CYSP5_DICDI
ID CYSP5_DICDI Reviewed; 344 AA.
AC P54640; Q558T9; Q8MNB1;
DT 01-OCT-1996, integrated into UniProtKB/Swiss-Prot.
DT 04-DEC-2007, sequence version 2.
DT 03-AUG-2022, entry version 133.
DE RecName: Full=Cysteine proteinase 5;
DE EC=3.4.22.-;
DE Flags: Precursor;
GN Name=cprE; Synonyms=CP5; ORFNames=DDB_G0272815;
OS Dictyostelium discoideum (Slime mold).
OC Eukaryota; Amoebozoa; Evosea; Eumycetozoa; Dictyostelia; Dictyosteliales;
OC Dictyosteliaceae; Dictyostelium.
OX NCBI_TaxID=44689;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC STRAIN=AX4;
RX PubMed=7499424; DOI=10.1074/jbc.270.48.28938;
RA Souza G.M., Hirai J., Mehta D.P., Freeze H.H.;
RT "Identification of two novel Dictyostelium discoideum cysteine proteinases
RT that carry N-acetylglucosamine-1-P-modification.";
RL J. Biol. Chem. 270:28938-28945(1995).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=AX4;
RX PubMed=12097910; DOI=10.1038/nature00847;
RA Gloeckner G., Eichinger L., Szafranski K., Pachebat J.A., Bankier A.T.,
RA Dear P.H., Lehmann R., Baumgart C., Parra G., Abril J.F., Guigo R.,
RA Kumpf K., Tunggal B., Cox E.C., Quail M.A., Platzer M., Rosenthal A.,
RA Noegel A.A.;
RT "Sequence and analysis of chromosome 2 of Dictyostelium discoideum.";
RL Nature 418:79-85(2002).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=AX4;
RX PubMed=15875012; DOI=10.1038/nature03481;
RA Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R.,
RA Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B.,
RA Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T.,
RA Lehmann R., Hamlin N., Davies R., Gaudet P., Fey P., Pilcher K., Chen G.,
RA Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N.,
RA Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E.,
RA Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N.,
RA Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D.,
RA Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T.,
RA Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D.,
RA Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A.,
RA Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M.,
RA Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A.,
RA Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y.,
RA Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C.,
RA Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R.,
RA Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.;
RT "The genome of the social amoeba Dictyostelium discoideum.";
RL Nature 435:43-57(2005).
CC -!- SUBCELLULAR LOCATION: Lysosome.
CC -!- DEVELOPMENTAL STAGE: Present in the vegetative phase and decreases with
CC the start development, reappears in low levels when the fruiting body
CC is formed.
CC -!- PTM: Glycosylated; contains GlcNAc-alpha-1-P-Ser residues.
CC -!- SIMILARITY: Belongs to the peptidase C1 family. {ECO:0000255|PROSITE-
CC ProRule:PRU10088, ECO:0000255|PROSITE-ProRule:PRU10089,
CC ECO:0000255|PROSITE-ProRule:PRU10090}.
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DR EMBL; L36205; AAA92018.1; -; mRNA.
DR EMBL; AAFI02000008; EAL71045.1; -; Genomic_DNA.
DR RefSeq; XP_644977.1; XM_639885.1.
DR AlphaFoldDB; P54640; -.
DR SMR; P54640; -.
DR BioGRID; 1243657; 1.
DR STRING; 44689.DDB0185092; -.
DR MEROPS; C01.A57; -.
DR MEROPS; I29.003; -.
DR PaxDb; P54640; -.
DR EnsemblProtists; EAL71045; EAL71045; DDB_G0272815.
DR GeneID; 8618654; -.
DR KEGG; ddi:DDB_G0272815; -.
DR dictyBase; DDB_G0272815; cprE.
DR eggNOG; KOG1543; Eukaryota.
DR HOGENOM; CLU_012184_1_2_1; -.
DR InParanoid; P54640; -.
DR OMA; PVGNEKA; -.
DR PhylomeDB; P54640; -.
DR Reactome; R-DDI-1442490; Collagen degradation.
DR Reactome; R-DDI-1474228; Degradation of the extracellular matrix.
