ID   CYST_NEPOL              Reviewed;         284 AA.
AC   Q9TKU8;
DT   06-FEB-2007, integrated into UniProtKB/Swiss-Prot.
DT   01-MAY-2000, sequence version 1.
DT   03-AUG-2022, entry version 76.
DE   RecName: Full=Probable sulfate transport system permease protein cysT;
GN   Name=cysT;
OS   Nephroselmis olivacea (Green alga).
OG   Plastid; Chloroplast.
OC   Eukaryota; Viridiplantae; Chlorophyta; Nephroselmidophyceae;
OC   Nephroselmidales; Nephroselmidaceae; Nephroselmis.
OX   NCBI_TaxID=31312;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=NIES-484 / S-N-5-8;
RX   PubMed=10468594; DOI=10.1073/pnas.96.18.10248;
RA   Turmel M., Otis C., Lemieux C.;
RT   "The complete chloroplast DNA sequence of the green alga Nephroselmis
RT   olivacea: insights into the architecture of ancestral chloroplast
RT   genomes.";
RL   Proc. Natl. Acad. Sci. U.S.A. 96:10248-10253(1999).
CC   -!- FUNCTION: Part of the ABC transporter complex cysAWTP (TC 3.A.1.6.1)
CC       involved in sulfate/thiosulfate import. Probably responsible for the
CC       translocation of the substrate across the membrane (By similarity).
CC       {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Plastid, chloroplast membrane {ECO:0000305};
CC       Multi-pass membrane protein {ECO:0000305}.
CC   -!- SIMILARITY: Belongs to the binding-protein-dependent transport system
CC       permease family. CysTW subfamily. {ECO:0000305}.
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DR   EMBL; AF137379; AAD54899.1; -; Genomic_DNA.
DR   RefSeq; NP_050928.1; NC_000927.1.
DR   AlphaFoldDB; Q9TKU8; -.
DR   SMR; Q9TKU8; -.
DR   GeneID; 801934; -.
DR   GO; GO:0031969; C:chloroplast membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005886; C:plasma membrane; IEA:InterPro.
DR   GO; GO:0015419; F:ABC-type sulfate transporter activity; IEA:InterPro.
DR   CDD; cd06261; TM_PBP2; 1.
DR   Gene3D; 1.10.3720.10; -; 1.
DR   InterPro; IPR011865; CysT_permease.
DR   InterPro; IPR000515; MetI-like.
DR   InterPro; IPR035906; MetI-like_sf.
DR   InterPro; IPR005667; Sulph_transpt2.
DR   PANTHER; PTHR30406; PTHR30406; 1.
DR   PANTHER; PTHR30406:SF8; PTHR30406:SF8; 1.
DR   Pfam; PF00528; BPD_transp_1; 1.
DR   SUPFAM; SSF161098; SSF161098; 1.
DR   TIGRFAMs; TIGR00969; 3a0106s02; 1.
DR   TIGRFAMs; TIGR02139; permease_CysT; 1.
DR   PROSITE; PS50928; ABC_TM1; 1.
PE   3: Inferred from homology;
KW   Chloroplast; Membrane; Plastid; Sulfate transport; Transmembrane;
KW   Transmembrane helix; Transport.
FT   CHAIN           1..284
FT                   /note="Probable sulfate transport system permease protein
FT                   cysT"
FT                   /id="PRO_0000277441"
FT   TRANSMEM        25..45
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00441"
FT   TRANSMEM        75..95
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00441"
FT   TRANSMEM        107..127
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00441"
FT   TRANSMEM        145..165
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00441"
FT   TRANSMEM        194..214
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00441"
FT   TRANSMEM        223..243
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00441"
FT   TRANSMEM        255..275
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00441"
FT   DOMAIN          69..272
FT                   /note="ABC transmembrane type-1"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00441"
SQ   SEQUENCE   284 AA;  30903 MW;  8AF2B72181D07F48 CRC64;
     MFDPKSLDSG SRSILTMKNR LVSWAWALTL MYMLVSLILP IGALLQKSSQ ESVSEFVSIA
     TAPVAMSAYA VTLSSALIAA LLNGVFGLLI AWVLVRYEFP GRRLLDAAVD LPFALPTSVA
     GLTLATVYSD QGWIGTWLSS LNIQVAFTRL GVMLAMLFVS FPFVVRTLQP VLQDMERELE
     EAAWSLGASP FNTFLRVLCP PLMPAMMTGI ALAFSRAVGE YGSVVIVSGN IPFQDLIAPV
     LIFQRLEQYD YSGATVIGTV VLLISLTLLL AINWIQASNR KFLG