
CC -!- FUNCTION: In its thiocarboxylated form (CysO-COSH), is the sulfur donor
CC in the CysM-dependent cysteine biosynthetic pathway. May be of
CC particular importance for cysteine biosynthesis in the persistent phase
CC of M.tuberculosis. {ECO:0000269|PubMed:16104727,
CC ECO:0000269|PubMed:18799456, ECO:0000269|PubMed:18842002}.
CC -!- PATHWAY: Amino-acid biosynthesis; L-cysteine biosynthesis.
CC -!- INDUCTION: Up-regulated under oxidative stress conditions.
CC {ECO:0000269|PubMed:12123450}.
CC -!- PTM: Thiocarboxylated by MoeZ. {ECO:0000269|PubMed:16104727}.
CC -!- PTM: A covalent CysO-cysteine adduct is formed by the action of CysM.
CC The structure of the adduct seems to be first a S-(glycyl)-cysteinate
CC which leads to a N-(glycyl)-cysteinate after a S->N acyl shift.
CC {ECO:0000269|PubMed:18799456}.
CC -!- MASS SPECTROMETRY: Mass=9557.5; Method=Unknown; Note=Native form.;
CC Evidence={ECO:0000269|PubMed:18799456};
CC -!- MASS SPECTROMETRY: Mass=9573.8; Method=Unknown; Note=Thiocarboxylated
CC form.; Evidence={ECO:0000269|PubMed:18799456};
CC -!- MASS SPECTROMETRY: Mass=9659.6; Method=Unknown; Note=CysO-cysteine
CC adduct.; Evidence={ECO:0000269|PubMed:18799456};
CC -!- SIMILARITY: Belongs to the sulfur carrier protein CysO family.
CC {ECO:0000305}.
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DR EMBL; AJ007738; CAA07637.1; -; Genomic_DNA.
DR EMBL; AL123456; CCP44093.1; -; Genomic_DNA.
DR PIR; C70771; C70771.
DR RefSeq; NP_215851.1; NC_000962.3.
DR RefSeq; WP_003406910.1; NZ_NVQJ01000031.1.
DR PDB; 3DWG; X-ray; 1.53 A; C=1-93.
DR PDB; 3DWM; X-ray; 2.69 A; A/B=1-93.
DR PDBsum; 3DWG; -.
DR PDBsum; 3DWM; -.
DR AlphaFoldDB; P9WP33; -.
DR SMR; P9WP33; -.
DR STRING; 83332.Rv1335; -.
DR PaxDb; P9WP33; -.
DR DNASU; 886879; -.
DR GeneID; 45425313; -.
DR GeneID; 886879; -.
DR KEGG; mtu:Rv1335; -.
DR TubercuList; Rv1335; -.
DR eggNOG; COG1977; Bacteria.
DR OMA; CPGIKAR; -.
DR PhylomeDB; P9WP33; -.
DR BioCyc; MetaCyc:G185E-5514-MON; -.
DR Reactome; R-MTU-936654; Cysteine synthesis from O-phosphoserine.
DR UniPathway; UPA00136; -.
DR Proteomes; UP000001584; Chromosome.
DR GO; GO:0005829; C:cytosol; TAS:Reactome.
DR GO; GO:0032991; C:protein-containing complex; IDA:MTBBASE.
DR GO; GO:0019344; P:cysteine biosynthetic process; IDA:MTBBASE.
DR Gene3D; 3.10.20.30; -; 1.
DR InterPro; IPR012675; Beta-grasp_dom_sf.
DR InterPro; IPR016155; Mopterin_synth/thiamin_S_b.
DR InterPro; IPR003749; ThiS/MoaD-like.
DR Pfam; PF02597; ThiS; 1.
DR SUPFAM; SSF54285; SSF54285; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Amino-acid biosynthesis; Cysteine biosynthesis;
KW Reference proteome.
FT CHAIN 1..93
FT /note="Sulfur carrier protein CysO"
FT /id="PRO_0000159082"
FT MOD_RES 93
FT /note="1-thioglycine; alternate"
FT /evidence="ECO:0000269|PubMed:16104727"
FT MOD_RES 93
FT /note="CysO-cysteine adduct; alternate"
FT /evidence="ECO:0000269|PubMed:18799456"
FT STRAND 3..6
FT /evidence="ECO:0007829|PDB:3DWG"
FT HELIX 9..14
FT /evidence="ECO:0007829|PDB:3DWG"
FT TURN 15..17
FT /evidence="ECO:0007829|PDB:3DWG"
FT STRAND 19..23
FT /evidence="ECO:0007829|PDB:3DWG"
FT HELIX 28..38
FT /evidence="ECO:0007829|PDB:3DWG"
FT HELIX 42..46
FT /evidence="ECO:0007829|PDB:3DWG"
FT STRAND 49..51
FT /evidence="ECO:0007829|PDB:3DWG"
FT STRAND 59..63
FT /evidence="ECO:0007829|PDB:3DWG"
FT HELIX 68..70
FT /evidence="ECO:0007829|PDB:3DWG"
FT HELIX 73..75
FT /evidence="ECO:0007829|PDB:3DWG"
FT STRAND 83..88
FT /evidence="ECO:0007829|PDB:3DWG"
SQ SEQUENCE 93 AA; 9557 MW; CA53151BC14742A8 CRC64;
MNVTVSIPTI LRPHTGGQKS VSASGDTLGA VISDLEANYS GISERLMDPS SPGKLHRFVN
IYVNDEDVRF SGGLATAIAD GDSVTILPAV AGG