ACSF_RUBGE
ID ACSF_RUBGE Reviewed; 358 AA.
AC P0DJN9; Q8VPB5; Q9JPB1;
DT 05-SEP-2012, integrated into UniProtKB/Swiss-Prot.
DT 05-SEP-2012, sequence version 1.
DT 03-AUG-2022, entry version 26.
DE RecName: Full=Aerobic magnesium-protoporphyrin IX monomethyl ester [oxidative] cyclase {ECO:0000255|HAMAP-Rule:MF_01840};
DE Short=Aerobic Mg-protoporphyrin IX monomethyl ester oxidative cyclase {ECO:0000255|HAMAP-Rule:MF_01840};
DE EC=1.14.13.81 {ECO:0000255|HAMAP-Rule:MF_01840};
GN Name=acsF {ECO:0000255|HAMAP-Rule:MF_01840};
OS Rubrivivax gelatinosus (Rhodocyclus gelatinosus) (Rhodopseudomonas
OS gelatinosa).
OC Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales; Rubrivivax.
OX NCBI_TaxID=28068;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, AND DISRUPTION PHENOTYPE.
RC STRAIN=S1;
RX PubMed=11790744; DOI=10.1128/jb.184.3.746-753.2002;
RA Pinta V., Picaud M., Reiss-Husson F., Astier C.;
RT "Rubrivivax gelatinosus acsF (previously orf358) codes for a conserved,
RT putative binuclear-iron-cluster-containing protein involved in aerobic
RT oxidative cyclization of Mg-protoporphyrin IX monomethylester.";
RL J. Bacteriol. 184:746-753(2002).
CC -!- FUNCTION: Catalyzes the formation of the isocyclic ring in chlorophyll
CC biosynthesis in aerobic conditions. Mediates the cyclase reaction,
CC which results in the formation of divinylprotochlorophyllide (Pchlide)
CC characteristic of all chlorophylls from magnesium-protoporphyrin IX 13-
CC monomethyl ester (MgPMME). {ECO:0000269|PubMed:11790744}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=2 H(+) + Mg-protoporphyrin IX 13-monomethyl ester + 3 NADPH +
CC 3 O2 = 3,8-divinyl protochlorophyllide a + 5 H2O + 3 NADP(+);
CC Xref=Rhea:RHEA:33235, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378,
CC ChEBI:CHEBI:15379, ChEBI:CHEBI:57783, ChEBI:CHEBI:58349,
CC ChEBI:CHEBI:58632, ChEBI:CHEBI:60491; EC=1.14.13.81;
CC Evidence={ECO:0000255|HAMAP-Rule:MF_01840};
CC -!- COFACTOR:
CC Name=Fe cation; Xref=ChEBI:CHEBI:24875;
CC Evidence={ECO:0000255|HAMAP-Rule:MF_01840};
CC -!- PATHWAY: Porphyrin-containing compound metabolism; chlorophyll
CC biosynthesis.
CC -!- DISRUPTION PHENOTYPE: No phenotype under photosynthesis or low-aeration
CC respiratory growth conditions. In contrast, under highly aerated
CC respiratory growth conditions, the absence of AcsF leads to an
CC accumulation of MgPMME. {ECO:0000269|PubMed:11790744}.
CC -!- SIMILARITY: Belongs to the AcsF family. {ECO:0000255|HAMAP-
CC Rule:MF_01840}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; AY234384; AAL25840.2; -; Genomic_DNA.
DR AlphaFoldDB; P0DJN9; -.
DR SMR; P0DJN9; -.
DR BioCyc; MetaCyc:MON-13266; -.
DR BRENDA; 1.14.13.81; 5401.
DR UniPathway; UPA00668; -.
DR GO; GO:0005506; F:iron ion binding; IEA:UniProtKB-UniRule.
DR GO; GO:0048529; F:magnesium-protoporphyrin IX monomethyl ester (oxidative) cyclase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0030494; P:bacteriochlorophyll biosynthetic process; IEA:UniProtKB-UniRule.
DR GO; GO:0015979; P:photosynthesis; IEA:UniProtKB-UniRule.
DR CDD; cd01047; ACSF; 1.
DR HAMAP; MF_01840; AcsF; 1.
DR InterPro; IPR008434; AcsF.
DR InterPro; IPR009078; Ferritin-like_SF.
DR InterPro; IPR003251; Rubrerythrin.
DR PANTHER; PTHR31053; PTHR31053; 1.
DR Pfam; PF02915; Rubrerythrin; 1.
DR SUPFAM; SSF47240; SSF47240; 1.
DR TIGRFAMs; TIGR02029; AcsF; 1.
PE 3: Inferred from homology;
KW Bacteriochlorophyll biosynthesis; Chlorophyll biosynthesis; Iron;
KW Metal-binding; NADP; Oxidoreductase; Photosynthesis.
FT CHAIN 1..358
FT /note="Aerobic magnesium-protoporphyrin IX monomethyl ester
FT [oxidative] cyclase"
FT /id="PRO_0000217533"
SQ SEQUENCE 358 AA; 41513 MW; 299E24EFFD4BFD9C CRC64;
MLATPTIESP EEAARRAKES TLLSPRFYTT DYAAMNAIDV SSIRAEWDAM LAEYEGDNNH
DHFQRTPEFA QEVAERFSQV SPELRQEFLD FLVSSVTSEF SGCVLYNEIQ KNVENPDVKA
LMRYMARNES RHAGFINQAL RDFGLGINLG GLKRTKAYTY FKPKYIFYAT YLSEKIGYAR
YITIYRQLER HPDKRFHPIF RWFERWCNDE FRHGESFALI LRAHPHLISG ANLLWVRFFL
LAVYATMYVR DHMRPQLHEA MGLESTDYDY RVFQITNEIS KQVFPISLDI DHQAFRAGME
RLVRVKTKVD AAKARGGLVG RLQQAAWAAA GAATFARMYL IPVRRHALPA QVRMAPAW