CYST_ECOLI
ID CYST_ECOLI Reviewed; 277 AA.
AC P16701;
DT 01-AUG-1990, integrated into UniProtKB/Swiss-Prot.
DT 01-AUG-1990, sequence version 1.
DT 03-AUG-2022, entry version 167.
DE RecName: Full=Sulfate transport system permease protein CysT;
GN Name=cysU; Synonyms=cysT; OrderedLocusNames=b2424, JW2417;
OS Escherichia coli (strain K12).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC Enterobacteriaceae; Escherichia.
OX NCBI_TaxID=83333;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=K12;
RX PubMed=2188958; DOI=10.1128/jb.172.6.3351-3357.1990;
RA Sirko A., Hryniewicz M.M., Hulanicka D.M., Boeck A.;
RT "Sulfate and thiosulfate transport in Escherichia coli K-12: nucleotide
RT sequence and expression of the cysTWAM gene cluster.";
RL J. Bacteriol. 172:3351-3357(1990).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX PubMed=9205837; DOI=10.1093/dnares/4.2.91;
RA Yamamoto Y., Aiba H., Baba T., Hayashi K., Inada T., Isono K., Itoh T.,
RA Kimura S., Kitagawa M., Makino K., Miki T., Mitsuhashi N., Mizobuchi K.,
RA Mori H., Nakade S., Nakamura Y., Nashimoto H., Oshima T., Oyama S.,
RA Saito N., Sampei G., Satoh Y., Sivasundaram S., Tagami H., Takahashi H.,
RA Takeda J., Takemoto K., Uehara K., Wada C., Yamagata S., Horiuchi T.;
RT "Construction of a contiguous 874-kb sequence of the Escherichia coli-K12
RT genome corresponding to 50.0-68.8 min on the linkage map and analysis of
RT its sequence features.";
RL DNA Res. 4:91-113(1997).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / MG1655 / ATCC 47076;
RX PubMed=9278503; DOI=10.1126/science.277.5331.1453;
RA Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V.,
RA Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F.,
RA Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B.,
RA Shao Y.;
RT "The complete genome sequence of Escherichia coli K-12.";
RL Science 277:1453-1462(1997).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX PubMed=16738553; DOI=10.1038/msb4100049;
RA Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S.,
RA Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.;
RT "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655
RT and W3110.";
RL Mol. Syst. Biol. 2:E1-E5(2006).
RN [5]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-133.
RX PubMed=2188959; DOI=10.1128/jb.172.6.3358-3366.1990;
RA Hryniewicz M.M., Sirko A., Palucha A., Boeck A., Hulanicka D.M.;
RT "Sulfate and thiosulfate transport in Escherichia coli K-12: identification
RT of a gene encoding a novel protein involved in thiosulfate binding.";
RL J. Bacteriol. 172:3358-3366(1990).
RN [6]
RP SUBCELLULAR LOCATION.
RC STRAIN=K12 / MG1655 / ATCC 47076;
RX PubMed=15919996; DOI=10.1126/science.1109730;
RA Daley D.O., Rapp M., Granseth E., Melen K., Drew D., von Heijne G.;
RT "Global topology analysis of the Escherichia coli inner membrane
RT proteome.";
RL Science 308:1321-1323(2005).
CC -!- FUNCTION: Part of the ABC transporter complex CysAWTP (TC 3.A.1.6.1)
CC involved in sulfate/thiosulfate import. Probably responsible for the
CC translocation of the substrate across the membrane.
CC -!- SUBUNIT: The complex is composed of two ATP-binding proteins (CysA),
CC two transmembrane proteins (CysT and CysW) and a solute-binding protein
CC (CysP). {ECO:0000305}.
CC -!- SUBCELLULAR LOCATION: Cell inner membrane
CC {ECO:0000269|PubMed:15919996}; Multi-pass membrane protein
CC {ECO:0000255|PROSITE-ProRule:PRU00441, ECO:0000269|PubMed:15919996}.
CC -!- SIMILARITY: Belongs to the binding-protein-dependent transport system
CC permease family. CysTW subfamily. {ECO:0000305}.
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DR EMBL; M32101; AAA23637.1; -; Genomic_DNA.
DR EMBL; U00096; AAC75477.1; -; Genomic_DNA.
DR EMBL; AP009048; BAA16298.1; -; Genomic_DNA.
DR PIR; A35402; QRECST.
DR RefSeq; NP_416919.1; NC_000913.3.
DR RefSeq; WP_000458406.1; NZ_SSUR01000044.1.
DR AlphaFoldDB; P16701; -.
DR SMR; P16701; -.
DR BioGRID; 4263208; 7.
