ID   CYSP3_SOLLC             Reviewed;         356 AA.
AC   Q40143;
DT   01-NOV-1997, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1997, sequence version 1.
DT   03-AUG-2022, entry version 110.
DE   RecName: Full=Cysteine proteinase 3;
DE            EC=3.4.22.-;
DE   Flags: Precursor;
GN   Name=CYP-3;
OS   Solanum lycopersicum (Tomato) (Lycopersicon esculentum).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   asterids; lamiids; Solanales; Solanaceae; Solanoideae; Solaneae; Solanum;
OC   Solanum subgen. Lycopersicon.
OX   NCBI_TaxID=4081;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RC   STRAIN=cv. Ailsa Craig; TISSUE=Leaf;
RX   PubMed=8624407; DOI=10.1007/bf00019009;
RA   Drake R., John I., Farrell A., Cooper W., Schuch W., Grierson D.;
RT   "Isolation and analysis of cDNAs encoding tomato cysteine proteases
RT   expressed during leaf senescence.";
RL   Plant Mol. Biol. 30:755-767(1996).
CC   -!- SUBCELLULAR LOCATION: Vacuole {ECO:0000305}.
CC   -!- TISSUE SPECIFICITY: Predominantly expressed in stem and root.
CC   -!- SIMILARITY: Belongs to the peptidase C1 family. {ECO:0000255|PROSITE-
CC       ProRule:PRU10088, ECO:0000255|PROSITE-ProRule:PRU10089,
CC       ECO:0000255|PROSITE-ProRule:PRU10090}.
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DR   EMBL; Z48736; CAA88629.1; -; mRNA.
DR   PIR; S66348; S66348.
DR   RefSeq; NP_001296313.1; NM_001309384.1.
DR   AlphaFoldDB; Q40143; -.
DR   SMR; Q40143; -.
DR   STRING; 4081.Solyc07g041900.2.1; -.
DR   MEROPS; C01.163; -.
DR   PaxDb; Q40143; -.
DR   PRIDE; Q40143; -.
DR   ProMEX; Q40143; -.
DR   GeneID; 101252505; -.
DR   KEGG; sly:101252505; -.
DR   eggNOG; KOG1543; Eukaryota.
DR   HOGENOM; CLU_012184_1_2_1; -.
DR   InParanoid; Q40143; -.
DR   OrthoDB; 1275401at2759; -.
DR   PhylomeDB; Q40143; -.
DR   Proteomes; UP000004994; Unplaced.
DR   GO; GO:0005615; C:extracellular space; IBA:GO_Central.
DR   GO; GO:0005764; C:lysosome; IBA:GO_Central.
DR   GO; GO:0004197; F:cysteine-type endopeptidase activity; IBA:GO_Central.
DR   GO; GO:0051603; P:proteolysis involved in protein catabolic process; IBA:GO_Central.
DR   CDD; cd02248; Peptidase_C1A; 1.
DR   InterPro; IPR038765; Papain-like_cys_pep_sf.
DR   InterPro; IPR025661; Pept_asp_AS.
DR   InterPro; IPR000169; Pept_cys_AS.
DR   InterPro; IPR025660; Pept_his_AS.
DR   InterPro; IPR000668; Peptidase_C1A_C.
DR   InterPro; IPR039417; Peptidase_C1A_papain-like.
DR   InterPro; IPR013201; Prot_inhib_I29.
DR   Pfam; PF08246; Inhibitor_I29; 1.
DR   Pfam; PF00112; Peptidase_C1; 1.
DR   PRINTS; PR00705; PAPAIN.
DR   SMART; SM00848; Inhibitor_I29; 1.
DR   SMART; SM00645; Pept_C1; 1.
DR   SUPFAM; SSF54001; SSF54001; 1.
DR   PROSITE; PS00640; THIOL_PROTEASE_ASN; 1.
DR   PROSITE; PS00139; THIOL_PROTEASE_CYS; 1.
DR   PROSITE; PS00639; THIOL_PROTEASE_HIS; 1.
PE   2: Evidence at transcript level;
KW   Disulfide bond; Glycoprotein; Hydrolase; Protease; Reference proteome;
KW   Signal; Thiol protease; Vacuole; Zymogen.
FT   SIGNAL          1..16
FT                   /evidence="ECO:0000255"
FT   PROPEP          17..138
FT                   /note="Activation peptide"
FT                   /evidence="ECO:0000255"
FT                   /id="PRO_0000026426"
FT   CHAIN           139..356
FT                   /note="Cysteine proteinase 3"
FT                   /id="PRO_0000026427"
FT   ACT_SITE        163
FT                   /evidence="ECO:0000250"
FT   ACT_SITE        303
FT                   /evidence="ECO:0000250"
FT   ACT_SITE        323
FT                   /evidence="ECO:0000250"
FT   CARBOHYD        123
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        252
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   DISULFID        160..203
FT                   /evidence="ECO:0000250"
FT   DISULFID        194..236
FT                   /evidence="ECO:0000250"
FT   DISULFID        294..349
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   356 AA;  38945 MW;  BE176A7C74BCDD44 CRC64;
     MSRLSLVLIL VAGLFATALA GPATFADKNP IRQVVFPDEL ENGILQVVGQ TRSALSFARF
     AIRHRKRYDS VEEIKQRFEI FLDNLKMIRS HNRKGLSYKL GINEFTDLTW DEFRKHKLGA
     SQNCSATTKG NLKLTNVVLP ETKDWRKDGI VSPVKAQGKC GSCWTFSTTG ALEAAYAQAF
     GKGISLSEQQ LVDCAGAFNN FGCNGGLPSQ AFEYIKFNGG LDTEEAYPYT GKNGICKFSQ
     ANIGVKVISS VNITLGAEYE LKYAVALVRP VSVAFEVVKG FKQYKSGVYA STECGDTPMD
     VNHAVLAVGY GVENGTPYWL IKNSWGADWG EDGYFKMEMG KNMCGVATCA SYPIVA