ID   CYSPL_SOLLC             Reviewed;         346 AA.
AC   P20721;
DT   01-FEB-1991, integrated into UniProtKB/Swiss-Prot.
DT   01-FEB-1991, sequence version 1.
DT   03-AUG-2022, entry version 100.
DE   RecName: Full=Low-temperature-induced cysteine proteinase;
DE            EC=3.4.22.-;
DE   Flags: Precursor; Fragment;
OS   Solanum lycopersicum (Tomato) (Lycopersicon esculentum).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   asterids; lamiids; Solanales; Solanaceae; Solanoideae; Solaneae; Solanum;
OC   Solanum subgen. Lycopersicon.
OX   NCBI_TaxID=4081;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RC   STRAIN=cv. VFNT Cherry;
RX   PubMed=16666159; DOI=10.1104/pp.87.2.431;
RA   Schaffer M.A., Fischer R.L.;
RT   "Analysis of mRNAs that accumulate in response to low temperature
RT   identifies a thiolprotease in tomato.";
RL   Plant Physiol. 87:431-436(1988).
CC   -!- INDUCTION: In response to low temperature.
CC   -!- SIMILARITY: Belongs to the peptidase C1 family. {ECO:0000255|PROSITE-
CC       ProRule:PRU10088, ECO:0000255|PROSITE-ProRule:PRU10089}.
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DR   EMBL; M21444; AAA66308.1; -; mRNA.
DR   PIR; JA0159; JA0159.
DR   AlphaFoldDB; P20721; -.
DR   SMR; P20721; -.
DR   STRING; 4081.Solyc12g088670.1.1; -.
DR   MEROPS; C01.029; -.
DR   PaxDb; P20721; -.
DR   PRIDE; P20721; -.
DR   eggNOG; KOG1543; Eukaryota.
DR   eggNOG; KOG4296; Eukaryota.
DR   Proteomes; UP000004994; Unplaced.
DR   ExpressionAtlas; P20721; baseline and differential.
DR   GO; GO:0005615; C:extracellular space; IBA:GO_Central.
DR   GO; GO:0005764; C:lysosome; IBA:GO_Central.
DR   GO; GO:0004197; F:cysteine-type endopeptidase activity; IBA:GO_Central.
DR   GO; GO:0051603; P:proteolysis involved in protein catabolic process; IBA:GO_Central.
DR   CDD; cd02248; Peptidase_C1A; 1.
DR   Gene3D; 2.10.25.160; -; 1.
DR   InterPro; IPR000118; Granulin.
DR   InterPro; IPR037277; Granulin_sf.
DR   InterPro; IPR038765; Papain-like_cys_pep_sf.
DR   InterPro; IPR000169; Pept_cys_AS.
DR   InterPro; IPR025660; Pept_his_AS.
DR   InterPro; IPR000668; Peptidase_C1A_C.
DR   InterPro; IPR039417; Peptidase_C1A_papain-like.
DR   Pfam; PF00396; Granulin; 1.
DR   Pfam; PF00112; Peptidase_C1; 1.
DR   PRINTS; PR00705; PAPAIN.
DR   SMART; SM00277; GRAN; 1.
DR   SMART; SM00645; Pept_C1; 1.
DR   SUPFAM; SSF54001; SSF54001; 1.
DR   PROSITE; PS00139; THIOL_PROTEASE_CYS; 1.
DR   PROSITE; PS00639; THIOL_PROTEASE_HIS; 1.
PE   2: Evidence at transcript level;
KW   Disulfide bond; Glycoprotein; Hydrolase; Protease; Reference proteome;
KW   Stress response; Thiol protease; Zymogen.
FT   PROPEP          <1..17
FT                   /note="Activation peptide"
FT                   /evidence="ECO:0000255"
FT                   /id="PRO_0000026424"
FT   CHAIN           18..237
FT                   /note="Low-temperature-induced cysteine proteinase"
FT                   /id="PRO_0000026425"
FT   PROPEP          238..346
FT                   /note="Removed in mature form"
FT                   /evidence="ECO:0000250"
FT                   /id="PRO_0000046019"
FT   ACT_SITE        42
FT                   /evidence="ECO:0000250"
FT   ACT_SITE        178
FT                   /evidence="ECO:0000250"
FT   ACT_SITE        198
FT                   /evidence="ECO:0000250"
FT   CARBOHYD        215
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   DISULFID        39..81
FT                   /evidence="ECO:0000250"
FT   DISULFID        73..114
FT                   /evidence="ECO:0000250"
FT   DISULFID        172..223
FT                   /evidence="ECO:0000250"
FT   DISULFID        256..268
FT                   /evidence="ECO:0000250"
FT   DISULFID        262..283
FT                   /evidence="ECO:0000250"
FT   NON_TER         1
SQ   SEQUENCE   346 AA;  37429 MW;  D42AC73944010928 CRC64;
     KLSKNKSDRY LPKVGDSLPE SIDWREKGVL VGVKDQGSCG SCWAFSAVAA MESINAIVTG
     NLISLSEQEL VDCDRSYNEG CDGGLMDYAF EFVIKNGGID TEEDYPYKER NGVCDQYRKN
     AKVVKIDSYE DVPVNNEKAL QKAVAHQPVS IALEAGGRDF QHYKSGIFTG KCGTAVDHGV
     VIAGYGTENG MDYWIVRNSW GANCRENGYL RVQRNVSSSS GLCGLAIEPS YPVKTGPNPP
     KPAPSPPSPV KPPTECDEYS QCAVGTTCCC ILQFRRSCFS WGCCPLEGAT CCEDHYSCCP
     HDYPICNVRQ GTCSMSKGNP LGVKAMKRIL AQPIGAFGNG GKKSSS