ID   CYST_CHLAT              Reviewed;         286 AA.
AC   A2CI71;
DT   10-JUL-2007, integrated into UniProtKB/Swiss-Prot.
DT   20-FEB-2007, sequence version 1.
DT   25-MAY-2022, entry version 48.
DE   RecName: Full=Probable sulfate transport system permease protein cysT;
GN   Name=cysT;
OS   Chlorokybus atmophyticus (Soil alga).
OG   Plastid; Chloroplast.
OC   Eukaryota; Viridiplantae; Streptophyta; Chlorokybophyceae; Chlorokybales;
OC   Chlorokybaceae; Chlorokybus.
OX   NCBI_TaxID=3144;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=SAG 48.80;
RX   PubMed=17222354; DOI=10.1186/1741-7007-5-2;
RA   Lemieux C., Otis C., Turmel M.;
RT   "A clade uniting the green algae Mesostigma viride and Chlorokybus
RT   atmophyticus represents the deepest branch of the Streptophyta in
RT   chloroplast genome-based phylogenies.";
RL   BMC Biol. 5:2-2(2007).
CC   -!- FUNCTION: Part of the ABC transporter complex cysAWTP (TC 3.A.1.6.1)
CC       involved in sulfate/thiosulfate import. Probably responsible for the
CC       translocation of the substrate across the membrane (By similarity).
CC       {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Plastid, chloroplast membrane {ECO:0000305};
CC       Multi-pass membrane protein {ECO:0000305}.
CC   -!- SIMILARITY: Belongs to the binding-protein-dependent transport system
CC       permease family. CysTW subfamily. {ECO:0000305}.
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DR   EMBL; DQ422812; ABM87953.1; -; Genomic_DNA.
DR   RefSeq; YP_001019170.1; NC_008822.1.
DR   AlphaFoldDB; A2CI71; -.
DR   SMR; A2CI71; -.
DR   GeneID; 4783323; -.
DR   GO; GO:0031969; C:chloroplast membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005886; C:plasma membrane; IEA:InterPro.
DR   GO; GO:0015419; F:ABC-type sulfate transporter activity; IEA:InterPro.
DR   CDD; cd06261; TM_PBP2; 1.
DR   Gene3D; 1.10.3720.10; -; 1.
DR   InterPro; IPR011865; CysT_permease.
DR   InterPro; IPR000515; MetI-like.
DR   InterPro; IPR035906; MetI-like_sf.
DR   InterPro; IPR005667; Sulph_transpt2.
DR   PANTHER; PTHR30406; PTHR30406; 1.
DR   PANTHER; PTHR30406:SF8; PTHR30406:SF8; 1.
DR   Pfam; PF00528; BPD_transp_1; 1.
DR   SUPFAM; SSF161098; SSF161098; 1.
DR   TIGRFAMs; TIGR00969; 3a0106s02; 1.
DR   TIGRFAMs; TIGR02139; permease_CysT; 1.
DR   PROSITE; PS50928; ABC_TM1; 1.
PE   3: Inferred from homology;
KW   Chloroplast; Membrane; Plastid; Sulfate transport; Transmembrane;
KW   Transmembrane helix; Transport.
FT   CHAIN           1..286
FT                   /note="Probable sulfate transport system permease protein
FT                   cysT"
FT                   /id="PRO_0000293975"
FT   TRANSMEM        27..47
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00441"
FT   TRANSMEM        77..97
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00441"
FT   TRANSMEM        106..126
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00441"
FT   TRANSMEM        147..167
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00441"
FT   TRANSMEM        196..216
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00441"
FT   TRANSMEM        225..245
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00441"
FT   TRANSMEM        257..277
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00441"
FT   DOMAIN          71..272
FT                   /note="ABC transmembrane type-1"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00441"
SQ   SEQUENCE   286 AA;  31812 MW;  B4F8A71B5B87976F CRC64;
     MSTNEMNQKK RLNRSGSLSS HLTRSWPWQL TLSYLFFMLI LPVIALLSRA SDELFKDFWQ
     IAAEPVAIST YVVTLMTALF ATLINGFFGV IIAWVLVRYN FPGKRIIDAA IDLPFALPTS
     VAGLTLATVY SDQGWIGHLF ESIGIKVAFT RVGVAVAMIF VSFPFVVRTL QPVLVEIDQE
     LEEAAWSLGA STWRTFWRVI FPPLTPAIVT GVALAFSRAI GEYGSVVIVA SNIPFKDLTA
     PVLIFQRLEQ YDYTGATIIG TVILSISLFL LFGINFIQSL NQLYVK