CYST_CHLVU
ID CYST_CHLVU Reviewed; 266 AA.
AC P56343;
DT 15-JUL-1998, integrated into UniProtKB/Swiss-Prot.
DT 15-JUL-1998, sequence version 1.
DT 25-MAY-2022, entry version 75.
DE RecName: Full=Probable sulfate transport system permease protein cysT;
GN Name=cysT;
OS Chlorella vulgaris (Green alga).
OG Plastid; Chloroplast.
OC Eukaryota; Viridiplantae; Chlorophyta; core chlorophytes; Trebouxiophyceae;
OC Chlorellales; Chlorellaceae; Chlorella clade; Chlorella.
OX NCBI_TaxID=3077;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=IAM C-27 / Tamiya;
RX PubMed=9159184; DOI=10.1073/pnas.94.11.5967;
RA Wakasugi T., Nagai T., Kapoor M., Sugita M., Ito M., Ito S., Tsudzuki J.,
RA Nakashima K., Tsudzuki T., Suzuki Y., Hamada A., Ohta T., Inamura A.,
RA Yoshinaga K., Sugiura M.;
RT "Complete nucleotide sequence of the chloroplast genome from the green alga
RT Chlorella vulgaris: the existence of genes possibly involved in chloroplast
RT division.";
RL Proc. Natl. Acad. Sci. U.S.A. 94:5967-5972(1997).
CC -!- FUNCTION: Part of the ABC transporter complex cysAWTP (TC 3.A.1.6.1)
CC involved in sulfate/thiosulfate import. Probably responsible for the
CC translocation of the substrate across the membrane (By similarity).
CC {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Plastid, chloroplast membrane {ECO:0000305};
CC Multi-pass membrane protein {ECO:0000305}.
CC -!- SIMILARITY: Belongs to the binding-protein-dependent transport system
CC permease family. CysTW subfamily. {ECO:0000305}.
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DR EMBL; AB001684; BAA57966.1; -; Genomic_DNA.
DR PIR; T07318; T07318.
DR RefSeq; NP_045890.1; NC_001865.1.
DR AlphaFoldDB; P56343; -.
DR SMR; P56343; -.
DR GeneID; 809121; -.
DR GO; GO:0031969; C:chloroplast membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005886; C:plasma membrane; IEA:InterPro.
DR GO; GO:0015419; F:ABC-type sulfate transporter activity; IEA:InterPro.
DR CDD; cd06261; TM_PBP2; 1.
DR Gene3D; 1.10.3720.10; -; 1.
DR InterPro; IPR011865; CysT_permease.
DR InterPro; IPR000515; MetI-like.
DR InterPro; IPR035906; MetI-like_sf.
DR InterPro; IPR005667; Sulph_transpt2.
DR PANTHER; PTHR30406; PTHR30406; 1.
DR PANTHER; PTHR30406:SF8; PTHR30406:SF8; 1.
DR Pfam; PF00528; BPD_transp_1; 1.
DR SUPFAM; SSF161098; SSF161098; 1.
DR TIGRFAMs; TIGR00969; 3a0106s02; 1.
DR TIGRFAMs; TIGR02139; permease_CysT; 1.
DR PROSITE; PS50928; ABC_TM1; 1.
PE 3: Inferred from homology;
KW Chloroplast; Membrane; Plastid; Sulfate transport; Transmembrane;
KW Transmembrane helix; Transport.
FT CHAIN 1..266
FT /note="Probable sulfate transport system permease protein
FT cysT"
FT /id="PRO_0000059994"
FT TRANSMEM 12..32
FT /note="Helical"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00441"
FT TRANSMEM 59..79
FT /note="Helical"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00441"
FT TRANSMEM 91..111
FT /note="Helical"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00441"
FT TRANSMEM 129..149
FT /note="Helical"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00441"
FT TRANSMEM 181..201
FT /note="Helical"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00441"
FT TRANSMEM 206..226
FT /note="Helical"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00441"
FT TRANSMEM 236..256
FT /note="Helical"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00441"
FT DOMAIN 53..257
FT /note="ABC transmembrane type-1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00441"
SQ SEQUENCE 266 AA; 30116 MW; C6456481B18E1042 CRC64;
MKRYPTFIKN SILLFYFFFL LILPVVVLFL LIFQNNWHEV LRKATDPIAV SAYLLTVQMA
FYAALVNSIF GFIITWVLTR YQFWGREFLD AAVDLPFALP TSVAGLTLAT VYGDQGWIGS
LFNLFGFQIV FTKIGVLLAM IFVSFPFVIR TLQPVLQEME KSLEEAAWSL GASSWETFRK
VILPTLWPAL FTGFTLSFSR ALGEFGSIVM ISSNLPFKDL VASVLIYQSL EQYDYLGASV
IGAVVLLIAL FTLLLINAFQ IMKFRV