DR ComplexPortal; CPX-4385; Sulfate/thiosulfate ABC transporter complex, cypP variant.
DR ComplexPortal; CPX-4386; Sulfate/thiosulfate ABC transporter complex, sbp variant.
DR STRING; 511145.b2424; -.
DR TCDB; 3.A.1.6.1; the atp-binding cassette (abc) superfamily.
DR PaxDb; P16701; -.
DR PRIDE; P16701; -.
DR EnsemblBacteria; AAC75477; AAC75477; b2424.
DR EnsemblBacteria; BAA16298; BAA16298; BAA16298.
DR GeneID; 58463123; -.
DR GeneID; 946882; -.
DR KEGG; ecj:JW2417; -.
DR KEGG; eco:b2424; -.
DR PATRIC; fig|1411691.4.peg.4307; -.
DR EchoBASE; EB0194; -.
DR eggNOG; COG0555; Bacteria.
DR HOGENOM; CLU_016047_14_0_6; -.
DR InParanoid; P16701; -.
DR OMA; VFISGNM; -.
DR PhylomeDB; P16701; -.
DR BioCyc; EcoCyc:CYST-MON; -.
DR BioCyc; MetaCyc:CYST-MON; -.
DR PRO; PR:P16701; -.
DR Proteomes; UP000000318; Chromosome.
DR Proteomes; UP000000625; Chromosome.
DR GO; GO:0035796; C:ATP-binding cassette (ABC) transporter complex, transmembrane substrate-binding subunit-containing; IC:ComplexPortal.
DR GO; GO:0016020; C:membrane; IC:ComplexPortal.
DR GO; GO:0005886; C:plasma membrane; IDA:EcoCyc.
DR GO; GO:0015419; F:ABC-type sulfate transporter activity; IEA:InterPro.
DR GO; GO:1902358; P:sulfate transmembrane transport; IC:ComplexPortal.
DR GO; GO:0008272; P:sulfate transport; IMP:EcoCyc.
DR GO; GO:0015709; P:thiosulfate transport; IC:ComplexPortal.
DR CDD; cd06261; TM_PBP2; 1.
DR Gene3D; 1.10.3720.10; -; 1.
DR InterPro; IPR011865; CysT_permease.
DR InterPro; IPR000515; MetI-like.
DR InterPro; IPR035906; MetI-like_sf.
DR InterPro; IPR005667; Sulph_transpt2.
DR PANTHER; PTHR30406; PTHR30406; 1.
DR PANTHER; PTHR30406:SF10; PTHR30406:SF10; 1.
DR Pfam; PF00528; BPD_transp_1; 1.
DR SUPFAM; SSF161098; SSF161098; 1.
DR TIGRFAMs; TIGR00969; 3a0106s02; 1.
DR TIGRFAMs; TIGR02139; permease_CysT; 1.
DR PROSITE; PS50928; ABC_TM1; 1.
PE 3: Inferred from homology;
KW Cell inner membrane; Cell membrane; Membrane; Reference proteome;
KW Sulfate transport; Transmembrane; Transmembrane helix; Transport.
FT CHAIN 1..277
FT /note="Sulfate transport system permease protein CysT"
FT /id="PRO_0000059989"
FT TRANSMEM 17..37
FT /note="Helical"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00441"
FT TRANSMEM 64..84
FT /note="Helical"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00441"
FT TRANSMEM 99..119
FT /note="Helical"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00441"
FT TRANSMEM 136..156
FT /note="Helical"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00441"
FT TRANSMEM 185..205
FT /note="Helical"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00441"
FT TRANSMEM 215..235
FT /note="Helical"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00441"
FT TRANSMEM 243..263
FT /note="Helical"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00441"
FT DOMAIN 60..263
FT /note="ABC transmembrane type-1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00441"
FT CONFLICT 127
FT /note="E -> F (in Ref. 5)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 277 AA; 30292 MW; 1392821B0DE24459 CRC64;
MFAVSSRRVL PGFTLSLGTS LLFVCLILLL PLSALVMQLA QMSWAQYWEV ITNPQVVAAY
KVTLLSAFVA SIFNGVFGLL MAWILTRYRF PGRTLLDALM DLPFALPTAV AGLTLASLFS
VNGFYGEWLA KFDIKVTYTW LGIAVAMAFT SIPFVVRTVQ PVLEELGPEY EEAAETLGAT
RWQSFCKVVL PELSPALVAG VALSFTRSLG EFGAVIFIAG NIAWKTEVTS LMIFVRLQEF
DYPAASAIAS VILAASLLLL FSINTLQSRF GRRVVGH