DR Reactome; R-DDI-2132295; MHC class II antigen presentation.
DR Reactome; R-DDI-5683826; Surfactant metabolism.
DR Reactome; R-DDI-6798695; Neutrophil degranulation.
DR PRO; PR:P54640; -.
DR Proteomes; UP000002195; Chromosome 2.
DR GO; GO:0005615; C:extracellular space; IBA:GO_Central.
DR GO; GO:0005764; C:lysosome; IBA:GO_Central.
DR GO; GO:0004197; F:cysteine-type endopeptidase activity; IBA:GO_Central.
DR GO; GO:0006955; P:immune response; IBA:GO_Central.
DR GO; GO:0051603; P:proteolysis involved in protein catabolic process; IBA:GO_Central.
DR CDD; cd02248; Peptidase_C1A; 1.
DR InterPro; IPR038765; Papain-like_cys_pep_sf.
DR InterPro; IPR025661; Pept_asp_AS.
DR InterPro; IPR000169; Pept_cys_AS.
DR InterPro; IPR025660; Pept_his_AS.
DR InterPro; IPR000668; Peptidase_C1A_C.
DR InterPro; IPR039417; Peptidase_C1A_papain-like.
DR InterPro; IPR013201; Prot_inhib_I29.
DR Pfam; PF08246; Inhibitor_I29; 1.
DR Pfam; PF00112; Peptidase_C1; 1.
DR PRINTS; PR00705; PAPAIN.
DR SMART; SM00848; Inhibitor_I29; 1.
DR SMART; SM00645; Pept_C1; 1.
DR SUPFAM; SSF54001; SSF54001; 1.
DR PROSITE; PS00640; THIOL_PROTEASE_ASN; 1.
DR PROSITE; PS00139; THIOL_PROTEASE_CYS; 1.
DR PROSITE; PS00639; THIOL_PROTEASE_HIS; 1.
PE 2: Evidence at transcript level;
KW Disulfide bond; Glycoprotein; Hydrolase; Lysosome; Phosphoprotein;
KW Protease; Reference proteome; Signal; Thiol protease; Zymogen.
FT SIGNAL 1..17
FT /evidence="ECO:0000255"
FT PROPEP 18..111
FT /note="Activation peptide"
FT /evidence="ECO:0000255"
FT /id="PRO_0000026364"
FT CHAIN 112..344
FT /note="Cysteine proteinase 5"
FT /id="PRO_0000026365"
FT ACT_SITE 136
FT /evidence="ECO:0000250"
FT ACT_SITE 272
FT /evidence="ECO:0000250"
FT ACT_SITE 311
FT /evidence="ECO:0000250"
FT CARBOHYD 297
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT DISULFID 133..174
FT /evidence="ECO:0000250"
FT DISULFID 167..207
FT /evidence="ECO:0000250"
FT DISULFID 265..333
FT /evidence="ECO:0000250"
FT CONFLICT 54
FT /note="K -> T (in Ref. 1; AAA92018)"
FT /evidence="ECO:0000305"
FT CONFLICT 108..110
FT /note="FTT -> HTN (in Ref. 1; AAA92018)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 344 AA; 37237 MW; 1E77FBA4BD8337B7 CRC64;
MKVLSFLCVL LVSVATAKQQ FSELQYRNAF TDWMITHQKS YTSEEFGARY NIFKANMDYV
QQWNSKGSET VLGLNNFADI TNEEYRNTYL GTKFDASSLI GTQEEKVFTT SSAASKDWRS
EGAVTPVKNQ GQCGGCWSFS TTGSTEGAHF QSKGELVSLS EQNLIDCSTE NSGCDGGLMT
YAFEYIINNN GIDTESSYPY KAENGKCEYK SENSGATLSS YKTVTAGSES SLESAVNVNP
VSVAIDASHQ SFQLYTSGIY YEPECSSENL DHGVLAVGYG SGSGSSSGQS SGQSSGNLSA
SSSNEYWIVK NSWGTSWGIE GYILMSRNRD NNCGIASSAS FPVV
