ID   ACSF_RUBGI              Reviewed;         358 AA.
AC   I0HUK7; Q8VPB5; Q9JPB1;
DT   05-SEP-2012, integrated into UniProtKB/Swiss-Prot.
DT   13-JUN-2012, sequence version 1.
DT   25-MAY-2022, entry version 43.
DE   RecName: Full=Aerobic magnesium-protoporphyrin IX monomethyl ester [oxidative] cyclase {ECO:0000255|HAMAP-Rule:MF_01840};
DE            Short=Aerobic Mg-protoporphyrin IX monomethyl ester oxidative cyclase {ECO:0000255|HAMAP-Rule:MF_01840};
DE            EC=1.14.13.81 {ECO:0000255|HAMAP-Rule:MF_01840};
GN   Name=acsF {ECO:0000255|HAMAP-Rule:MF_01840}; OrderedLocusNames=RGE_33550;
OS   Rubrivivax gelatinosus (strain NBRC 100245 / IL144).
OC   Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales; Rubrivivax.
OX   NCBI_TaxID=983917;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=NBRC 100245 / IL144;
RA   Nagashima K.V., Igarashi N., Harada J., Nagashima S., Matsuura K.,
RA   Shimada K.;
RT   "Determination of nucleotide sequences of Rubrivivax gelatinosus
RT   Photosynthetic genes.";
RL   Submitted (NOV-1999) to the EMBL/GenBank/DDBJ databases.
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=NBRC 100245 / IL144;
RX   PubMed=22689232; DOI=10.1128/jb.00511-12;
RA   Nagashima S., Kamimura A., Shimizu T., Nakamura-Isaki S., Aono E.,
RA   Sakamoto K., Ichikawa N., Nakazawa H., Sekine M., Yamazaki S., Fujita N.,
RA   Shimada K., Hanada S., Nagashima K.V.;
RT   "Complete genome sequence of phototrophic betaproteobacterium Rubrivivax
RT   gelatinosus IL144.";
RL   J. Bacteriol. 194:3541-3542(2012).
CC   -!- FUNCTION: Catalyzes the formation of the isocyclic ring in chlorophyll
CC       biosynthesis in aerobic conditions. Mediates the cyclase reaction,
CC       which results in the formation of divinylprotochlorophyllide (Pchlide)
CC       characteristic of all chlorophylls from magnesium-protoporphyrin IX 13-
CC       monomethyl ester (MgPMME) (By similarity). {ECO:0000250}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=2 H(+) + Mg-protoporphyrin IX 13-monomethyl ester + 3 NADPH +
CC         3 O2 = 3,8-divinyl protochlorophyllide a + 5 H2O + 3 NADP(+);
CC         Xref=Rhea:RHEA:33235, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378,
CC         ChEBI:CHEBI:15379, ChEBI:CHEBI:57783, ChEBI:CHEBI:58349,
CC         ChEBI:CHEBI:58632, ChEBI:CHEBI:60491; EC=1.14.13.81;
CC         Evidence={ECO:0000255|HAMAP-Rule:MF_01840};
CC   -!- COFACTOR:
CC       Name=Fe cation; Xref=ChEBI:CHEBI:24875;
CC         Evidence={ECO:0000255|HAMAP-Rule:MF_01840};
CC   -!- PATHWAY: Porphyrin-containing compound metabolism; chlorophyll
CC       biosynthesis.
CC   -!- SIMILARITY: Belongs to the AcsF family. {ECO:0000255|HAMAP-
CC       Rule:MF_01840}.
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DR   EMBL; AB034704; BAA94050.1; -; Genomic_DNA.
DR   EMBL; AP012320; BAL96694.1; -; Genomic_DNA.
DR   PIR; T50897; T50897.
DR   RefSeq; WP_014429555.1; NC_017075.1.
DR   AlphaFoldDB; I0HUK7; -.
DR   SMR; I0HUK7; -.
DR   STRING; 983917.RGE_33550; -.
DR   EnsemblBacteria; BAL96694; BAL96694; RGE_33550.
DR   KEGG; rge:RGE_33550; -.
DR   PATRIC; fig|983917.3.peg.3281; -.
DR   eggNOG; COG1633; Bacteria.
DR   HOGENOM; CLU_048037_0_0_4; -.
DR   OMA; FHPIFKW; -.
DR   OrthoDB; 366541at2; -.
DR   BRENDA; 1.14.13.81; 5401.
DR   UniPathway; UPA00668; -.
DR   Proteomes; UP000007883; Chromosome.
DR   GO; GO:0005506; F:iron ion binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0048529; F:magnesium-protoporphyrin IX monomethyl ester (oxidative) cyclase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0030494; P:bacteriochlorophyll biosynthetic process; IEA:UniProtKB-UniRule.
DR   GO; GO:0015979; P:photosynthesis; IEA:UniProtKB-UniRule.
DR   CDD; cd01047; ACSF; 1.
DR   HAMAP; MF_01840; AcsF; 1.
DR   InterPro; IPR008434; AcsF.
DR   InterPro; IPR009078; Ferritin-like_SF.
DR   InterPro; IPR003251; Rubrerythrin.
DR   PANTHER; PTHR31053; PTHR31053; 1.
DR   Pfam; PF02915; Rubrerythrin; 1.
DR   SUPFAM; SSF47240; SSF47240; 1.
DR   TIGRFAMs; TIGR02029; AcsF; 1.
PE   3: Inferred from homology;
KW   Bacteriochlorophyll biosynthesis; Chlorophyll biosynthesis; Iron;
KW   Metal-binding; NADP; Oxidoreductase; Photosynthesis; Reference proteome.
FT   CHAIN           1..358
FT                   /note="Aerobic magnesium-protoporphyrin IX monomethyl ester
FT                   [oxidative] cyclase"
FT                   /id="PRO_0000418665"
SQ   SEQUENCE   358 AA;  41516 MW;  B08334479E52F8AE CRC64;
     MLATPTIESP EEAARRAKES TLLSPRFYTT DYAAMNAIDV SSIRAEWDAM LAEYEGDNNH
     DHFQRTPEFP QEVAERFSQV SPELRQEFLD FLVSSVTSEF SGCVLYNEIQ KNVENPDVKA
     LMRYMARDES RHAGFINQAL RDFGLGIDLG GLKRTKAYTY FKPKYIFYAT YLSEKIGYAR
     YITIYRQLER HPDKRFHPIF RWFERWCNDE FRHGESFALI LRAHPHLITG PNLLWVRFFL
     LAVYATMYVR DHMRPLLHEA MGLESTDYDY RVFQITNEIS KQVFPISLDI DHPAFRAGME
     RLVHVKTKVD AAKARGGLVG RLQQAAWAAA GAATFARMYL IPVRRHALPA QVRMAPAW